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Research Article

volume 41 special issue (april 2021) : 207-210,   Doi: 10.18805/ag.D-5165

Evaluation of Optimal Doses for Gamma Rays and Sodium Azide in Linseed Genotypes

R
Roshan Jahan
S
Saima Malik
S
Shazia Bi Ansari
S
Samiullah Khan
1Department of Botany, Aligarh Muslim University, Aligarh-202 002, Uttar Pradesh, India.
Cite article:- Jahan Roshan, Malik Saima, Ansari Bi Shazia, Khan Samiullah (2020). Evaluation of Optimal Doses for Gamma Rays and Sodium Azide in Linseed Genotypes. Agricultural Science Digest. 41(2021): 207-210. doi: 10.18805/ag.D-5165.

ABSTRACT

Background: Linseed is one of the most important medicinal plants grown for its various health benefits. The seeds of linseed contain a good and essential fatty acid profile that is omega-3 fatty acid/Alpha linolenic acid. It helps in the prevention of various disease including inflammation, cardiovascular problems, cancer, diabetes etc. Induced mutagenesis is an easy and cost effective technique to induce desired genetic variability, which either does not occur naturally or is not accessible to plant breeders. Genetic variability is enhanced by the influence of various chemical or physical mutagens. The usefulness of any mutagen relies not only on its efficiency but also on its effectiveness. 
Methods: Dry and healthy seeds of linseed (var. Padmini and IC0096650) were treated with different doses of gamma rays and sodium azide. The experiment was conducted during Rabi season of November 2016-March 2017. The selection of optimum doses of mutagens through the determination of LD50 values has been calculated on the basis of the seed germination as well as plant survival. 
Result: The present investigation reveals genotypic response of two linseed varieties towards different doses of gamma rays and sodium azide. Variety IC0096650 exhibited higher degree of sensitivity than variety Padmini with respect to the mutagens used. Results showed that 200Gy dose of gamma rays and 0.4% dose of sodium azide was the maximum non-lethal strength of the respective mutagen for the induction of the mutation in linseed genotypes.

REFERENCES

  1. Ananthaswamy, H.N., Vakil, U.K., Srinivasan, A. (1971). Biochemical and physiological changes in gamma irradiated wheat during germination. Radiation Botany. 11: 1-12. 
  2. Bernacchia, R., Preti, R., Vinci, G. (2014). Chemical composition and health benefits of flaxseed. Austin Journal of Nutrition and Food Sciences. 2(8): 1045.
  3. Bhatty, R.S. and Cherdkiatgumchai, P. (1990). Compositional analysis of laboratory prepared and commercial samples of linseed meal and of hull isolated from flax. Journal of the American Oil Chemists’ Society. 67(2): 79-84.
  4. Chauhan, Y.S. and Singh, R.P. (1975). Morphological studies in safflower (Carthamus tinctorius Linn.) with special reference to the effect of 2,4-D and gamma rays I. Vegetative shoot apex. Radiation Botany. 15: 69-77.
  5. Chrispeels, M.J. and Varner, J.E. (1967). Gibberellic acid induced synthesis and release of Lamylase and ribonuclease by isolated barley aleurone layers. Plant Physiology. 42: 396-406.
  6. Gaul, H. (1964). Mutations in plant breeding. Radiation Botany. 4: 155-232.
  7. Goyal. A., Sharma, V., Upadhyay, N., Gill, S., Sihag, M. (2014). Flax and flaxseed oil: an ancient medicine and modern functional food. Journal of food science and technology. 51(9): 1633-1653.
  8. Hevesy, G. (1945). On the effect of roentgen rays on cellular division. Review of Modern Physics. 17: 102. 
  9. Khan, S., Wani, M.R. and Parveen, K. (2006). Sodium azide induced high yielding early mutant in lentil. Agricultural Science Digest. 26: 65-66.
  10. Khursheed, S., Laskar, R.A., Raina, A., Amin, R., Khan, S. (2015). Comparative analysis of cytological abnormalities induced in Vicia faba L. genotypes using physical and chemical mutagenesis. Chromosome Science. 18(3-4): 47-51.
  11. Kurobane, I.H., Yamaguchi, H., Sander, C., Nilan, R.A. (1979). The effects of gamma irradiation on the production and secretion of enzymes and enzymatic activities in barley. Environmental and Experimental Botany. 19: 75-84.
  12. Laskar, R. A., Amin, R., Khan, S. (2017). Evaluation of Optimal 
  13. Doses for Gamma Rays and Hydrazine Hydrates in Lentil Genotypes. Trends in Bioscience. 10(27).
  14. Laskar, R.A., Chaudhary, C., Khan, S., Chandra, A. (2018). Induction of mutagenized tomato populations for investigation on agronomic traits and mutant phenotyping. Journal of the Saudi society of agricultural sciences. 17(1): 51-60. 
  15. Laskar, R.A., Khan, S., Khursheed, S., Raina, A., Amin, R. (2015). Quantitative analysis of induced phenotypic diversity in chickpea using physical and chemical mutagenesis. Journal of Agronomy. 14: 102.
  16. Laskar, R.A., Laskar, A.A., Raina, A., Khan, S., Younus, H. (2018). Induced mutation analysis with biochemical and molecular characterization of high yielding lentil mutant lines. International Journal of Biological Macromolecules. 109: 167-179. 
  17. Mutant Varieties Database, IAEA/FAO, 2019.
  18. Novak, F. J. and Brunner, H. (1992). Plant breeding Induced mutation technology for crop improvement. IAEA Bull. 4: 25-33.
  19. Raina, A., Khursheed, S., Khan, S. (2018). Optimisation of mutagen doses for gamma rays and sodium azide in Cowpea genotypes. Trends in Biosciences. 11(13): 2386-2389.
  20. Raina, A., Laskar, R.A., Khursheed, S., Khan, S., Parveen, K., Amin, R., Khan, S. (2017). Induce physical and chemical mutagenesis for improvement of yield attributing traits and their correlation analysis in chickpea. International Letters of Natural Sciences. 61: 14-22.
  21. Ramchander, S., Ushakumari, R., Pillai, M.A. (2015). Lethal dose fixation and sensitivity of rice varieties to gamma radiation. Indian Journal of Agricultural Research. 49(1): 24-31.
  22. Sood, S., Jambulkar, S.J., Sood, A., Gupta, N., Kumar, R., Singh, Y. (2016). Median lethal dose estimation of gamma rays and ethyl methane sulphonate in bell Pepper (Capsicum Annuum L.). Sabrao Journal of Breeding and Genetics. 48 (4): 528-535. 
  23. Usuf, K. K., Nair and P. M. (1974). Effect of gamma irradiation on the indole acetic acid synthesizing system and its significance in sprout inhibition of potatoes. Radiation Botany. 14: 251- 256.
  24. Wani, M.R. (2019). Comparative biological sensitivity and mutability of chemo-mutagens in lentil (Lens culinaris Medik). Legume Research. 0976-0571 (1-6). 
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    Research Article

