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Full Research Article

volume 36 issue 3 (september 2021) : 231-236,   Doi: 10.18805/BKAP348

Evaluation of Genetic Diversity in Ocimum spp. using Agro-morphological Traits and RAPD Marker

S
Sachin Kumar
A
Ajay Kumar
M
Manoj Kumar Sharma
M
Manoj Kumar Sharma
1Department of Bioinformatics, Janta Vedic College, Baraut, Baghpat-250 611, Uttar Pradesh, India.

  • Submitted05-08-2021|

  • Accepted22-09-2021|

  • First Online 28-09-2021|

  • doi 10.18805/BKAP348

Cite article:- Kumar Sachin, Kumar Ajay, Sharma Kumar Manoj, Sharma Kumar Manoj (2021). Evaluation of Genetic Diversity in Ocimum spp. using Agro-morphological Traits and RAPD Marker . Bhartiya Krishi Anusandhan Patrika. 36(3): 231-236. doi: 10.18805/BKAP348.

ABSTRACT

In the present study, morphological and genetic diversity is revealed among the four species of Ocimum. Among the morphological characters plant height, the number of leaves, leaf area, leaf width, leaf length, stem circumference and mitotic index studied and contributed a greater proportion of variations. RAPD markers revealed a high degree of polymorphism (88.89%) among the species of Ocimum. Dendrogram study revealed that the species O. gratissimum and O. basilicum are very closely related whereas O. kilimandscharicum and O. sanctum are distantly related in the phylogenetic history. 

REFERENCES


  1. Atienzar, F., Evenden, A., Jha, A., Savva, D. and Depledge, M. (2000). Optimized RAPD analysis generates high quality genomic DNA profiles at high annealing temperatures. Biotechniques. 28: 52-54.

  2. Balyan, S.S. and Pushpangadan, P. (1988). A study of the taxonomical status and geographic distribution of the genus Ocimum. PAFAI. 10(2): 13-19.

  3. Cipriani, G., Bella, R. and Testolin, R. (1996). Screening RAPD primer for molecular taxonomy and cultivar fingerprinting in genus Actinidia. Euphytica. 90: 169-174.

  4. Mishra, D., Awasthi, A. and Mishra, P. (2014). Phylogenetic evolution studies on different varieties of genus Ocimum with special reference to Rewa district of Madhya Pradesh. Sci. Secure J. Biotechnol. 3: 188-197.

  5. Dice, L.R. (1945). Measure of the amount of ecological association between species. Ecology. 26: 297-302.

  6. Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13-15.

  7. Khosla, M.K., (1995). Study of inter-relationship, phylogeny and evolutionary tendencies in genus Ocimum. Ind. J. Genet. 55: 71-83.

  8. Matasyoh, L.G., Wachira, F.N., Kinyua, M.G., Thairu Muigai, A.W. and Mukiama, T.K. (2008). Leaf storage conditions and genomic DNA isolation efficiency is Ocimum gratissimum L. from Kenya. African Journal of Biotechnology. 7: 557-564.

  9. Padmalatha, K., Prasad, M.N.V. (2006). Optimisation of DNA isolation and PCR protocol for RAPD analysis of selected medicinal and aromatic plants of conservation concern from Peninsular India. Afr. J. Biotechnol. 5(3): 230-234.

  10. Pushpangadan, P., Bradu, B.L., Chadha, K.L., Gupta, R. (Eds.) (1995). Advances in Horticulture, Malhotra Publishing House, New Delhi. 

  11. Porter, W.M. and Smith D.H. (1982). Detection of identification errors in germplasm collections. Crop Science. 22: 701-703.

  12. Rohlf, F.J. (1998). NTSyS-p.c. Numerical Taxonomy and Multivariate Analysis System (Version 2.0). Exeter Software Publishers Ltd., Setauket.

  13. Sambrook, J., Maccallum, P. and Russell, D. (2001). Molecular Cloning: A Lab-oratory Manual, 3rd edition, Cold Spring Harbor Laboratory Press.

