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Research Article

volume 39 issue 3 (september 2019) : 254-256,   Doi: 10.18805/ag.D-4929

Real-time Expression of Stemness and Self-Renewal Genes in Canine Hematopoietic Progenitor Cells

S
S. Nandhini
G
Gowri A. Mangala
G
G.R. Baranidharan
T
T.V. Meenambigai
1Department of Animal Biotechnology, CSCRRM, Madras Veterinary College, Chennai-7,India
Cite article:- Nandhini S., Mangala A. Gowri, Baranidharan G.R., Meenambigai T.V. (2019). Real-time Expression of Stemness and Self-Renewal Genes in Canine Hematopoietic Progenitor Cells. Agricultural Science Digest. 39(3): 254-256. doi: 10.18805/ag.D-4929.

ABSTRACT

Blood cells are responsible for constant maintenance and immune protection of every cell type of the body and this relentless and brutal work requires cells that have the greatest powers of self-renewal and are designated as Hematopoietic progenitor cells (HPCs).Peripheral blood stem cells in circulation have become the most common source of hematopoietic stem cells intended for transplantation after minimal manipulation. Homeo box (Hox), sonic hedgehog (SHH), and Wingless-type MMTV integration site family (Wnt) are known to modulate the self-renewal and expansion of hematopoietic progenitor/stem cells in humans and mice. Unlike cytokines, Hox, SHH, and Wnt are highly conserved among species from flies to humans but studies regarding the self-renewal and expansion of the HSC are extremely limited in dogs.

REFERENCES

  1. Antonchuk, J., Sauvageau, G., Humohries, R.K. (2002). HOXB4-induced expansion of aldut hematopoietic stem cells. Exvivo. Cell 109: 39-45.
  2. Austin, T, W., Solar, G.P., Ziegler, F.C.,  Liem L., Mathews, W. (1997). A role for the Wnt gene family in hematopoiesis: expansion of multi lineage progenitor cells. Blood, 89:3624-3635.
  3. Barber, B.A. and  Rastegar, M. (2010). Epigenetic control of Hox genes during neurogenesis, development, and disease. Ann. Anat. 192: 261–274.
  4. Berg, V.B., Sharma, A.K.,  Bruno, E.,  Hoffman, R., (1998). Role of members of the Wnt gene family in human hematopoiesis. Blood, 92: 3189-3202.
  5. Borrow, J., Shearman, A.M.,  Stanton, V.P.,  Becher, R.,  Collins, T., Williams, A.J.,   Dube, I.,  Katz, F.,   Kwong, Y.L.,    Morris, C.,  Ohyashiki,  K.,  Toyama, K.  (1996). The t(7;11)(p15;p15) translocation in acute myeloid leukaemia fuses the genes for nucleoporin NUP98 and class I homeoprotein HOXA9. Nat Genet,12: 159–167.
  6. Bruno, B., Nash, R.A.,  Wallace, P.M.,  Gass, M.J.,  Thompson, J., Storb, R.,  Mc Sweeney, P.A. (1999). CD34+ selected bone marrow grafts are radioprotective and establish mixed chimerism in dogs given high dose total body irradiation. Transplantation, 68: 338-344.
  7. Daley, G.W. (2009). Hematopoietic Stem Cells.  Academic Press, pp: 211–217.
  8. Hartnett, B.J., D. Yao, S.E. Suter, N.M. Ellinwood, P.S. Henthorn, P.E. Moore, P.A.
  9. McSweeney, R.A. Nash, J.D. Brown, K.I. Weinberg and P.J. Felsburg, 2002. Transplantation of  X-linked severe combined immuno deficient dogs with CD34+ bone marrow cells. Biol. Blood Marrow Transpl. 8: 188-197.
  10. Mulgrew, N.M., Kettyle, L.M., Ramsey, J.M., Cull, S., Smyth, L.J., Mervyn, D.M., Bijl, J.J., Thompson, A. (2014). c-Met inhibition in a HOXA9/Meis1 model of CN-AML. Dev Dyn. 243:172–181.
  11. Murdoch, B., Chadwick, K., Martin, M., Shojaei, F., Shah, K., Gallacher, L., Moon, R.T.,  Bhatia, M. (2003). Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells. In vitro. Proc. Natl.Acad. Sci. U.S.A. 100: 3422-3437.
  12. Nusse, R. and H.E.Varmus. (1982). Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell,31: 99-109.
  13. Patterson, D., (2000). Companion animal medicine in the age of medical genetics. J.Vet. Intern. Med. 14: 1-9.
  14. Sato, Y., Abe, S., Mise, K., Sasaki, M., Kamada, N., Kouda, K., Musashi, M., Horikoshi, Y. Minami,Y. (1987). Reciprocal translocation involving the short arms of chromosomes 7 and 11, t(7p;11p+), associated with myeloid leukemia with maturation. Blood; 70: 1654–1658.
  15. Staal, F.J. and H.C. Clevers. (2005). Wnt signaling and hematopoiesis: a Wnt-Wnt situation. Nat.Rev.Immunol. 5: 21-30.
  16. Taghon, T., Stolz, F., De Smedt, M., Cnockaert, M., Verhasselt, B., Plum, J., Leclercq, G. (2002). HoxA10 regulated hematopoietic lineage commitment: evidence for a monocytespecific transcription factor. Blood, 99: 1197-1204.
  17. Verfallie, C.M., (2002). Hematopoietic stem cells for transplantation. Nature Immunology, vol 3, no4,pp: 314-317.
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    Research Article

    volume 39 issue 3 (september 2019) : 254-256,   Doi: 10.18805/ag.D-4929

    Real-time Expression of Stemness and Self-Renewal Genes in Canine Hematopoietic Progenitor Cells

    S
    S. Nandhini
    G
    Gowri A. Mangala
    G
    G.R. Baranidharan
    T
    T.V. Meenambigai
    1Department of Animal Biotechnology, CSCRRM, Madras Veterinary College, Chennai-7,India
    Cite article:- Nandhini S., Mangala A. Gowri, Baranidharan G.R., Meenambigai T.V. (2019). Real-time Expression of Stemness and Self-Renewal Genes in Canine Hematopoietic Progenitor Cells. Agricultural Science Digest. 39(3): 254-256. doi: 10.18805/ag.D-4929.

