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Comparison of Methods for Genomic Deoxyribonucleic Acid (DNA) Extraction Suitable for Whole-Genome Genotyping in Traditional Varieties of Rice

DOI: 10.18805/ag.D-4768    | Article Id: D-4768 | Page : 228-231
Citation :- Comparison of Methods for Genomic Deoxyribonucleic Acid (DNA) Extraction Suitable for Whole-Genome Genotyping in Traditional Varieties of Rice.Agricultural Science Digest.2019.(39):228-231
B. Priyadharshini, M. Vignesh, M. Prakash and R. Anandan
geeth_prakash@yahoo.co.in
Address :
Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai nagar, Tamilnadu, India.
Submitted Date : 2-06-2018
Accepted Date : 17-06-2019

Abstract

The utilization and conservation traditional rice genotypes have attracted global attention. Optimization of DNA isolation protocol for genetic characterization of plants is a necessary and primary step. Over the recent years, next generation sequencing and microarray technologies have revolutionized scientific research, with their applications to high-throughput analysis of biological systems. Isolation of high quantities of pure, intact, double stranded, highly concentrated DNA is prerequisite for successful and reliable large-scale genotyping analysis. Therefore, standardization of DNA isolation is a basic requirement. Here we employed three methods of DNA isolation namely, Dellaporta, Hi-purA and modified CTAB techniques for isolation of genomic DNA from 25 indigenous rice genotypes. From the results, it was found that genomic DNA isolated by modified CTAB method to be the most appropriate for extracting high quality and maximum quantity DNA suitable for genotyping.  The quality and quantity of the differentially extracted DNA was subsequently assessed by spectrophotometric measurements and gel electrophoresis.

Keywords

Isolation CTAB UV nano-spectrophotometer gel electrophoresis UV gel documentation

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