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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 37 issue 3 (september 2017) : 247-248,   Doi: 10.18805/asd.v37i03.8989

In vitro evaluation of bio-agents against Pyricularia oryzae (Cav.) causing rice blast disease

A
Akhilesh Kumar Kulmitra
N
Neha Sahu
V
V.B. Sanath Kumar
T
Thejesha A.G.
A
Amlan Ghosh
Y
Yasmin Gulnaz
1Department of Plant Pathology, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bangalore-560065, Karnataka, India.
Cite article:- Kulmitra Kumar Akhilesh, Sahu Neha, Kumar Sanath V.B., A.G. Thejesha, Ghosh Amlan, Gulnaz Yasmin (2017). In vitro evaluation of bio-agents against Pyricularia oryzae (Cav.) causing rice blast disease. Agricultural Science Digest. 37(3): 247-248. doi: 10.18805/asd.v37i03.8989.

ABSTRACT

The five different bio-agents viz., Trichoderma viride, T. harzianum, T. virens, Pseudomonas fluorescens and Bacillus subtilis were evaluated against Pyricularia oryzae at four and eight days after incubation through dual culture technique. Among the five different bio-agents, highest per cent inhibition of mycelial growth of fungus was recorded in T. virens i.e. 67 per cent and 70 percent after four and eight days after incubation respectively with mean of 68.5 per cent followed by Trichoderma viride with the inhibition of 61 and 63 per cent respectively with mean of 62 per cent. The Pseudomonas fluorescens did not show any inhibition of mycelial growth of P. oryzae as the pathogen over grew the bio-agents. 

REFERENCES

  1. Ali, H. and Nadarajah, K., (2014). Evaluating the efficacy of Trichoderma sp. and Bacillus substilis as biocontrol agents against Magnaporthe grisea in rice. Aus. J. crop. Sci., 8(9): 1324-1335.
  2. Anonymous, (2016). Statistical database. www.fao.org.
  3. Arumugam, K., Ramalingam, P. and Appu, M., (2013). Isolation of Trichoderma viride and Pseudomonas fluorescens organism from soil and their treatment against rice pathogens. J. Microbiol. Biotech. Res., 3(6): 77-81.
  4. Ghose RL, Ghatage MB and Subrahmanyan, V., (1960). Disease, Rice in India., pp. 67.
  5. Gnanamanickam, S. S. and Mew, T. W., (1992). Biological control of blast disease of rice (Oryza sativa L.) with antagonistic bacteria and its mediation by a Pseudomonas antibiotic. Ann. Phytopathol. Soc. Japan. 58: 380-385.
  6. Gomez, K. A. and Gomez, A. A., (1984). Statistical Procedures for Agricultural Research with emphasis on rice. International Rice Research Institute, Los Banos, Philippines, pp. 268.
  7. Gouramanis, G. D., (1997). Biological and chemical control of rice blast disease in North Greece. Cahiers Options Mediterraneennes, 15: 61-68.
  8. Hajano, J., Lodhi, M. A., Mumtaz, A. P., Khanzada, M. A. and Shah, S. G., (2012). In vitro evaluation of fungicides, plant extracts and bio-control agents against rice blast pathogen Magnaporthe oryzae Couch. Pak. J. Bot., 44(5): 1775-1778.
  9. Hosmand, R. A., (1988). Statistical Methods for Agricultural Sciences. Timber Press, Portland, Oregon, USA., pp. 405.
  10. Joshi, R. and Gardener., B. M., (2006). Identification and characterization of novel genetic markers associated with biological control activities in Bacillus subtilis. Phytopathology, 96(2): 145-154.
  11. Karthikeyan, V. and Gnanamanickam, S. S., (2008). Biologocal control of Setaria blast (M. Grisea) with bacterial strains, Crop Protection, 27(2): 263-267.
  12. Vincent, J. M., (1947). Distribution of fungal hyphae in the presence of certain inhibitors. Nature, 159: 850.
  13. Watanabe, N., (1985). Antagonism by various kinds of Trichoderma fungi to airborne plant pathogens. Bulletin of the Faculty of Agriculture Meiji University, 68: 1-9.
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    Research Article

    volume 37 issue 3 (september 2017) : 247-248,   Doi: 10.18805/asd.v37i03.8989

    In vitro evaluation of bio-agents against Pyricularia oryzae (Cav.) causing rice blast disease

