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Asian Journal of Dairy and Food Research

  • Chief EditorHarjinder Singh

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Research Article

Asian Journal of Dairy and Food Research, volume 37 issue 1 (march 2018) : 7-12

Prevalence study of Salmonella spp. in milk and milk products 
 

Pooja Singh, R.V. Singh, Bhavana Gupta, Sabya Sanchi Tripathi, Kewal Singh Tomar, Sachin Jain, Y.P. Sahni
1Department of Veterinary Public Health and Epidemiology, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.
Cite article:- Singh Pooja, Singh R.V., Gupta Bhavana, Tripathi Sanchi Sabya, Tomar Singh Kewal, Jain Sachin, Sahni Y.P. (2018). Prevalence study of Salmonella spp. in milk and milk products. Asian Journal of Dairy and Food Research. 37(1): 7-12. doi: 10.18805/ajdfr.DR-1252.

ABSTRACT

The present study was carried out to observe the presence of Salmonella in milk and milk products. The investigation of 210 samples revealed presence of 7.61% Salmonella spp.  with higher prevalence in raw milk and dahi - 11.9% each , followed by ice-cream -9.52% and khoa - 4.76%. None of the sample of flavored milk showed Salmonella. In molecular study, 5 (31.2%) isolates were found positive for invA gene. The gene was present in raw milk - 18.75% and 6.25% in khoa and ice-cream each. Salmonella isolates were found sensitive to netillin - 100%, ofloxacin - 87.5%, norfloxacin, ciprofloxacin and nalidixic acid – (62.5% each), streptomycin and amikacin – (56.2% each). The isolates were resistant to nitrofurantoin- 81.2%, tetracycline -75.0%, co-trimoxazole - 68.7%, ampicilline - 56.2% and cefotaxime - 50.0%. The observation displayed unhygienic atmosphere prevailing during production of dairy products which lead to contamination of Salmonella and thus may cause public health problem to the consumers. 

