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Research Article

volume 40 issue 3 (september 2020) : 280-284,   Doi: 10.18805/ag.D-5099

Comparison of Yield, Nutrient Content and Antibacterial Activities of Wild and Cultivated Isolates of Pleurotus djamor

A
Anjana Sharma
S
Savita Jandaik
N
Neetika Thakur
1Department of Plant Pathology, Dr. YS Parmar University of Horticulture and Forestry, Nauni-173 230, Solan, Himachal Pradesh, India. 
Cite article:- Sharma Anjana, Jandaik Savita, Thakur Neetika (2020). Comparison of Yield, Nutrient Content and Antibacterial Activities of Wild and Cultivated Isolates of Pleurotus djamor. Agricultural Science Digest. 40(3): 280-284. doi: 10.18805/ag.D-5099.

ABSTRACT

Background: Pleurotus species constitutes one of the choicest edible mushrooms, it is commonly known as “Oyster Mushroom” and in India it is commonly called as “Dhingri. It has important medicinal, biotechnological properties and environmental applications. Its cultivation can be done on a number of agricultural wastes and organic waste materials. There are significant problems in classifying Pleurotus isolates using only morphological characters which are often unreliable and inconclusive mainly due to the large influence exerted by environmental factors. 
Methods: A wild isolate of Pleurotus was collected from North western Himalayas and its identity was confirmed by molecular studies. Yield, nutritional components and its bioactive molecules were compared with the cultivated isolate of Pleurotus djamore Antibacterial activities of both the isolates of were performed  against Escherichia coli and Staphylococcus aureus by disc diffusion. 
Result: Molecular studies confirmed the identity of wild isolate of Pleurotus as Pleurotus djamore. The maximum yield of wild isolate was on paddy straw followed by wheat straw. The protein content was 32.3±0.50g-1 in wild isolate and 28.7±1.67g-1 in cultivated isolate. Presence of nutritional components and phytochemicals inferred in this study indicate the importance of Pleurotus djamore in the pharmaceutical.

