Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 38 issue 4 (december 2018) : 265-269,   Doi: 10.18805/ag.D-4821

Molecular characterization of Asplenium nidus L. – A potential ornamental fern species from Andaman

K
K. Abirami
V
V. Baskaran
P
P. Simhachalam
K
K. Venkatesan
D
D.R. Singh
1ICAR-Central Island Agricultural Research Institute, Port Blair-744 101, Andaman and Nicobar Islands, India.
Cite article:- Abirami K., Baskaran V., Simhachalam P., Venkatesan K., Singh D.R. (2019). Molecular characterization of Asplenium nidus L. – A potential ornamental fern species from Andaman. Agricultural Science Digest. 38(4): 265-269. doi: 10.18805/ag.D-4821.

ABSTRACT

Asplenium nidus L. is one of the most important terrestrial fern in Andaman and Nicobar Islands with great ornamental value. Inter simple sequence repeat (ISSR) analysis was used to study the genetic diversity of the species A. nidus L. collected from different geographical locations of the Island. Among 15 ISSR primers screened, 9 primers produced amplification and produced a total of 222 reproducible amplified bands. The number of amplified bands varied from 10 in ISSR 11 to 44 in UBC 842 with an average of 24.7 bands per primer. The range of polymorphic bands and percent polymorphism observed were 1 to 5 and 33.3 to 83.3, respectively. The polymorphic information content value of ISSR marker ranged from 0.365 to 0.485. Cluster analysis grouped the twelve accessions into three clusters (Cluster I, II and III) at Jaccard’s similarity coefficient value of 0.69 on basis of geographical locations of the accessions. The cluster I was further divided into two sub-clusters Ia and Ib at the coefficient value of 0.77 and it includes the accessions belonging to South Andaman, one each accession collected from two different locations, Little Andaman and Rangat (Middle Andaman) were grouped separately as clusters II and III, respectively. The genetic variability studies with ISSR marker grouped the accessions of A. nidus L.on basis of their geographical locations and it would help in rapid identification of polymorphism and assist in germplasm collection, conservation and domestication programmes. 

REFERENCES

  1. Abirami, K., Singh, D R., Simhachalam, P. and Baskaran, V. (2016). Genetic diversity analysis of medicinal plant Casssia alata in Andaman Island through ISSR markers. Indian J. Experimental Agri. Inter., 14(4): 1-6.
  2. Abirami, K., Baskaran,V. and Simhachalam, P. (2018). ISSR marker based diversity assessment of Piper spp. in Bay Islands, India.Indian J. Agrl. Res.,52(4): 434-438.
  3. Ahmad, I., Bhagat, S., Sharma, T. V. R. S., Kumar, K. and Simachalam, P. (2010). ISSR and RAPD marker based DNA fingerprinting and diversity assessment of Annona spp. in South Andaman. Indian J. Hort.,67(2): 147-151. 
  4. Aitchitt, M., Ainsworth, C. C. and Thangavelu, M.(1993). A rapid and efficient method for the extraction of total DNA from mature leaves of the date palm (Phoenix dactylifera L.). Plant Mol. Biol. Rep., 11(4):317-319.
  5. Asha, K. L., Nair, G. M. and Nair, M. C. (2006). Inter-relationship among the species of Dioscorea revealed by morphological trait and RAPD marker. Indian J. Plant Genet. Resources.19: 40-46. 
  6. Burkhill, I. H.(1935).A dictionary of the Economic Products of Malay Peninsular. Ministry of Agriculture, Malaysia, 1542.
  7. Ghafoor, S., Shah, M. M. and Ahmad, H. (2007). Molecular characterization of Ephedra species found in Pakistan. Genet. Mol. Res. 6: 1123-1130.
  8. Gupta, M., Chyi, Y. S., Romero-Severson. and Jowen, J. L. (1994). Amplification of DNA markers from evolutionary diverse genomes using simple sequence repeats. Theor.Appl. Genet.,89:998-1006.
  9. Joshi, S. P., Gupta, V.S., Aggarwal, R.K. and Ranjekar, P. K. (2000). Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor. Appl. Genet.,100: 1311-1320. 
  10. Nath, K., Bhattacharya,M. K., Sen, A. and Kar, S. (2013) Antibacterial activity of frond extract of Asplenium nidus L., a threatened ethnomedicinal fern of North East India. International J. Pharmacog. and Phytochem., 28(2): 1169-1172.
  11. Powell, W., Margenta, M., Andrew C., Hanfrey, M., Vogel, J., Tingey, S. and Rafalsky, A. (1996). The utility of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breed.,2: 225-238.
  12. Rohlf, F.J. (1998). NTSYS-PC Numerical Taxonomyand Multivariate Analysis System Version 2.02,Exeter Publications Setauket, New York.
  13. Sneath, P.H.A. and Sokal, R.R. (1973). NumericalTaxonomy - The Principles and Practice of Numerical Classification. Freeman and Co. SanFrancisco. P. 573.
  14. Vinod, K, Singh, G., Sharma, R. and Sharma, S. N. (2007) RAPD and protein profiles of cotton varieties. Indian J. Plant Physiol.,12: 115-119.
  15. Yatabe Y, S., Masuyama, D., Darnaedi. and Murakami, N. (2001). Molecular systematic of the Asplenium nidus complex from Mt. Halimun National park, Indonesia: Evidence for reproductive isolation among three sympatric RBCL sequence types. American J. Bot., 88(8): 1517-1522.
  16. Zietkiewicz E., Rafalski, A. and Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification. Genomics,20: 176-183. 
Published In
Agricultural Science Digest
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 38 issue 4 (december 2018) : 265-269,   Doi: 10.18805/ag.D-4821

    Molecular characterization of Asplenium nidus L. – A potential ornamental fern species from Andaman

    K
    K. Abirami
    V
    V. Baskaran
    P
    P. Simhachalam
    K
    K. Venkatesan
    D
    D.R. Singh
    1ICAR-Central Island Agricultural Research Institute, Port Blair-744 101, Andaman and Nicobar Islands, India.
    Cite article:- Abirami K., Baskaran V., Simhachalam P., Venkatesan K., Singh D.R. (2019). Molecular characterization of Asplenium nidus L. – A potential ornamental fern species from Andaman. Agricultural Science Digest. 38(4): 265-269. doi: 10.18805/ag.D-4821.

