Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

  • NAAS Rating 5.60

  • SJR 0.293

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Agricultural Research, volume 51 issue 4 (august 2017) : 365-369

Leaf Area Index, Chlorophyll, Photosynthesis rate of Lettuce (Lactuca sativa L) under N-organic Fertilizer

Widyati Slamet, Endang Dwi Purbajanti, Adriani Darmawati, Eny Fuskhah
1<p>Faculty of Animal and Agricultural Sciences, Diponegoro University,&nbsp;Jalan Prof Sudharto SH, Tembalang, Semarang, Indonesia 50275.&nbsp;</p>
Cite article:- Slamet Widyati, Purbajanti Dwi Endang, Darmawati Adriani, Fuskhah Eny (2017). Leaf Area Index, Chlorophyll, Photosynthesis rate of Lettuce(Lactuca sativa L) under N-organic Fertilizer . Indian Journal of Agricultural Research. 51(4): 365-369. doi: 10.18805/ijare.v51i04.8424.

The objective of this research is to assess the growth and physiological characteristics of lettuce in the delivery of different N-organics. Research was conducted at Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia. The materials used were seeds of lettuce (Lettuce sativa L) on Oxisol soil types, with the  total N content of 0.18 %, P (18 mg. g-1), K (23 mg. g-1) and C (1.52 %) were utilized. The organic fertilizers applied were cow-, sheep-, and guano manures, and compost and green manure (Leucaena leucocephala). The experimental design was a  randomized block design with four replications and five treatments. The treatment dose of fertilizer applied is equivalent to 100 kg N / ha, namely: cow-manure ; sheep-manure; guano manure ; compost and green manure. Green manure, set into the ground is Leucaena leucocephala. The variables measured were the number of leaves, leaf area, leaf area index(LAI), the rate of photosynthesis,  Chlorophyll  total, anti-oxidant activity,  fresh weight of lettuce per plot. The data obtained were analyzed  using an ANOVA followed by the Duncan test. Guano fertilizer provided the most excellent effect on leaves, LAI, the  rate of photosynthesis and biomass lettuce. Fertilizers derived from animal manure (guano, cattle, goats) gave a more excellent effect on the rate of photosynthesis of plants. Compost origin gave the lowest effect on leaf area, LAI,  and the rate of photosynthesis. The number of leaves, total chlorophyll, and antioxidant content were not influenced by the different organic N fertilizers.


  1. Al Mohammedi, A.N., Almehemdi, A.F., AL Ajeelee, R. K. (2014). Impact of Bat Guano Otonycteris hemprichii Camd and Seaweed Extract on Some Growth and Yield Traits of Barakaseed Nigella Sativa L. Journal of Biology, Agriculture and Healthcare. 4: 57-65.

  2. Ali, W., Hamiduddin, Ahmad. A., Aslam, M., Nasir, A.(2016) Tukh-E-Kahu (Lactuca sativa LINN): Pharmacological and Phytochemical Profile and Uses is Unani Medicine. J.Pharm.Sci.Innov. 5(1):1-4.

  3. Allott, A. (2001). Biology for the I B Diploma, standard and Higher Level.Oxford Univ Press. P141.

  4. Anbukkarasi, V. and Sadasakthi, A. (2017). Effect of leguminous green leaf manures and leaf extract on growth, yield, quality and economics of bhendi [Abelmoschus esculentus (L.) Moench]cv. Arka Anamika. Indian J. Agric. Res.,51 (1): 9-16.

  5. Bernier, J.N., Undi, M., Ominski, K.H., Donohoe, G., Tenuta, M., Flaten, D., Plaizier, J.C., Wittenberg, K.M. (2014). Nitrogen and phosphorus utilization and excretion by beef cows fed a low quality forage diet supplemented with dried distillers grains with solubles under thermal neutral and prolonged cold conditions. Animal Feed with solubles Science and Technology 193: 9–20.

  6. Bharambe C. M. (2015). Comparative Assesment of Bat Guano as an Excelent Bio Compost in Reference to NPK. 2(2) : 20-21.

  7. Chen,H., Chen, J., Yang, H., Chen, W., Gao, H., Lu, W. (2016). Variation in total anthocyanin, phenolic contents, antioxidant enzyme and antioxidant capacity among different mulberry (Morus sp.) cultivars in China Scientia Horticulturae 213: 186–192.