REFERENCES

  1. Adebayo, E.A. and Ishola, O.R. (2009). Phytochemical and anti- -microbial screening of crude extracts of Terminalia gaucescens. African Journal of Pharmacy and Pharmacology. 3(5): 217-221.
  2. Adejoye, O.D., Adebayo-Tayo, B.C, Ogunjobi, A.A., Olaoye, O.A, Fadahunsi, F.I. (2006). African Journal of Biotechnology. 5: 1355-1359.
  3. Almey, A.A., Khan, A.J.C., Zahir, S.I., Suleiman, M.K., Aisyah, M.R., Rahim, K.K. (2010). Total phenolic content and primary antioxidant activity of methanolic extracts of aromatic plants’ leaves. International Food Research Journal. 17: 1077-1084.
  4. Anderson, D.L., Gibbs, A.J., Gibson, N.L. (1984). Identification and phylogeny of spore-cyst fungi (Ascosphaera species) using ribosomal DNA sequences. Mycological Research. 102: 541-547.
  5. AOAC. (1984). Official methods of analysis 14th Eds Association of Official Agricultural Chemists In: Williams, S (Ed) AOAC Arlington, CA 1984.
  6. Bauer, A.W., Kirby, W.M., Sherris, J.C., Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 45: 493-6.
  7. Bernardo, E.L., Jorge, E.W., Edgardo, A. (2004). The genus Pleurotus in Argentina. Mycologia. 96: 845–858.
  8. Chang, S.T. and Miles, P.G. (1992). Mushrooms biology a new discipline Mycologist. 6: 64-65. 
  9. Dehariya, P. and Vyas, D. (2015). Evaluation of different spawns and substrates on growth and yield of Pleurotus sajor- caju. International Journal of Recent Scientific Research. 6(3): 2908-2911.
  10. Dung, N.T.P., Tuyen, D.B., Quang, P.H. (2012). Morphological and genetic characteristics of Oyster mushrooms and conditions effecting on its spawn growing. Int Food Res J. 19: 347-352.
  11. Gowda, N.A.N. and Manvi, D. (2019). Agro-residues Disinfection Methods for Mushroom Cultivation. Agri Revs. 40(2): 93-103.
  12. Harborne, J.B. (1998). Phytochemical methods A Guide to modern techniques of plant analysis 3rd ed, Chapman and Hall Int, New York.
  13. Hossain Mijan, M.D. (2017). Effect of Different Substrates on Growth and Yield of Oyster Mushroom (Pleurotus sajorcaju). Int. J. Curr. Microbiol. App. Sci. 6(12): 760-764.
  14. Kamra, A. and Bhatt, A.B. (2012). Evaluation of antimicrobial and antioxidant activity of Ganoderma lucidum extracts against human pathogenic bacteria. Inter J Pharm Sci. 4(2): 359-361.
  15. Khare, K.B., Mutuku, J.M., Achwania, O.S., Otaye, D.O. (2010b). Effect of mycelium carriers, amount of additives on spawn quality and yield of Pleurotus sajor- caju and P. florida on wheat straw substrate sterilized using various methods. Bots. J. Agric. Appl. Sci. 6 (2): 13 - 21.
  16. Lindequist, U., Niedermeyer, T.H.J., Julich, W. (2005). The pharmacological potentials of mushrooms. International Journal Evidence-Based Complementary and Alternative Medicine. 2: 285-299.
  17. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with the Folin-Phenol reagent. Journal of Biological Chemistry. 193: 265-27.
  18. Moncalvo, J.M., Wang, H.H., Hseu, R.S. (1995). Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia. 87: 223-238.
  19. Nehra, K., Meenakshi, Kumar, M., Yadav, A. (2012). Evaluation of antimicrobial potential of fruiting body extracts of Pleurotus ostreatus (oyster mushroom). International J Microbial Resource Technol. 4: 391-400 
  20. Okwulehie, I.C. and Ogoke, J.A. (2013). Bioactive, nutritional and heavy metal constituents of some edible mushrooms found in Abia State of Nigeria. International Journal of Applied Microbiology and Biotechnology Research. 1: 7-15.
  21. Oyenuga, V.A. (1968) Nigeria’s foods and feedong stuffs Ibadan University press, 1-90.
  22. Pala, S.A., Wani, A.H., Mir, R.A. (2012). Yield performance of Pleurotus sajorcaju on different agro-based wastes. Ann Biol Res. 3(4): 1938-1941.
  23. Park, Y.J., Kwon, O.C., Son, E.S., Yoon, D.E., Han, W., Yoo, Y.B. (2012). Taxonomy of Ganoderma lucidum from Korea based on rDNA and Partial â-Tubulin gene sequence analysis. Mycobiology. 40: 71-75.
  24. Ravat, H. and John, P. (2016). Macrofungal diversity in Dangs district, Gujarat, India. I.J.S.N. 7: 477-482.
  25. Sharma, A., Thakur, N., Jandaik, S., Singh, R (2019). Cultivation of wild Pleurotus species on agricultural substrates and evaluation of their nutritional properties. Int J Adv Res. 6(12): 1425-1429.
  26. Singh, S., Singh, G., Rahul, S.N., Kuma, A., Bhanu, P., Bankoti, P., Pandey, R.K. (2017). Effect of different substrates on the growth and yield of oyster mushrooms (Pleurotus djamor). International Journal of Agriculture Sciences. 9(4): 3721-3723.
  27. Syed, A.A., Kadam, J.A., Mane, V.P., Patil, S.S, Baig, M.M.V. (2009). Biological efficiency and nutritional contents of Pleurotus florida (Mont) Singer cultivated on different agro-wastes. Nature and Science. 7(1): 44-48.
  28. Tupatkar, P.N. and Jadhao, S.M. (2006). Effects of different substrates on yield of oyster mushroom (Pleurotus sajor - caju). Agricultural Science Digest. (26): 224-226.
  29. Van Kan, J.A.L., Van den Ackerveken, G.F.J.M., De Wit, P.J.G.M. (1991). Cloning and characterization of cDNA of avirulence gene Avr9 of the fungal tomato pathogen Cladosporium fulvum, causal agent of tomato leaf mold. MOI. Plant-    Microbe Interact. 52-59.
  30. Yadav, M.K. (2015). Studies on Biodiversity of Edible Mushrooms from Vindhya forest PhD thesis Banaras Hindu University Varanasi, pp110.
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    Research Article

    volume 40 issue 3 (september 2020) : 280-284,   Doi: 10.18805/ag.D-5099

    Comparison of Yield, Nutrient Content and Antibacterial Activities of Wild and Cultivated Isolates of Pleurotus djamor