    volume 41 special issue (april 2021) : 207-210,   Doi: 10.18805/ag.D-5165

    Evaluation of Optimal Doses for Gamma Rays and Sodium Azide in Linseed Genotypes

    R
    Roshan Jahan
    S
    Saima Malik
    S
    Shazia Bi Ansari
    S
    Samiullah Khan
    1Department of Botany, Aligarh Muslim University, Aligarh-202 002, Uttar Pradesh, India.
    Cite article:- Jahan Roshan, Malik Saima, Ansari Bi Shazia, Khan Samiullah (2020). Evaluation of Optimal Doses for Gamma Rays and Sodium Azide in Linseed Genotypes. Agricultural Science Digest. 41(2021): 207-210. doi: 10.18805/ag.D-5165.

    ABSTRACT

    Background: Linseed is one of the most important medicinal plants grown for its various health benefits. The seeds of linseed contain a good and essential fatty acid profile that is omega-3 fatty acid/Alpha linolenic acid. It helps in the prevention of various disease including inflammation, cardiovascular problems, cancer, diabetes etc. Induced mutagenesis is an easy and cost effective technique to induce desired genetic variability, which either does not occur naturally or is not accessible to plant breeders. Genetic variability is enhanced by the influence of various chemical or physical mutagens. The usefulness of any mutagen relies not only on its efficiency but also on its effectiveness. 
    Methods: Dry and healthy seeds of linseed (var. Padmini and IC0096650) were treated with different doses of gamma rays and sodium azide. The experiment was conducted during Rabi season of November 2016-March 2017. The selection of optimum doses of mutagens through the determination of LD50 values has been calculated on the basis of the seed germination as well as plant survival. 
    Result: The present investigation reveals genotypic response of two linseed varieties towards different doses of gamma rays and sodium azide. Variety IC0096650 exhibited higher degree of sensitivity than variety Padmini with respect to the mutagens used. Results showed that 200Gy dose of gamma rays and 0.4% dose of sodium azide was the maximum non-lethal strength of the respective mutagen for the induction of the mutation in linseed genotypes.