  8. Dadiga,A., Kadwey, S. and Prajapati, S. (2015). Influences of organic and inorganic sources of nutrients on growth, yield attributed traits and yield economic of coriander(Coriandrum sativum L.)cv JD-1. Indian J. Agric. Res.,49(6):577-580.

  9. Gan, Y. Z. and Azrina, A. (2016) Antioxidant properties of selected varieties of lettuce(Lactuca sativa L.) commercially available in Malaysia International Food Research Journal 23(6): 2357-2362 .

  10. Hariadi, Y.C., Nurhayati, A. Y., Hariyani, P. (2015). Biophysical Monitoring on the Effect on Different Composition of Goat and Cow Manure on the Growth Response of Maize to Support Sustainability. Agriculture and Agricultural Science Procedia 9: 118 – 127.

  11. Hasan, A.E., Bhiah, K.M. and Al-Zurfy, M. T. H. (2014). The impact of peat moss and sheep manure compost extracts on marigold (Calendula officinalis L.) growth and flowering. Journal of Organic Systems, 9(2), 2014. 56-62.

  12. Kim, M., Moon, Y., Tou, J.C., Mou, B., Waterland, N. (2016). Nutritional value, bioactive Compounds and health benefits of lettuce (Lactuca sativa L.). Journal of Food Composition and Analysis. 49:19-34. doi: 10.1016/j.jfca.2016.03.004.

  13. Koleva II, Van Beek T.A., Linssen J.P.H., de Groot A, Evstatieva L.N. (2002). Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis 13: 8-17. 

  14. Limantara,L., Dettling, M., Indrawati, R., Indriatmoko, T. H., Brotosudarmo, P. (2015). Analysis on the Chlorophyll Content of Commercial Green Leafy. Procedia Chemistry 14: 225-231.

  15. Pedersen,O., Colmer, T. D. and Sand-Jensen, K. (2013). Underwater photosynthesis of submerged plants – recent advances and methods. Frontiers in Plant Science , Plant Physiology, 140: 1-19.

  16. Purbajanti, E.D., Setiadi, A. and Roessali, W. (2016). Variability and nutritive compounds of guava (Psidium guajava L.) Indian J. Agric. Res.,50(3):273-277.

  17. Purbajanti, E.D., Kusmiyati, F., Slamet, W., Adinurani., P.G. (2016). Chlorophyll, crop growth rate and forage yield of brachiaria (Brachiaria brizantha Stapf) as the result of goat manure in various nitrogen dosage. AIP Conf. Proc.1755, 130013 (2016); 10.1063/1.4958557.

  18. Rajalakshmi .K, and Banu. N. (2015). Extraction and Estimation of Chlorophyll from Medicinal Plants. International Journal of Science and Research (IJSR). 4: 209-212.

  19. Rashed,N.M. and R.Kh. Darwesh . 2015. A comparative study on the effect of microclimate on planting date and water requirements under different nitrogen sources on coriander (Coriandrum sativum, L.) Annals of Agricultural Science60(2), 227–243.

  20. Sharma, R.K., Sharma, S.K. and Dangi, N.L. Influence of different organic nutrient sources on productivity and profitability of groundnut (Arachis hypogaea L.) in southern Rajasthan, India. Indian J. Agric. Res., 50 (6) : 623-626.

  21. Steel, R.G.D and Torrie, J.H. (1960). Principle and Procedures of Statistics. Mc. GrawHill Inc. New York. 

  22. Stopes , C., Millington, S., Woodward, L. (1996). Dry matter and nitrogen accumulation by three leguminous green manure species and the yield of a following wheat crop in an organic production system. Agriculture, Ecosystems and Environment 57 : I89- 196.

  23. Subaedah, S., Aladin, A. and Nirwana. (2016). Fertilization of Nitrogen, Phosphor and Application of Green Manure of Crotalaria juncea In Increasing Yield of Maize In Marginal Dry Land. Agriculture and Agricultural Science Procedia 9: 20 – 25.

  24. Tiemens-Hulscher ,M., Lammerts, E. T. van Bueren, Struik, P. C. (2014). Identifying nitrogen-efficient potato cultivars for organic farming. Euphytica 199:137–154. 

Editorial Board

View all (0)