Loading...

Agricultural Science Digest

​The Histopathology of Alimentary Canal of Stinging Catfish Heteropneustes fossilis (Bloch, 1794), Susceptible for Trematodes Incursion

DOI: 10.18805/ag.D-5366    | Article Id: D-5366 | Page : 233-237
Citation :- ​The Histopathology of Alimentary Canal of Stinging Catfish Heteropneustes fossilis (Bloch, 1794), Susceptible for Trematodes Incursion.Agricultural Science Digest.2022.(42):233-237
Pooja Vishwakarma, Ramakant Maurya, Anand M. Saxena poojavishwakarma19900@gmail.com
Address : Department of Zoology, Helminthology Laboratory, University of Lucknow, Lucknow-226 007, Uttar Pradesh, India.
Submitted Date : 1-05-2021
Accepted Date : 15-06-2021
First Online:

Abstract

Background: Heteropneustes fossilis (Bloch, 1794) is one of the popular freshwater fish known for its food quality and nutritional value. This study was undertaken with an aim to find out histopathological changes in the intestine of freshwater fish Heteropneustes fossilis, host of trematode parasite Masenia vittatusia Agarwal, 1963.
Methods: The intestine of uninfected fish used to determine normal anatomy, while infected intestine to determine pathological changes. The normal histological procedure was followed, which included fixing, rinsing, dehydrating and embedding of tissue. Tissue was cut in 4-6 µm thick sections using a rotary microtome. Haematoxylin and eosin stains were used.
Result: The highest damage was observed in the mucosal layer with ruptured and fused microvilli, hyperplasia of villi, damaged columnar epithelium layer and spread of loose connective tissue into the lumen. Other degenerative modifications included hypertrophy of blood vessels in mucosa and muscularis part, some of them ruptured might be the cause of haemorrhage inside the layers. Other changes include variation in the routine shape of all three layers. The mucosal layer showed aggregation of lymphocytes and mast cells as well.

Keywords

​Heteropneustes fossilis Hypertrophy Hyperplasia lymphocytes Masenia vittatusia Trematode

References

  1. Agarwal, V. (1963). On three trematodes from the intestine of a fresh water fish Mystus vittatus (Bl.) from Lucknow. Indian J. Helminth.15: 138-147.
  2. Bray, R.A., Gibson, D., Jones. (2008). A keys to the Trematoda Volume 3. CAB International and Natural History Museum, London.
  3. Bose, K.C. and Sinha, A.K. (1979). The histopathology of the stomach of the fish Channa gachua (Ham) (Channidae). Attributable to the digenetic trematode, Genarchopsis goppo (OZAKI), Hemiuuridae. Current Science. 48(16): 747-748. 
  4. Barbara, B. (1980). Pathological changes in cyprinid fry infected by Bucephalus polymorphus and Rhipidocotyle illensis metacercariae (Trematoda: Bucephalidae). Act. Parasitol. Pol. 27(15-18): 241-246. 
  5. Chatterji, R.C. (1933). On the trematode parasites of a Rangoon siluroid fish Clarias batrachus (Linneus, 1785). Bull. Acad. Sci. Allahabad. 3: 33-40.
  6. Cinar, K. and Senol, N. (2006). Histological and histochemical characterization of the mucosa of the digestive tract in flower fish (Pseudophoxinus antalyae). Anat Histol Embryol. 35: 147-151.
  7. Chung, Yui–tan. (1981). A study on the histopathology in the Wolfian ducts of Hypentelium nigricans (Osteichthyes: Catostomidae) caused by Phyllodistomum superbum (Trematoda: Gorgoderidae). Q.J. Taiwanmus (Taipeli). 34(3/4): 237- 240. 
  8. Dezfuli, B.S., Szekeli, C., Giovinazzo, G., Hills, K. and Giari, L. (2009). Inflammatory response to parasitic helminths in the digestive tract of Anguilla anguilla (L.). Aquaculture. 296: 1-6.
  9. Gupta, A.K. and Agarwal, S.M. (1984). Host-parasite relations in Channa punctatus and Euclinostomum heterostomum. Transaminase and total proteins and free aminoacids. Current Science. 53: 710-711. 
  10. Rashad, M. and Fath El-Bab. (2004). Fundamentals of the Histology of Fish, Part I Histology of Teleosts. Second edition. 
  11. Suvarna Kim S., Layton Christopher and Bancroft John D. (2018). Bancroft’s Theory and Practice of Histological Techniques. Elsevier, 8th Edition.
  12. Matos, L.V., Oliveira, M.I.B., Gomes, A.L.S. and Silva, G.S. (2017). Morphological and histochemical changes associated with massive infection by Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae) in the farmed freshwater fish Colossoma macropomum Cuvier, 1818 from the Amazon State, Brazil. Parasitology Research. 116(3): 1029-1037.
  13. Reddy, B.L. and Benarjee G. (2013). Intestinal histopathology of trematode infected fish, Channa straitus. Biolife:  An International Journal of Biology and Life Sciences. 1(1): 29-31.
  14. Ross, M.H. and Pawlina, W. (2016). Histology: A text and atlas with correlated cell and molecular biology. Philadelphia: Lippincott Williams and Wilkins. ISBN 978-85-277-2964-2. 7th Edition.
  15. Saha, K.C. and Guha, B.C. (1939). Nutritional investigation on Bengal fish. Indian Journal of Medical Research. 26: 921- 927.
  16. Srivastava, G.J. (2002). Fishes of Uttar Pradesh and Bihar. Vishwavidyalaya Prakashan, Varanasi, India, pp. 1-203. 9th Ed.
  17. Sanil, N.K., Asokan, P.K., John, L. and Vijayan, K.K. (2010). Pathological manifestations of the acanthocephalan parasite, Tenuiproboscis sp. in the mangrove red snapper (Lutjanus argentimaculatus, Forsskål, 1775), a candidate species for aquaculture from Southern India. Aquaculture. 310(3-4): 259-266.
  18. Shahin, M.I.H., Chandra, K.J. Das, D.R. and Khalil, S.M.I. (2013). Morphology and histopathology of alimentary canal of Clarias batrachus (Linnaeus) and Heteropneustes fossilis (Bloch). International Research Journal of Applied Life Sciences. 2(03): 11-20.
  19. Yamaguti, S. (1954). Helminthum. Vol 1. The Digenetic Trematodes of fishes. Published by author. Tokyo (1953).

Global Footprints