    A
    Anjana Sharma
    S
    Savita Jandaik
    N
    Neetika Thakur
    1Department of Plant Pathology, Dr. YS Parmar University of Horticulture and Forestry, Nauni-173 230, Solan, Himachal Pradesh, India. 
    Cite article:- Sharma Anjana, Jandaik Savita, Thakur Neetika (2020). Comparison of Yield, Nutrient Content and Antibacterial Activities of Wild and Cultivated Isolates of Pleurotus djamor. Agricultural Science Digest. 40(3): 280-284. doi: 10.18805/ag.D-5099.

    ABSTRACT

    Background: Pleurotus species constitutes one of the choicest edible mushrooms, it is commonly known as “Oyster Mushroom” and in India it is commonly called as “Dhingri. It has important medicinal, biotechnological properties and environmental applications. Its cultivation can be done on a number of agricultural wastes and organic waste materials. There are significant problems in classifying Pleurotus isolates using only morphological characters which are often unreliable and inconclusive mainly due to the large influence exerted by environmental factors. 
    Methods: A wild isolate of Pleurotus was collected from North western Himalayas and its identity was confirmed by molecular studies. Yield, nutritional components and its bioactive molecules were compared with the cultivated isolate of Pleurotus djamore Antibacterial activities of both the isolates of were performed  against Escherichia coli and Staphylococcus aureus by disc diffusion. 
    Result: Molecular studies confirmed the identity of wild isolate of Pleurotus as Pleurotus djamore. The maximum yield of wild isolate was on paddy straw followed by wheat straw. The protein content was 32.3±0.50g-1 in wild isolate and 28.7±1.67g-1 in cultivated isolate. Presence of nutritional components and phytochemicals inferred in this study indicate the importance of Pleurotus djamore in the pharmaceutical.

