Asian Journal of Dairy and Food Research, volume 36 issue 2 (june 2017) : 156-160

Amino acid analysis of cocoa fermented by high performance liquid chromatography  (HPLC)

Mulono Apriyanto*, Sutardi, Supriyanto, Eni Harmayani
1<p>Department of Food Technology, Indragiri of &nbsp;Islam University, Indragiri Hilir Riau, Indonesia</p>
Cite article:- Apriyanto* Mulono, Sutardi, Supriyanto, Harmayani Eni (2017). Amino acid analysis of cocoa fermented by high performanceliquid chromatography (HPLC) . Asian Journal of Dairy and Food Research. 36(2): 156-160. doi: 10.18805/ajdfr.v36i02.7962.

The aims of the study was to improve quality of cocoa bans by fermentation of sun dried cocoa beans. The characteric fermented cocoa beans was determined by maesuring amino acids cocoa beans during fermentation. The fermentation process used 3 level treatment i.e. control (without inoculum), mixed culture of microbies added at the begining fermentation. Regulated temperature during fermentation, respectively 35 ° C (24 hours), 45 ° C (24 second clock), 55 ° C (24- hour three) and 35 ° C (last 48 hours) and then fermentation was conducted for 120 hours. The result show all cocoa beans total amino acids hydrophobic increase during fermentation from 7.21 to 7.46 ìg/g for control, 8.19 to 8.26 ìg/g for addition of inoculum at begining of fermentation and from  7.56 to 8.74 ìg/g for addition of inoculum at the begining and midle of fermentation. 

  1. Afoakwa, E. O., Budu, A. S., Mensah-brown, H., and Felix, J. (2014). Changes in biochemical and physico-chemical qualities during drying of pulp preconditioned and Fermented Cocoa (Theobroma cacao ) Beans. Internasional Food Research Journal 20(4): 1843-1853.

  2. Anonymous. (2013). Produksi Perkebunan Besar menurut Jenis Tanaman, Indonesia. Http:// Acces date 29/03/014.

  3. Ardhana, M., and Fleet, G. (2003). The microbial ecology of cocoa bean fermentations in Indonesia. International Journal of Food Microbiology, 86(1-2), 87–99.

  4. Ardianingsih, R. (2009). Penggunaan high performance liquid chromatography (HPLC) dalam proses analisa deteksi ion. Berita Dirgantara, 10(4), 101-104.

  5. Atmaja, P.I.M. (2013). Pengaruh Perlakuan Pendahuluan pada Inkubasi Biji Kakao Kering non fermntasi dalam buffer asetat terhadap ssifat sensoaris pasta cokelat gelap, Thesis Fakultas Teknologi Pertanian Universitas Gadjah mada

  6. Biehl, B., and Passern, D. (1982). Proteolysis during fermentation-like incubation of cocoa seeds. Journal of the Science of Food and Agriculture, 33,1280-1290.

  7. Biehl, B., Brunner, E., Passern, D., Quesnelh, V. C., and Adomako, D. (1985). Acidification , Proteolysis and Flavour Potential in Fermenting Cocoa Beans, 583–598.

  8. Camu, N., Winter, T. De, Addo, S. K., Takrama, J. S., Bernaert, H., and Vuyst, L. De. (2008). Fermentation of cocoa beans/ : influence of microbial activities and polyphenol concentrations on the flavour of chocolate, 2297(May), 2288–2297.    10.1002/jsfa, 

  9. Crafack, M., Hanna, K., Carl, E. E., Mikael, A. P., Sofie, S., Andreas, B., and Dennis, S. N. (2014). Impact of starter cultures and fermentation techniques on the volatile aroma and sensory profile of chocolate. Food Research International, 63,306316.