  14. Singh, A.P., Dwivedi, S., Bharti, S., Srivastava, A., Singh, V. and Khanuja, S.P.S. (2004). Phylogenetic relationships as in Ocimum revealed by RAPD markers. Euphytica. 136: 11-20.

  15. Sneath, P.H.A. and Sokal, R.R. (1973). Numerical Taxonomy. Freeman Press San Francisco, California, USA.

  16. Sobti, S.N. and Pushpangadan, P. (1977). Studies in Genus Ocimum: Cytogenetics, Breeding and Produciton of New Strains of EconomicI. In: Cultivation and Utilization of Medicinal and Aromatic Plants. [C.K. Atal and B.M. Kapoor (ed)]  (Regional Research Laboratory Jammu) pp. 273-285.

  17. Stebbins, G.L. (Jr) (1957). Genetics, evolution and plant breeding. Proc Symp on Genet and Pl Breed in Southeast Asia, Jan. 1957, New Dehli. Ind. J. Genet. 17: 129-141.

  18. Tchatchouang, S., Beng, V.P. and Kuete, V. (2017). Antiemetic African Medicinal Spices and Vegetables Medicinal Spices and Vegetables from Africa - Therapeutic Potential against Metabolic, Inflammatory, Infectious and Systemic Diseases, Academic Press Pages 299-313. https://doi.org/10.1016/ B978-0-12-809286-6.00011-X.

  19. Vieira, R.F. and Simon, J.E. (2000). Chemical characterization of basil (Ocimum spp.) found in the markets and used in traditional medicine in Brazil. Econ. Bot. 54: 207-216. 

  20. Vieira, R.F., Grayer, R., Paton, A. and Simon, J.E. (2001). Genetic diversity of Ocimum gatissimum L. based on volatile oil constituents, flavonoids and RAPD markers. Biochem. Syst. Ecol. 29(3): 287-304.

  21. Welsh, J. and McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18(24): 7213-7218. doi: 10.1093/nar/18.24.7213.

  22. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA polymorphism’s amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18(22): 6531-6535. doi: 10.1093/nar/18.22.6531.
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    Full Research Article

    volume 36 issue 3 (september 2021) : 231-236,   Doi: 10.18805/BKAP348

    Evaluation of Genetic Diversity in Ocimum spp. using Agro-morphological Traits and RAPD Marker

    S
    Sachin Kumar
    A
    Ajay Kumar
    M
    Manoj Kumar Sharma
    M
    Manoj Kumar Sharma
    1Department of Bioinformatics, Janta Vedic College, Baraut, Baghpat-250 611, Uttar Pradesh, India.

    • Submitted05-08-2021|

    • Accepted22-09-2021|

    • First Online 28-09-2021|

    • doi 10.18805/BKAP348

    Cite article:- Kumar Sachin, Kumar Ajay, Sharma Kumar Manoj, Sharma Kumar Manoj (2021). Evaluation of Genetic Diversity in Ocimum spp. using Agro-morphological Traits and RAPD Marker . Bhartiya Krishi Anusandhan Patrika. 36(3): 231-236. doi: 10.18805/BKAP348.

    ABSTRACT

    In the present study, morphological and genetic diversity is revealed among the four species of Ocimum. Among the morphological characters plant height, the number of leaves, leaf area, leaf width, leaf length, stem circumference and mitotic index studied and contributed a greater proportion of variations. RAPD markers revealed a high degree of polymorphism (88.89%) among the species of Ocimum. Dendrogram study revealed that the species O. gratissimum and O. basilicum are very closely related whereas O. kilimandscharicum and O. sanctum are distantly related in the phylogenetic history. 

    REFERENCES


    1. Atienzar, F., Evenden, A., Jha, A., Savva, D. and Depledge, M. (2000). Optimized RAPD analysis generates high quality genomic DNA profiles at high annealing temperatures. Biotechniques. 28: 52-54.

    2. Balyan, S.S. and Pushpangadan, P. (1988). A study of the taxonomical status and geographic distribution of the genus Ocimum. PAFAI. 10(2): 13-19.

