Asian Journal of Dairy and Food Research, volume 36 issue 2 (june 2017) : 166-169

Studies on absorption of nutrients using intestinal sacs of rats fed on different diets

D. R. Sood*, Shahina Kalim, Rajat Sood
1<p>Department of Biochemistry, CCS Haryana Agricultural University, Hisar-125 004, Haryana, India.</p>
Cite article:- Sood* R. D., Kalim Shahina, Sood Rajat (2017). Studies on absorption of nutrients using intestinal sacs of rats fed on different diets . Asian Journal of Dairy and Food Research. 36(2): 166-169. doi: 10.18805/ajdfr.v36i02.7964.

Absorption of glucose, sucrose, glutamic acid and methionine was studied using the intestinal everted sac technique. Rats were fed with potato tubers of Kufri Ashoka, Kufri Chipsona-1 and Kufri Chipsona-2 in combination with casein in 1:1 ratio and casein as a control at eight per cent, protein level. Absorption of sucrose, glucose, glutamic acid and methionine was monitored spectrophotometrically and of glucose, sucrose and glutamic acid by 14C radio assay using scintillation counting. In general, glutamic acid showed higher absorption values than glucose, sucrose and methionine by both the techniques. Rats fed on casein diet exhibited maximum absorption of glutamic acid and methionine. Incorporation of 10 ?mole of lithium decreased considerably the absorption of glucose, sucrose, glutamic acid and methionine. Absorption of nutrients differed between casein and casein-potato tubers diets.

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