REFERENCES

  1. Abeer, A.A., Azza, S.A., Gouda, H.A., Dardir, A.H. and Ibrahim, A.K. (2012). Prevalence of some milk borne bacterial pathogens threatening camel milk consumers in Egypt. Global Veterinarian, 8(1): 76-82.
  2. Agarwal, R.K., Bhilegaonkar, K.N., Singh, D.K., Kumar, A. and Rathore, R.S. (2003). Laboratory Manual for the Isolation and Identification of Foodborne Pathogens 1st Edn., Jai Ambey Publishing Co.,U.P. pp. 30-37.
  3. Almeida, C., Cerqueira, L., Azevedo, N. F., and Vieira, M. J. (2013). Detection of Salmonella enterica serovar enteritidis using real time PCR, immunocapture assay, PNA FISH and standard culture methods in different types of food samples. International Journal of Food Microbiology, 161: 16–22.
  4. Anderson, M., Hinds, P., Hurditt, S., Miller, P., Mcgrowder, D. and Ruby Alexander-Lindo, R. (2011). The microbial content of unexpired pasteurized milk from selected supermarkets in a developing country. Asian Pacific Journal Tropical Biomedicine, 1(3): 205–211.
  5. Bauer, A.W., Kirby, W.M., Sherris, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by standard single disk method. American Journal of Clinical Pathology, 45: 493-496.
  6. Bharathy, S., Swetha, C.S. and Sudhanthirakodi, S. (2015). A prospective study on antibiogram pattern for salmonella isolated from poultry origin and milk samples of local chicken retailers and local vendors in Tirupathi, India, International Journal of Agriculture and Science & Veterinary Medicine, 3: 11-16.
  7. Busani, L., Cigliano, A., Taioli, E., Caligiuri, V., Chiavacci, I., Dibella, C., Battisi, A., Duranti, A., Gianfranceschi, M., Nardella, M.C., Ricci, A., Roleus, S., Tamba, M., Marabelli, R and Caprioli, A. (2004). Prevalence of Salmonella enterica and Listeria monocytogenes contamination in foods of animal origin in Italy. Journal of Food Protection. 68: 1729–1733.
  8. CDC (2017): https://www.cdc.gov/features/rawmilk/index.htmlso
  9. Chatli, A.S., Tangri, R., Chawla, H. and Komal, R. (2014). Microbial quality evaluation of milk products. International Journal of Development Research, 4:623-628.
  10. Chiu, C.H., and Ou, J.T. (1996). Rapid identification of Salmonella serovars in feces by specific detection of virulence gene invA and spvC by an enrichment broth culture-multiplex PCR combination assay. Journal of Clinical Microbiology, 34: 2619-2622.
  11. Chuanchuen, R., Ajariyakhajorn, K., Koowatananukul, C., Wannaprasat, W., Khemtong, S. and Samngamnim, S. (2010). Antimicrobial resistance and virulence genes in Salmonella enterica isolates from dairy cows. Food Borne Pathogens and Diseases, 7(1): 63-69.
  12. Chung, Y.H., Kim, S.Y. and Chang, Y.H. (2003). Prevalence and Antibiotic Susceptibility of Salmonella Isolated from Foods in Korea from 1993 to 2001. Journal of Food Protection, 4: 1154-1157.
  13. Chye, F.K., Abdullah, A and Ayob, M.K. (2004). Bacteriological quality and safety of raw milk in Malaysia. Journal of Food Microbiology, 21: 535-541.
  14. Das, A., Hari, S.S., Shalini, U. and Karthikeyan, M. (2012). Molecular screening of virulence genes from salmonella enterica isolated from commercial food stuffs. Biosciences Biotechnology Research Asia, 9(1):363-369.
  15. FAO (2014). Dairy production and products online http:// www. fao. org/ agriculture/ dairy-gateway/milk-and-milk-products/en/
  16. FSSAI (2015). Microbiological parameter for milk products. old. fssai. gov. in / ... / Draft _Regulation _on_Microbiological_ standards_ milk _and_milk_
  17. Jones, B.D. (2005).Salmonella invasion gene regulation: a story of environmental awareness. Journal of Microbiology, 43:110-7.
  18. Karshima, N.S., Pam, V.A., Bata, S.I., Dung, P.A. and Paman, N.D. (2013). Isolation of Salmonella spp. from milk and locally processed milk products traded for human consumption and associated risk factors in Kanam, Plateau State, Nigeria. Journal of Animal Production Advancement, 3(3): 69-74.
  19. Kaushik, P., Anjay, K.S., Bharti, S.K. and Dayal, S. (2014). Isolation and prevalence of Salmonella from chicken meat and cattle milk collected from local markets of Patna, India. Veterinary World, 7(2): 62-65.
  20. Khan, J., Musaddiq, M. and Budhlani, G.N. (2014). Microbial quality of milk and milk products collected from Akola City of Maharashtra. Indian Journal of Applied Research, 4: 59-62.
  21. Lubote, R., Shahada, F. and Matemu., A. (2014). Prevalence of Salmonella spp. and E. coli in raw milk value chain in Arusha,Tanzania. American Journal of Research Communication, 2(9): 1-13. 
  22. Munsi, M.N., Sarker, R.N., Khatun, R. and Alam, M.K. (2015). Identification and antibiogram study of bacterial species isolated from milk samples of different locations in Bangladesh. Asian Journal of Medical Biological Research, 1(3): 457- 462. 
  23. NIIR Project Consultancy Services (An ISO 9001:2008 Company). www.niir.org/.../milk...dairy-products...milk...milk...milk-milk... milk...dairy...butterm... / (4th June 2015).
  24. Pant, R., Nirwal, S. and Rai, N. (2013). Prevalence of antibiotic resistant bacteria and analysis of microbial quality of raw milk samples collected from different regions of Dehradun. International Journal of Pharmacology Technical Research, 5(2): 368-400.
  25. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, Jones JL, Griffin P.M. (2011) Foodborne illness acquired in the United States- -major pathogens. Emerging Infectious Diseases, 17(1):7-15.
  26. Shanmugasamy, M., Velayutham, T. and Rajeswar, J. (2011). InvA gene specific PCR for detection of salmonella from broilers. Veterinary World, 4(12): 562-564.
  27. Singh, V., Kaushal, S., Tyagi, A. and Sharma, P. (2011). Screening of bacteria responsible for the spoilage of milk. Journal of Chemical and Pharmaceutical Research, 3(4):348-350.
  28. Smith, S.I, Fowora, M.A., Atiba, A., Okopi, J.A., Fingesi, T., Adamu, M.E., Omonigbehin, E.A., Ugo-Ijeh, M.I., Bamidele, M., Odeigah, P. (2015). Molecular detection of some virulence genes in Salmonella spp isolated from food samples in Lagos, Nigeria. Animal and Veterinary Sciences, 3(1): 22-27.
  29. Tajbakhsh, F., Tajbakhsh, E., Momeni, M., Rahimi, E. and Sohrabi, R. (2012). Occurrence and antibiotic resistance of Salmonella spp Isolated from raw cow’s milk from Shahahrekord, Iran. International Journal of Microbiological Research, 3(3): 242-245.
  30. Tangri, R. and Chatli, A.S. (2014). Microbial quality and chemical adulterants evaluation in the raw and pasteurized milk. Asian Journal of Science and Technology, 5: 716-721.
  31. Tesfaw, L., Taye, B., Alemu, S., Alemayehu, H., Sisay, Z. and Negussie, H. (2013). Prevalence and antimicrobial resistance profile of Salmonella isolates from dairy products in Addis Ababa, Ethiopia. International Journal of Genetics, 7(43): 5046-5050.
  32. WHO (2015). http://www.who.int/mediacentre/news/releases/2015/foodborne-disease-estimates/en/ 
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