Agricultural Science Digest

  • Chief EditorArvind kumar

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Agricultural Science Digest, volume 40 issue 2 (june 2020) : 167-170

Optimization of Protoplast Isolation and Micropropagation Techniques in Rose

Anwesha Ash, Geetanjali Chakraborty, K.V. Rashmi
1Department of Biotechnology, Sir M. Visvesvaraya Institute of Technology, Bangalore-562 157, Karnataka, India.
Cite article:- Ash Anwesha, Chakraborty Geetanjali, Rashmi K.V. (2020). Optimization of Protoplast Isolation and Micropropagation Techniques in Rose. Agricultural Science Digest. 40(2): 167-170. doi: 10.18805/ag.D-4897.
Rose is the dominant flower in the ornamental industry because of its multiple aesthetic values.  Optimization of plant growth regulators in media was achieved for one of the important species of rose. The nodal explants were tested on a full strength MS media with different hormone concentrations of 6-benzylaminopurine (BAP) and Kinetin (Kn). Maximum response was obtained for a combined hormone concentration of 6-benzylaminopurine (3.5mg/L) and kinetin (1.5mg/L) with shoot regeneration from nodes, internodes and apical meristematic tissue. 6-benzylaminopurine alone showed shoot regeneration only from internodes and nodes, while kinetin alone showed shoot regeneration only from apical meristematic tissue. Protoplast of Rosa sp. and Rosa sinensis were isolated enzymatically and fused using polyethylene. The protoplasts were observed to fuse on different planes in an irregular pattern. 
  1. Attia, A.O., Dessoky, E.D.S. and El-Tarras, A.E., (2012). In vitro propagation of Rosa hybrida L. cv. Al-Taif Rose plant. African Journal of Biotechnology. 11(48): 10888-10893.
  2. Balas, J., Coronado, P.A.G., Silva, J.A.T. and Jayatilleke, M.P., (2006). Supporting post-harvest performance of cut-    flowers using fresh-flower-refreshments and other vase-    water-additives. Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues. 1: 612-629.
  3. Boskabady M.H., Shafei M.N., Saberi Z. and Amini S., (2011). Pharmacological effects of Rosa damascena. Iranian Journal of Basic Medical Sciences. 14(4):295. 
  4. Broertjes, C. ed., (2012). Application of Mutation Breeding Methods in the Improvement of Vegetatively Propagated Crops (Vol. 2). Elsevier.
  5. Fairbrother F, (1965)” Roses”, Penguin, UK.
  6. Farahani. F. and Shaker. S., (2012). Propagation and growth from cultured single node explants of rosa (Rosa miniature). African Journal of Plant Science. 6(10): 277-281.
  7. Gudin S, (2000). Rosa: genetics and breeding. Plant Breeding Review. 17. 159-189.
  8. Hurst CC, (1941). Notes on the origin and evolution of our garden roses. Journal of the Royal Horticulture Society. 66: 73-8.2.
  9. Kajla, S., Kala, S., Kumar, A., Mir, H. and Singh, M.K.,(2018), Effect of growth regulators on in vitro shoot multiplication and plant regeneration of Rosa hybriad L. from Nodal Explants, International Journal of Current Microbiology and Applied Sciences.
  10. Khaskheli A.J., Khaskheli M.I., Khaskheli M.A., Shar T., Ahmad, W., Lighari, U.A., et al (2018). Proliferation, multiplication and improvement of micro-propagation system for mass clonal production of rose through shoot tip culture. American Journal of Plant Sciences. 9(02): 296.
  11. Krussman G, (1981). The Complete Book of Roses. Timber Press, Portland, OR
  12. Marchant, R., Davey, M.R. and Power, J.B., (1997). Isolation and culture of mesophyll protoplasts from Rosa hybrida. Plant Cell, Tissue and Organ Culture. 50(2): 131-134.
  13. Matthews, D., Mottley, J., Horan, I. and Roberts, A.V., (1991). A protoplast to plant system in roses. Plant Cell, Tissue and Organ Culture. 24(3): 173-180.
  14. Maurya, R.P., Yadav, R.C., Godara, N.R. and Beniwal, V.S., (2013). In Vitro plant regeneration of rose (Rosa hybrida L.) cv„ Benjamin Paul through various explants. Journal of Experimental Biology. 1: 2S.
  15. Mishra, N., Tandon, V.L. and Munjal, A., (2009). Evaluation of medicinal properties of Hibiscus rosasinensis in male Swiss Albino Mice. International Journal of Pharmaceutical and Clinical Research. 1(3): 106-111.
  16. Murashige, T. and Skoog, F., (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plant. 15.
  17. Nagar P.K., Sharma M., Ahuja .P.S, (2007). Rose: Some important findings with reference to physiology of flowering, Floriculture and Ornamental Biotechnology.
  18. Patel, V.R., Patel, P.R. and Kajal, S.S., (2010). Antioxidant activity of some selected medicinal plants in western region of India. Advances in Biological Research. 4(1): 23-26.
  19. Pati, P.K., Sharma, M. and Ahuja, P.S., (2008). Rose protoplast isolation and culture and heterokaryon selection by immobilization in extra thin alginate film. Protoplasma. 233(1-2): 165-171. 
  20. Saklani Kumud, Hem Pant, Vijay Rawat, (2015). Micropropagation of rose cultivars: Biotechnological application to Floriculture. Journal of Environmental Research and Development.10.
  21. Shepherd, R.E., (1954). History of the Rose. (Vol. 77, No. 5, p. 416). LWW.
  22. Šiško. M., (2011). Micropropagation of roses (Rosa spp.): the effects of different media on in vitro rooting. Agricultura (Slovenia). 8(2): 19-22. 
  23. Squirrell, J., Mandegaran, Z., Yokoya, K., Roberts, A.V. and Mottley, J., (2005). Cell lines and plants obtained after protoplast fusions of Rosa+Rosa, Rosa+Prunus and Rosa+Rubus. Euphytica. 146(3): 223-231.
  24. Xia, Y., Deng, X., Zhou, P., Shima, K. and Teixeira da Silva, J.A., (2006). The World floriculture industry: dynamics of production and markets. Floriculture, Ornamental and Plant Biotechnology, Adv. Trop Issues. 4: 336-347.
  25. Younis, A., Khan, M.A., Khan, A.A., Riaz, A. and Pervez, M.A., (2007). Effect of different extraction methods on yield and quality of essential oil from four Rosa species. Floriculture and Ornamental Biotechnology. 1(1): 73-76.

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