    ABSTRACT

    Asplenium nidus L. is one of the most important terrestrial fern in Andaman and Nicobar Islands with great ornamental value. Inter simple sequence repeat (ISSR) analysis was used to study the genetic diversity of the species A. nidus L. collected from different geographical locations of the Island. Among 15 ISSR primers screened, 9 primers produced amplification and produced a total of 222 reproducible amplified bands. The number of amplified bands varied from 10 in ISSR 11 to 44 in UBC 842 with an average of 24.7 bands per primer. The range of polymorphic bands and percent polymorphism observed were 1 to 5 and 33.3 to 83.3, respectively. The polymorphic information content value of ISSR marker ranged from 0.365 to 0.485. Cluster analysis grouped the twelve accessions into three clusters (Cluster I, II and III) at Jaccard’s similarity coefficient value of 0.69 on basis of geographical locations of the accessions. The cluster I was further divided into two sub-clusters Ia and Ib at the coefficient value of 0.77 and it includes the accessions belonging to South Andaman, one each accession collected from two different locations, Little Andaman and Rangat (Middle Andaman) were grouped separately as clusters II and III, respectively. The genetic variability studies with ISSR marker grouped the accessions of A. nidus L.on basis of their geographical locations and it would help in rapid identification of polymorphism and assist in germplasm collection, conservation and domestication programmes. 

    REFERENCES

    1. Abirami, K., Singh, D R., Simhachalam, P. and Baskaran, V. (2016). Genetic diversity analysis of medicinal plant Casssia alata in Andaman Island through ISSR markers. Indian J. Experimental Agri. Inter., 14(4): 1-6.
    2. Abirami, K., Baskaran,V. and Simhachalam, P. (2018). ISSR marker based diversity assessment of Piper spp. in Bay Islands, India.Indian J. Agrl. Res.,52(4): 434-438.
    3. Ahmad, I., Bhagat, S., Sharma, T. V. R. S., Kumar, K. and Simachalam, P. (2010). ISSR and RAPD marker based DNA fingerprinting and diversity assessment of Annona spp. in South Andaman. Indian J. Hort.,67(2): 147-151. 
    4. Aitchitt, M., Ainsworth, C. C. and Thangavelu, M.(1993). A rapid and efficient method for the extraction of total DNA from mature leaves of the date palm (Phoenix dactylifera L.). Plant Mol. Biol. Rep., 11(4):317-319.
    5. Asha, K. L., Nair, G. M. and Nair, M. C. (2006). Inter-relationship among the species of Dioscorea revealed by morphological trait and RAPD marker. Indian J. Plant Genet. Resources.19: 40-46. 
    6. Burkhill, I. H.(1935).A dictionary of the Economic Products of Malay Peninsular. Ministry of Agriculture, Malaysia, 1542.
    7. Ghafoor, S., Shah, M. M. and Ahmad, H. (2007). Molecular characterization of Ephedra species found in Pakistan. Genet. Mol. Res. 6: 1123-1130.
    8. Gupta, M., Chyi, Y. S., Romero-Severson. and Jowen, J. L. (1994). Amplification of DNA markers from evolutionary diverse genomes using simple sequence repeats. Theor.Appl. Genet.,89:998-1006.
    9. Joshi, S. P., Gupta, V.S., Aggarwal, R.K. and Ranjekar, P. K. (2000). Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor. Appl. Genet.,100: 1311-1320. 
    10. Nath, K., Bhattacharya,M. K., Sen, A. and Kar, S. (2013) Antibacterial activity of frond extract of Asplenium nidus L., a threatened ethnomedicinal fern of North East India. International J. Pharmacog. and Phytochem., 28(2): 1169-1172.
    11. Powell, W., Margenta, M., Andrew C., Hanfrey, M., Vogel, J., Tingey, S. and Rafalsky, A. (1996). The utility of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breed.,2: 225-238.
    12. Rohlf, F.J. (1998). NTSYS-PC Numerical Taxonomyand Multivariate Analysis System Version 2.02,Exeter Publications Setauket, New York.
    13. Sneath, P.H.A. and Sokal, R.R. (1973). NumericalTaxonomy - The Principles and Practice of Numerical Classification. Freeman and Co. SanFrancisco. P. 573.
    14. Vinod, K, Singh, G., Sharma, R. and Sharma, S. N. (2007) RAPD and protein profiles of cotton varieties. Indian J. Plant Physiol.,12: 115-119.
    15. Yatabe Y, S., Masuyama, D., Darnaedi. and Murakami, N. (2001). Molecular systematic of the Asplenium nidus complex from Mt. Halimun National park, Indonesia: Evidence for reproductive isolation among three sympatric RBCL sequence types. American J. Bot., 88(8): 1517-1522.
    16. Zietkiewicz E., Rafalski, A. and Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification. Genomics,20: 176-183. 
    In this Article
      Contact us
      Follow us
      Published In
      Agricultural Science Digest

      Editorial Board

      View all (0)