    3. Cipriani, G., Bella, R. and Testolin, R. (1996). Screening RAPD primer for molecular taxonomy and cultivar fingerprinting in genus Actinidia. Euphytica. 90: 169-174.

    4. Mishra, D., Awasthi, A. and Mishra, P. (2014). Phylogenetic evolution studies on different varieties of genus Ocimum with special reference to Rewa district of Madhya Pradesh. Sci. Secure J. Biotechnol. 3: 188-197.

    5. Dice, L.R. (1945). Measure of the amount of ecological association between species. Ecology. 26: 297-302.

    6. Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13-15.

    7. Khosla, M.K., (1995). Study of inter-relationship, phylogeny and evolutionary tendencies in genus Ocimum. Ind. J. Genet. 55: 71-83.

    8. Matasyoh, L.G., Wachira, F.N., Kinyua, M.G., Thairu Muigai, A.W. and Mukiama, T.K. (2008). Leaf storage conditions and genomic DNA isolation efficiency is Ocimum gratissimum L. from Kenya. African Journal of Biotechnology. 7: 557-564.

    9. Padmalatha, K., Prasad, M.N.V. (2006). Optimisation of DNA isolation and PCR protocol for RAPD analysis of selected medicinal and aromatic plants of conservation concern from Peninsular India. Afr. J. Biotechnol. 5(3): 230-234.

    10. Pushpangadan, P., Bradu, B.L., Chadha, K.L., Gupta, R. (Eds.) (1995). Advances in Horticulture, Malhotra Publishing House, New Delhi. 

    11. Porter, W.M. and Smith D.H. (1982). Detection of identification errors in germplasm collections. Crop Science. 22: 701-703.

    12. Rohlf, F.J. (1998). NTSyS-p.c. Numerical Taxonomy and Multivariate Analysis System (Version 2.0). Exeter Software Publishers Ltd., Setauket.

    13. Sambrook, J., Maccallum, P. and Russell, D. (2001). Molecular Cloning: A Lab-oratory Manual, 3rd edition, Cold Spring Harbor Laboratory Press.

    14. Singh, A.P., Dwivedi, S., Bharti, S., Srivastava, A., Singh, V. and Khanuja, S.P.S. (2004). Phylogenetic relationships as in Ocimum revealed by RAPD markers. Euphytica. 136: 11-20.

    15. Sneath, P.H.A. and Sokal, R.R. (1973). Numerical Taxonomy. Freeman Press San Francisco, California, USA.

    16. Sobti, S.N. and Pushpangadan, P. (1977). Studies in Genus Ocimum: Cytogenetics, Breeding and Produciton of New Strains of EconomicI. In: Cultivation and Utilization of Medicinal and Aromatic Plants. [C.K. Atal and B.M. Kapoor (ed)]  (Regional Research Laboratory Jammu) pp. 273-285.

    17. Stebbins, G.L. (Jr) (1957). Genetics, evolution and plant breeding. Proc Symp on Genet and Pl Breed in Southeast Asia, Jan. 1957, New Dehli. Ind. J. Genet. 17: 129-141.

    18. Tchatchouang, S., Beng, V.P. and Kuete, V. (2017). Antiemetic African Medicinal Spices and Vegetables Medicinal Spices and Vegetables from Africa - Therapeutic Potential against Metabolic, Inflammatory, Infectious and Systemic Diseases, Academic Press Pages 299-313. https://doi.org/10.1016/ B978-0-12-809286-6.00011-X.

    19. Vieira, R.F. and Simon, J.E. (2000). Chemical characterization of basil (Ocimum spp.) found in the markets and used in traditional medicine in Brazil. Econ. Bot. 54: 207-216. 

    20. Vieira, R.F., Grayer, R., Paton, A. and Simon, J.E. (2001). Genetic diversity of Ocimum gatissimum L. based on volatile oil constituents, flavonoids and RAPD markers. Biochem. Syst. Ecol. 29(3): 287-304.

    21. Welsh, J. and McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18(24): 7213-7218. doi: 10.1093/nar/18.24.7213.

    22. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA polymorphism’s amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18(22): 6531-6535. doi: 10.1093/nar/18.22.6531.
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