    A
    Akhilesh Kumar Kulmitra
    N
    Neha Sahu
    V
    V.B. Sanath Kumar
    T
    Thejesha A.G.
    A
    Amlan Ghosh
    Y
    Yasmin Gulnaz
    1Department of Plant Pathology, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bangalore-560065, Karnataka, India.
    Cite article:- Kulmitra Kumar Akhilesh, Sahu Neha, Kumar Sanath V.B., A.G. Thejesha, Ghosh Amlan, Gulnaz Yasmin (2017). In vitro evaluation of bio-agents against Pyricularia oryzae (Cav.) causing rice blast disease. Agricultural Science Digest. 37(3): 247-248. doi: 10.18805/asd.v37i03.8989.

    ABSTRACT

    The five different bio-agents viz., Trichoderma viride, T. harzianum, T. virens, Pseudomonas fluorescens and Bacillus subtilis were evaluated against Pyricularia oryzae at four and eight days after incubation through dual culture technique. Among the five different bio-agents, highest per cent inhibition of mycelial growth of fungus was recorded in T. virens i.e. 67 per cent and 70 percent after four and eight days after incubation respectively with mean of 68.5 per cent followed by Trichoderma viride with the inhibition of 61 and 63 per cent respectively with mean of 62 per cent. The Pseudomonas fluorescens did not show any inhibition of mycelial growth of P. oryzae as the pathogen over grew the bio-agents. 

    REFERENCES

    1. Ali, H. and Nadarajah, K., (2014). Evaluating the efficacy of Trichoderma sp. and Bacillus substilis as biocontrol agents against Magnaporthe grisea in rice. Aus. J. crop. Sci., 8(9): 1324-1335.
    2. Anonymous, (2016). Statistical database. www.fao.org.
    3. Arumugam, K., Ramalingam, P. and Appu, M., (2013). Isolation of Trichoderma viride and Pseudomonas fluorescens organism from soil and their treatment against rice pathogens. J. Microbiol. Biotech. Res., 3(6): 77-81.
    4. Ghose RL, Ghatage MB and Subrahmanyan, V., (1960). Disease, Rice in India., pp. 67.
    5. Gnanamanickam, S. S. and Mew, T. W., (1992). Biological control of blast disease of rice (Oryza sativa L.) with antagonistic bacteria and its mediation by a Pseudomonas antibiotic. Ann. Phytopathol. Soc. Japan. 58: 380-385.
    6. Gomez, K. A. and Gomez, A. A., (1984). Statistical Procedures for Agricultural Research with emphasis on rice. International Rice Research Institute, Los Banos, Philippines, pp. 268.
    7. Gouramanis, G. D., (1997). Biological and chemical control of rice blast disease in North Greece. Cahiers Options Mediterraneennes, 15: 61-68.
    8. Hajano, J., Lodhi, M. A., Mumtaz, A. P., Khanzada, M. A. and Shah, S. G., (2012). In vitro evaluation of fungicides, plant extracts and bio-control agents against rice blast pathogen Magnaporthe oryzae Couch. Pak. J. Bot., 44(5): 1775-1778.
    9. Hosmand, R. A., (1988). Statistical Methods for Agricultural Sciences. Timber Press, Portland, Oregon, USA., pp. 405.
    10. Joshi, R. and Gardener., B. M., (2006). Identification and characterization of novel genetic markers associated with biological control activities in Bacillus subtilis. Phytopathology, 96(2): 145-154.
    11. Karthikeyan, V. and Gnanamanickam, S. S., (2008). Biologocal control of Setaria blast (M. Grisea) with bacterial strains, Crop Protection, 27(2): 263-267.
    12. Vincent, J. M., (1947). Distribution of fungal hyphae in the presence of certain inhibitors. Nature, 159: 850.
    13. Watanabe, N., (1985). Antagonism by various kinds of Trichoderma fungi to airborne plant pathogens. Bulletin of the Faculty of Agriculture Meiji University, 68: 1-9.
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