    REFERENCES

    1. Adebayo, E.A. and Ishola, O.R. (2009). Phytochemical and anti- -microbial screening of crude extracts of Terminalia gaucescens. African Journal of Pharmacy and Pharmacology. 3(5): 217-221.
    2. Adejoye, O.D., Adebayo-Tayo, B.C, Ogunjobi, A.A., Olaoye, O.A, Fadahunsi, F.I. (2006). African Journal of Biotechnology. 5: 1355-1359.
    3. Almey, A.A., Khan, A.J.C., Zahir, S.I., Suleiman, M.K., Aisyah, M.R., Rahim, K.K. (2010). Total phenolic content and primary antioxidant activity of methanolic extracts of aromatic plants’ leaves. International Food Research Journal. 17: 1077-1084.
    4. Anderson, D.L., Gibbs, A.J., Gibson, N.L. (1984). Identification and phylogeny of spore-cyst fungi (Ascosphaera species) using ribosomal DNA sequences. Mycological Research. 102: 541-547.
    5. AOAC. (1984). Official methods of analysis 14th Eds Association of Official Agricultural Chemists In: Williams, S (Ed) AOAC Arlington, CA 1984.
    6. Bauer, A.W., Kirby, W.M., Sherris, J.C., Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 45: 493-6.
    7. Bernardo, E.L., Jorge, E.W., Edgardo, A. (2004). The genus Pleurotus in Argentina. Mycologia. 96: 845–858.
    8. Chang, S.T. and Miles, P.G. (1992). Mushrooms biology a new discipline Mycologist. 6: 64-65. 
    9. Dehariya, P. and Vyas, D. (2015). Evaluation of different spawns and substrates on growth and yield of Pleurotus sajor- caju. International Journal of Recent Scientific Research. 6(3): 2908-2911.
    10. Dung, N.T.P., Tuyen, D.B., Quang, P.H. (2012). Morphological and genetic characteristics of Oyster mushrooms and conditions effecting on its spawn growing. Int Food Res J. 19: 347-352.
    11. Gowda, N.A.N. and Manvi, D. (2019). Agro-residues Disinfection Methods for Mushroom Cultivation. Agri Revs. 40(2): 93-103.
    12. Harborne, J.B. (1998). Phytochemical methods A Guide to modern techniques of plant analysis 3rd ed, Chapman and Hall Int, New York.
    13. Hossain Mijan, M.D. (2017). Effect of Different Substrates on Growth and Yield of Oyster Mushroom (Pleurotus sajorcaju). Int. J. Curr. Microbiol. App. Sci. 6(12): 760-764.
    14. Kamra, A. and Bhatt, A.B. (2012). Evaluation of antimicrobial and antioxidant activity of Ganoderma lucidum extracts against human pathogenic bacteria. Inter J Pharm Sci. 4(2): 359-361.
    15. Khare, K.B., Mutuku, J.M., Achwania, O.S., Otaye, D.O. (2010b). Effect of mycelium carriers, amount of additives on spawn quality and yield of Pleurotus sajor- caju and P. florida on wheat straw substrate sterilized using various methods. Bots. J. Agric. Appl. Sci. 6 (2): 13 - 21.
    16. Lindequist, U., Niedermeyer, T.H.J., Julich, W. (2005). The pharmacological potentials of mushrooms. International Journal Evidence-Based Complementary and Alternative Medicine. 2: 285-299.
    17. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with the Folin-Phenol reagent. Journal of Biological Chemistry. 193: 265-27.
    18. Moncalvo, J.M., Wang, H.H., Hseu, R.S. (1995). Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia. 87: 223-238.
    19. Nehra, K., Meenakshi, Kumar, M., Yadav, A. (2012). Evaluation of antimicrobial potential of fruiting body extracts of Pleurotus ostreatus (oyster mushroom). International J Microbial Resource Technol. 4: 391-400 
    20. Okwulehie, I.C. and Ogoke, J.A. (2013). Bioactive, nutritional and heavy metal constituents of some edible mushrooms found in Abia State of Nigeria. International Journal of Applied Microbiology and Biotechnology Research. 1: 7-15.
    21. Oyenuga, V.A. (1968) Nigeria’s foods and feedong stuffs Ibadan University press, 1-90.
    22. Pala, S.A., Wani, A.H., Mir, R.A. (2012). Yield performance of Pleurotus sajorcaju on different agro-based wastes. Ann Biol Res. 3(4): 1938-1941.
    23. Park, Y.J., Kwon, O.C., Son, E.S., Yoon, D.E., Han, W., Yoo, Y.B. (2012). Taxonomy of Ganoderma lucidum from Korea based on rDNA and Partial â-Tubulin gene sequence analysis. Mycobiology. 40: 71-75.
    24. Ravat, H. and John, P. (2016). Macrofungal diversity in Dangs district, Gujarat, India. I.J.S.N. 7: 477-482.
    25. Sharma, A., Thakur, N., Jandaik, S., Singh, R (2019). Cultivation of wild Pleurotus species on agricultural substrates and evaluation of their nutritional properties. Int J Adv Res. 6(12): 1425-1429.
    26. Singh, S., Singh, G., Rahul, S.N., Kuma, A., Bhanu, P., Bankoti, P., Pandey, R.K. (2017). Effect of different substrates on the growth and yield of oyster mushrooms (Pleurotus djamor). International Journal of Agriculture Sciences. 9(4): 3721-3723.
    27. Syed, A.A., Kadam, J.A., Mane, V.P., Patil, S.S, Baig, M.M.V. (2009). Biological efficiency and nutritional contents of Pleurotus florida (Mont) Singer cultivated on different agro-wastes. Nature and Science. 7(1): 44-48.
    28. Tupatkar, P.N. and Jadhao, S.M. (2006). Effects of different substrates on yield of oyster mushroom (Pleurotus sajor - caju). Agricultural Science Digest. (26): 224-226.
    29. Van Kan, J.A.L., Van den Ackerveken, G.F.J.M., De Wit, P.J.G.M. (1991). Cloning and characterization of cDNA of avirulence gene Avr9 of the fungal tomato pathogen Cladosporium fulvum, causal agent of tomato leaf mold. MOI. Plant-    Microbe Interact. 52-59.
    30. Yadav, M.K. (2015). Studies on Biodiversity of Edible Mushrooms from Vindhya forest PhD thesis Banaras Hindu University Varanasi, pp110.
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