    REFERENCES

    1. Ananthaswamy, H.N., Vakil, U.K., Srinivasan, A. (1971). Biochemical and physiological changes in gamma irradiated wheat during germination. Radiation Botany. 11: 1-12. 
    2. Bernacchia, R., Preti, R., Vinci, G. (2014). Chemical composition and health benefits of flaxseed. Austin Journal of Nutrition and Food Sciences. 2(8): 1045.
    3. Bhatty, R.S. and Cherdkiatgumchai, P. (1990). Compositional analysis of laboratory prepared and commercial samples of linseed meal and of hull isolated from flax. Journal of the American Oil Chemists’ Society. 67(2): 79-84.
    4. Chauhan, Y.S. and Singh, R.P. (1975). Morphological studies in safflower (Carthamus tinctorius Linn.) with special reference to the effect of 2,4-D and gamma rays I. Vegetative shoot apex. Radiation Botany. 15: 69-77.
    5. Chrispeels, M.J. and Varner, J.E. (1967). Gibberellic acid induced synthesis and release of Lamylase and ribonuclease by isolated barley aleurone layers. Plant Physiology. 42: 396-406.
    6. Gaul, H. (1964). Mutations in plant breeding. Radiation Botany. 4: 155-232.
    7. Goyal. A., Sharma, V., Upadhyay, N., Gill, S., Sihag, M. (2014). Flax and flaxseed oil: an ancient medicine and modern functional food. Journal of food science and technology. 51(9): 1633-1653.
    8. Hevesy, G. (1945). On the effect of roentgen rays on cellular division. Review of Modern Physics. 17: 102. 
    9. Khan, S., Wani, M.R. and Parveen, K. (2006). Sodium azide induced high yielding early mutant in lentil. Agricultural Science Digest. 26: 65-66.
    10. Khursheed, S., Laskar, R.A., Raina, A., Amin, R., Khan, S. (2015). Comparative analysis of cytological abnormalities induced in Vicia faba L. genotypes using physical and chemical mutagenesis. Chromosome Science. 18(3-4): 47-51.
    11. Kurobane, I.H., Yamaguchi, H., Sander, C., Nilan, R.A. (1979). The effects of gamma irradiation on the production and secretion of enzymes and enzymatic activities in barley. Environmental and Experimental Botany. 19: 75-84.
    12. Laskar, R. A., Amin, R., Khan, S. (2017). Evaluation of Optimal 
    13. Doses for Gamma Rays and Hydrazine Hydrates in Lentil Genotypes. Trends in Bioscience. 10(27).
    14. Laskar, R.A., Chaudhary, C., Khan, S., Chandra, A. (2018). Induction of mutagenized tomato populations for investigation on agronomic traits and mutant phenotyping. Journal of the Saudi society of agricultural sciences. 17(1): 51-60. 
    15. Laskar, R.A., Khan, S., Khursheed, S., Raina, A., Amin, R. (2015). Quantitative analysis of induced phenotypic diversity in chickpea using physical and chemical mutagenesis. Journal of Agronomy. 14: 102.
    16. Laskar, R.A., Laskar, A.A., Raina, A., Khan, S., Younus, H. (2018). Induced mutation analysis with biochemical and molecular characterization of high yielding lentil mutant lines. International Journal of Biological Macromolecules. 109: 167-179. 
    17. Mutant Varieties Database, IAEA/FAO, 2019.
    18. Novak, F. J. and Brunner, H. (1992). Plant breeding Induced mutation technology for crop improvement. IAEA Bull. 4: 25-33.
    19. Raina, A., Khursheed, S., Khan, S. (2018). Optimisation of mutagen doses for gamma rays and sodium azide in Cowpea genotypes. Trends in Biosciences. 11(13): 2386-2389.
    20. Raina, A., Laskar, R.A., Khursheed, S., Khan, S., Parveen, K., Amin, R., Khan, S. (2017). Induce physical and chemical mutagenesis for improvement of yield attributing traits and their correlation analysis in chickpea. International Letters of Natural Sciences. 61: 14-22.
    21. Ramchander, S., Ushakumari, R., Pillai, M.A. (2015). Lethal dose fixation and sensitivity of rice varieties to gamma radiation. Indian Journal of Agricultural Research. 49(1): 24-31.
    22. Sood, S., Jambulkar, S.J., Sood, A., Gupta, N., Kumar, R., Singh, Y. (2016). Median lethal dose estimation of gamma rays and ethyl methane sulphonate in bell Pepper (Capsicum Annuum L.). Sabrao Journal of Breeding and Genetics. 48 (4): 528-535. 
    23. Usuf, K. K., Nair and P. M. (1974). Effect of gamma irradiation on the indole acetic acid synthesizing system and its significance in sprout inhibition of potatoes. Radiation Botany. 14: 251- 256.
    24. Wani, M.R. (2019). Comparative biological sensitivity and mutability of chemo-mutagens in lentil (Lens culinaris Medik). Legume Research. 0976-0571 (1-6). 
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