  10. de Brito, E. S., Pezoa, G., Gallao, M. I., Angelo L. C., Pedro, S. F., and Marcia, R. B. (2000). Structural and chemical changes in cocoa (Theobroma cocoa beans l.) during fermentation, drying and roasting. Journal Of    The Science Of Food And Agriculture,81,281-288.;2-B

  11. Eichner, K., Schnee, R., and Heinzler, M. (1994). Indicator compounds and precursors for cocoa aroma formation.In T. H. Parliament, M. J. Morello & R. J. McGorin (Eds.). Thermally Generated Flavour: Maillard,Microwave and Extrusion Processes (pp. 218-227). ACS Symposium Series No. 543. Washington, DC:American Chemical Society.

  12. Gu, F., Lehe, T., Huasong, W., Yimin,g F., Fei, X., Zhong, C., and Wang, Q. (2013). Comparison of cocoa beans from China, Indonesia and Papua New Guinea. Foods, 2: 183-197.

  13. Harrington, W. L. (2011). The Effects Of Roasting Time and Temperature On The Antioxidant Capacity Of Cocoa Beans From Dominican Republic, Ecuador, Haiti, Indonesia, and Ivory Coast. A Thesis Presented for the Master of Science Degree The University of Tennessee, Knoxville

  14. Jinap, S., Ikrawan, Y., Bakar, J., Sari, N., and Lioe, H. N. (2008). Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase. Journal of Food Science, 73:141-147.    j.1750-3841.2008.00858.x

  15. Jinap, S., Lioe, H. N., Yusep, I., Nazamid, S., and Jamilah, B. (2010). Role of carboxypeptidases to the free amino acid composition, methylpyrazine formation and sensory characteristic of under-fermented cocoa beans. International Food Research Journal, 17: 763-774.

  16. Misnawi, J. S., Jamilah, B., and Nazamid, S. (2004). Effect of polyphenol concentration on pyrazine formation during cocoa liquor roasting. Food Chemistry, 85:73-80.

  17. Misnawi, Sri Mulato, Sukrisno, W., Awad, S., and Sugiyono (2005). Optimasi suhu dan lama penyangraian biji kakao menggunakan penyangrai skala kecil tipe silinder. Pelita Perkebunan, 21(3): 169-183.

  18. Mulono Apriyanto, Sutardi, Supriyadi and Eni Harmayani. (2016). Study on effect of fermentation to the quality parameter of cocoa bean in Indonesia. Asian J. Dairy and Food Res., 35 (2): 2016 : 160-163

  19. Nollet, L. M. L. (1996). Handbook of Food Analysis, Amino Acid.

  20. Puziah, H., Jinap, S., Sharifah, K. S. M., and Asbi, A. (1998). Changes in free amino acids, peptide-N, sugar and pyrazine    concentration during cocoa fermentation. J. Sci. Food Agric, 78:535-542.    0010(199812)78:4%3C535::AID-JSFA151%3E3.0.CO;2-6

  21. Schwan, R. F., and Wheals, A. E. (2004). The microbiology of cocoa fermentation and its role in chocolate quality. Critical Reviews in Food Science and Nutrition, 44(4):205–221.

  22. Sulistyowati. (1988). Keasaman Biji kakao dan masalahnya. Pelita Perkebunan, 3:151-158.

  23. Sumarno, S. N., Narsito, I., and Izul, F. (2002). Estimasi kadar protein dalam bahan pangan melalui analisis nitrogen total dan analisis asam amino. Majalah Farmasi Indonesia, 13(1): 34-43

  24. Voigt, J., Biehl, B., and Kamaruddin, S. (1993).The major seed protein of Theobroma cacao L. Food Chemistry, 47:145-147. http://

  25. Voigt, J., Biehl, B., Heinrichs, H., Kamaruddin, S., Gaim, M., and Hugi, A. (1994). In-vitro formation of cocoa-specific aroma precursors: aroma-related peptides generated from cocoa-seed protein by co-operation of an aspartic endoprotease and a carboxypeptidase. Food Chemistry, 49: 173-180.

  26. Yusep, I., Jinap, S., Jamilah, B., and Nazamid, S. (2002). Influence of carboxypeptidases on free amino acid, peptide and methylpyrazine contents of under-fermented cocoa beans. J. Sci. Food Agric, 82:1584-1592.

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