Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

  • NAAS Rating 5.60

  • SJR 0.293

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Agricultural Research, volume 50 issue 1 (february 2016) : 15-21

Screening for active compounds in Rhus coriaria L. crude extract that inhibit the growth of Pseudomonas syringae and Ralstonia solanacearum

Tavga Sulaiman Rashid1, Kamaruzaman Sijam, Jugah Kadir, Halimi Mohd Saud, Hayman Kakakhan Awla, Dzarifah Zulperi, Erneeza Mohd Hata
1<p>Department of Plant Protection, Faculty of Agriculture, University Putra Malaysia,&nbsp;UPM, Serdang, Selangor Darul Ehsan 43400, Malaysia</p>
Cite article:- Rashid1 Sulaiman Tavga, Sijam Kamaruzaman, Kadir Jugah, Saud Mohd Halimi, Awla Kakakhan Hayman, Zulperi Dzarifah, Hata Mohd Erneeza (2016). Screening for active compounds in Rhus coriaria L. crude extract that inhibit the growth of Pseudomonas syringae and Ralstonia solanacearum . Indian Journal of Agricultural Research. 50(1): 15-21. doi: 10.18805/ijare.v50i1.8583.

An experiment was performed to study the antibacterial activity of methanol, acetone, alcohol and aqueous extracts from the fruit of R. coriaria by disk diffusion assay in terms of minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC) and killing-time curve. The detection of the components was also fulfilled using Gas Chromatography–Mass Spectrometry (GC-MS) and also tested for their antibacterial activity. The tested bacteria were Pseudomonas syringae (Accession No. KJ858057), a tomato bacterial speck causal agent, and Ralstonia solanacearum (Accession No. KJ881159) causing tomato bacterial wilt. Furthermore, the inhibition criteria were made by different extracts of the sampled bacteria which were measured and compared with standard antibiotic (chloramphenicol). Aqueous extract displayed better outcomes against P. syringae and R. solanacearum as compared to chloramphenicol. According to the GC-MS test results, the aqueous extract was composed of 39 different phytocompounds, together with eight elements in the high peak region, namely Furfural, 1-Cyclopetene, 2,5 Furandione,  Phloroglucinol, Succinic acid, Malic acid, P-Tolylacetic acid and  Coumalic acid. Among these, it was discovered that 2,5 Furandione was the most important antibacterial element that is present in sumac. The results from the current study indicate that different extracts of R. coriaria contain a variety of antibacterial compounds which can potentially be used to produce an extensive range of herbal mixtures with anti-bacterial properties for controlling diseases in crops belonging to the Solanaceae family. 

  1. Baretta, G.; Rossoni, G.; Santagati, N.A. and Facino, R.M. (2009) Anti-ischemic activity and endothelium dependent vasorelaxant effect of hydrolysable tannins from the leaves of Rhus coriaria (Sumac) in isolated rabbit heart and thoracic aorta. Planta. Med., 75: 1482-1488.

  2. Barsi, D.F.and Fan, S.H. ( 2005) The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Indian J. Pharmacol., 37: 26-29.

  3. Chakraborty, A.; Ferk, F.; Simic, T.; Branter, A.; Dusinska, M.; Kundi, M.C.; Nersesvan N. and Knasmuller, S. (2009) DNA-protective effects of sumach (Rhus coriaria L.), a common spice: Results of human and animal studies. Mut. Res., 661: 10-17.

  4. Dai, J. and Mumper, R.J. (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Mol., 15:7313-7352.

  5. Fazeli, M.R.; Amin, G.;, Attari, M.M.A.;, Ashtiani, H.;, Jamalifar, H. and Samadi, N. (2007) Antimicrobial activities of Iranian sumac and avishan-e shirazi (Zataria multiXora) against some food-borne bacteria. Food. Cont., 

  6. 18: 646-649.

  7. Gao, Z.; Shao, J.; Sun, H.; Zhong, W.; Zhuang, W. and Zhang, Z. (2012) Evaluation of different kinds of organic acids and their antibacterial activity in Japanese Apricot fruits. African Journal of Agricultural Research., 7: 4911-4918.