    ABSTRACT

    Blood cells are responsible for constant maintenance and immune protection of every cell type of the body and this relentless and brutal work requires cells that have the greatest powers of self-renewal and are designated as Hematopoietic progenitor cells (HPCs).Peripheral blood stem cells in circulation have become the most common source of hematopoietic stem cells intended for transplantation after minimal manipulation. Homeo box (Hox), sonic hedgehog (SHH), and Wingless-type MMTV integration site family (Wnt) are known to modulate the self-renewal and expansion of hematopoietic progenitor/stem cells in humans and mice. Unlike cytokines, Hox, SHH, and Wnt are highly conserved among species from flies to humans but studies regarding the self-renewal and expansion of the HSC are extremely limited in dogs.

    REFERENCES

    1. Antonchuk, J., Sauvageau, G., Humohries, R.K. (2002). HOXB4-induced expansion of aldut hematopoietic stem cells. Exvivo. Cell 109: 39-45.
    2. Austin, T, W., Solar, G.P., Ziegler, F.C.,  Liem L., Mathews, W. (1997). A role for the Wnt gene family in hematopoiesis: expansion of multi lineage progenitor cells. Blood, 89:3624-3635.
    3. Barber, B.A. and  Rastegar, M. (2010). Epigenetic control of Hox genes during neurogenesis, development, and disease. Ann. Anat. 192: 261–274.
    4. Berg, V.B., Sharma, A.K.,  Bruno, E.,  Hoffman, R., (1998). Role of members of the Wnt gene family in human hematopoiesis. Blood, 92: 3189-3202.
    5. Borrow, J., Shearman, A.M.,  Stanton, V.P.,  Becher, R.,  Collins, T., Williams, A.J.,   Dube, I.,  Katz, F.,   Kwong, Y.L.,    Morris, C.,  Ohyashiki,  K.,  Toyama, K.  (1996). The t(7;11)(p15;p15) translocation in acute myeloid leukaemia fuses the genes for nucleoporin NUP98 and class I homeoprotein HOXA9. Nat Genet,12: 159–167.
    6. Bruno, B., Nash, R.A.,  Wallace, P.M.,  Gass, M.J.,  Thompson, J., Storb, R.,  Mc Sweeney, P.A. (1999). CD34+ selected bone marrow grafts are radioprotective and establish mixed chimerism in dogs given high dose total body irradiation. Transplantation, 68: 338-344.
    7. Daley, G.W. (2009). Hematopoietic Stem Cells.  Academic Press, pp: 211–217.
    8. Hartnett, B.J., D. Yao, S.E. Suter, N.M. Ellinwood, P.S. Henthorn, P.E. Moore, P.A.
    9. McSweeney, R.A. Nash, J.D. Brown, K.I. Weinberg and P.J. Felsburg, 2002. Transplantation of  X-linked severe combined immuno deficient dogs with CD34+ bone marrow cells. Biol. Blood Marrow Transpl. 8: 188-197.
    10. Mulgrew, N.M., Kettyle, L.M., Ramsey, J.M., Cull, S., Smyth, L.J., Mervyn, D.M., Bijl, J.J., Thompson, A. (2014). c-Met inhibition in a HOXA9/Meis1 model of CN-AML. Dev Dyn. 243:172–181.
    11. Murdoch, B., Chadwick, K., Martin, M., Shojaei, F., Shah, K., Gallacher, L., Moon, R.T.,  Bhatia, M. (2003). Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells. In vitro. Proc. Natl.Acad. Sci. U.S.A. 100: 3422-3437.
    12. Nusse, R. and H.E.Varmus. (1982). Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell,31: 99-109.
    13. Patterson, D., (2000). Companion animal medicine in the age of medical genetics. J.Vet. Intern. Med. 14: 1-9.
    14. Sato, Y., Abe, S., Mise, K., Sasaki, M., Kamada, N., Kouda, K., Musashi, M., Horikoshi, Y. Minami,Y. (1987). Reciprocal translocation involving the short arms of chromosomes 7 and 11, t(7p;11p+), associated with myeloid leukemia with maturation. Blood; 70: 1654–1658.
    15. Staal, F.J. and H.C. Clevers. (2005). Wnt signaling and hematopoiesis: a Wnt-Wnt situation. Nat.Rev.Immunol. 5: 21-30.
    16. Taghon, T., Stolz, F., De Smedt, M., Cnockaert, M., Verhasselt, B., Plum, J., Leclercq, G. (2002). HoxA10 regulated hematopoietic lineage commitment: evidence for a monocytespecific transcription factor. Blood, 99: 1197-1204.
    17. Verfallie, C.M., (2002). Hematopoietic stem cells for transplantation. Nature Immunology, vol 3, no4,pp: 314-317.
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