Agricultural Science Digest

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Agricultural Science Digest, volume 40 issue 4 (december 2020) : 436-439

Genetic Polymorphism in a Selective Intron of Ovine Myostatin Gene and Its Putative Relation with Carcass Traits in Sheep

D.V. Praneeth, R. Vinoo, K. Sudhakar, S. Jagadeswararao, M. Metta
1Department of Animal Genetics and Breeding, College of Veterinary Science, Sri Venkateswara Veterinary University, Garividi-535 101 Andhra Pradesh, India.
Cite article:- Praneeth D.V., Vinoo R., Sudhakar K., Jagadeswararao S., Metta M. (2022). Genetic Polymorphism in a Selective Intron of Ovine Myostatin Gene and Its Putative Relation with Carcass Traits in Sheep. Agricultural Science Digest. 40(4): 436-439. doi: 10.18805/ag.D-5081.
The main aim of the present study is to identify a suitable polymorphic locus in the myostatin gene of sheep that could be associated with production in local sheep genetic groups of Andhra Pradesh province in India. Representative samples from three local genetic groups were used in the present study namely Nellore Jodipi, Nellore Brown and Macherla Brown. Two PCR-RFLP based SNP markers located in GDF-8 (myostatin-MYST) locus were used in the present study. A PCR-RFLP assay was developed for a SNP located in the intron1 (rs119102825) of the myostatin gene. The second marker is located in exon3 which was obtained from previous studies. The SNP located in Intron1 is polymorphic and the SNP located in exon3 is monomorphic. The polymorphism information content (PIC) of the SNP in intron1 is 0.37. Association studies with limited data showed lack of association of genotypes with body weight at different ages. However, based on bioinformatic prediction, it is likely that the SNP in the intron1 locus may be involved in meat quality determination as it is close to the donor site of intron and the variation has potentiality for enhancement or repression of the gene expression. Further association studies with meat quality traits would help in understanding functional implications of the polymorphism.
  1. Anonymous. (2017). Basic Animal Husbandry and Fisheries Statistics., Government of India, New Delhi. AHS Series: 58-59.
  2. Arthur, P.F., Makarechian M. and Price M.A. (1988). Incidence of Dystocia and Perinatal Calf Mortality Resulting from Reciprocal Crossing of Double-muscled and Normal Cattle. Canadian Veterinary Journal. 29: 163-7.
  3. Boman, I.A., Klemetsdal G., Nafstad O., Blichfeldt T. and Vage D.I. (2010). Impact of two myostatin (MSTN) mutations on weight gain and lamb carcass classification in Norwegian White Sheep (Ovis aries). Genetics Selection Evolution. 42: 4.
  4. Clop, A., Marcq F., Takeda H., Pirottin D., Tordoir X., Bibe B., Bouix J., Caiment F., Elsen J.M., Eychenne F., et al. (2006). A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nature Genetics. 38: 813-8.
  5. Dimitrova, I., Bozhilova-Sakova M., Stancheva N. and Tzonev T. (2016). Molecular Analysis of Ovine Myostatin gene (MSTN) in Northeast Bulgarian Merino Sheep Breed Using PCR-RFLP. Bulgarian Journal of Agricultural Science. 22: 315–17.
  6. Elkorshy, N., Mahrous K.F. and Salem L.M. (2013). Genetic Poly- -morphism Detection in Four Genes in Egyptian and Saudi Sheep Breeds. World Applied Sciences Journal. 27: 33-43.
  7. Georgieva, S., Hristova D., Dimitrova I., Stancheva N. and Bozhilova -Sakova M. (2015). Molecular analysis of ovine calpastatin (CAST) and myostatin (MSTN) genes in Synthetic Population Bulgarian Milk sheep using PCR-RFLP Journal of Bioscience and Biotechnology. 4: 95-99.
  8. Hickford, J.G., Forrest R.H., Zhou H., Fang Q., Han J., Frampton C.M. and Horrell A.L. (2010). Polymorphisms in the ovine myostatin gene (MSTN) and their association with growth and carcass traits in New Zealand Romney sheep. Animal Genetics. 41: 64-72.
  9. McPherron, A.C., Lawler A.M. and Lee S.J. (1997). Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature Genetics. 37: 83-90.
  10. Ota, M., Fukushima H., Kulski J.K. and Inoko H. (2007). Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism. Nature Protocols. 2: 2857-64.
  11. Sambrook, J. and Russell D.W. (2001). Molecular Cloning: A laboratory Manual. New York: Cold Spring Harbor Laboratory Press.
  12. Sjakste, T., Paramonova N., Grislis Z., Trapina I. and Kairisa D. (2011). Analysis of the single-nucleotide polymorphism in the 5’UTR and part of intron I of the sheep MSTN gene. DNA Cell Biology. 30: 433-44.
  13. Sullivan, L.M., Weinberg J. and Keaney J.F., Jr. (2016). Common Statistical Pitfalls in Basic Science Research. J Am Heart Assoc. 5.
  14. Trukhachev, V., Belyaev V., Kvochko A., Kulichenko A., Kovalev D., Pisarenko S., Volynkina A., Selionova M., Aybazov M., Shumaenko S., et al. (2015). Myostatin gene (MSTN) polymorphism with a negative effect on meat productivity in Dzhalginsky Merino sheep breed. Journal of Biscience and Biotechnology. 4: 191-99.
  15. Untergasser, A., Cutcutache I., Koressaar T., Ye J., Faircloth B.C., Remm M. and Rozen S.G. (2012). Primer3-new capabilities and interfaces. Nucleic Acids Research. 40: e115.

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