  8. Hashem, M. and Alamri, S. (2010) Contamination of common spices in Saudi Arabia markets with potential mycotoxin-    producing fungi. Saudi. J. Biol. Sci., 17: 167-175.

  9. Herrmann, K. (1988) On the occurrence of flavonol and flavone glycosides in vegetables. Z. Lebensm. Unters. Forsch., 186:1–5.

  10. Kosar, M.; Bozan, B.; Temelli, F. and Baser, K.H.C. (2007) Antioxidant activity and phenolic composition of sumac (Rhus coriaria L.) extracts. Food Chem., 103:952-959.

  11. Kossah, R.; Nsabimana. C.; Zhang, H. and Chen, W. (2010) Optimization of extraction of polyphenols from Syrian sumac (Rhus coriaria L.) and Chinese sumac (Rhus typhina L.) fruits. Res. J. Phytochem ., 4: 146-153.

  12. Kossah, R.; Nsabimana, C.; Zhao, J.X.; Chen, H.Q. Tian, F.W.; Zhang, H. and Chen, W. (2009) Comparative study on the chemical composition of Syrian sumac (Rhus coriaria L.) and Chinese sumac (Rhus typhina L.) fruits. Pak. J. Nutr., 8: 1570-1574.

  13. Lorian, V. (2005) Antimicrobial susceptibility on solid media. In Antibiotic in Laboratory Medicine. Lippincott William & Wilkins .Fifth edition. USA., pp 8-61.

  14. Marino, M.; Bersani, C. and Comi, M. (1999) Antimicrobial activity of the essential oils of Thymus vulgaris L. measure using a bioimpediometric method. Journal of Food Protection.,62:1017– 1023.

  15. Mokbel, M.S. and Hashinaga, F. (2005) Evaluation of the Antimicrobial Activity of Extract from limitan (Citrus grandis Osbeck) Fruit Peel. Pakistan Journal of Biological Sciences.,8: 1090-1095.

  16. Nasar-Abbas, S.M. and Halkman, A.K. (2004) Antimicrobial effect of water extract of sumac (Rhus coriaria L.) on the growth of some food-borne bacteria including pathogens. Int. J. Food Microbiol., 97: 63-69.

  17. Panico, A.; Cardile, V.; Santagati, N.A. and Messina, R. (2009) Antioxidant and protective effects of sumac leaves on chondrocytes. J. Med. Plants. Res., 3: 855-861.

  18. Pankey, G.A. and Ashcraft, D.S. (2009) In vitro antibacterial activity of tigecycline against resistant Gram-negative bacilli and enterococci by time-kill assay. Diagnosis Microbiology of Infectious Disease., 64: 300-304.

  19. Pourahmad, J.; Eskandari, M.R. Shakibaei, Rand Kamalinejad, M. (2010) A search for hepatoprotective activity of aqueous extract of Rhus coriaria L. against oxidative stress cytotoxicity. Food. Chem. Toxicol., 48: 854-858.

  20. Prabuseenivasan, S.; Jayakumar, M. and Ignacimuthu, S. (2006) In vitro antibacterial activity of some plant essential oils. BMC Complem. Altern. Med., 6: 39.

  21. Raybaudi-Massilia, R.M.; Mosqueda-Melgar, J. and Martín-Belloso, O. (2009) Antimicrobial activity of malic acid against Listeria monocytogenes, Salmonella enteritidis and Escherichia coli O157: H7 in apple, pear and melon juices. Food Control., 20: 105-112.

  22. Singleton, V.L. (1981) Naturally occurring food toxicants: phenolic substancesof plant origin common in foods. Add. Food Res., 27: 149–242

  23. Wangensteen, H.; Samuelsen, A.B. and Malterud, K. E. (2004) Antioxidant activity in extracts from coriander. Food Chemistry., 88: 293 -297.

  24. Winstead, N.N.and Kelman, A. (1952) Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology., 42:628–634.

  25. Zargham, H. and Zargham, R. (2008) Tannin extracted from sumac inhibits vascular smooth muscle cell migration. Mcgill. J. Med., 11: 119-123. 

Editorial Board

View all (0)