Biochemical study of root extract of Withania somnifera (L.) plant through HPLC analysis 

DOI: 10.18805/asd.v36i4.6471    | Article Id: D-4381 | Page : 295-298
Citation :- Biochemical study of root extract of Withania somnifera (L.)plant through HPLC analysis .Agricultural Science Digest.2016.(36):295-298

Naveen Gaurav1, Arun Kumar1, Aditi Grover1, Deepak Som2, U.K. Chauhan3 and A. P. Singh4

Address :

Department of Biotechnology, S G R R P G College, Dehradun- 248 001, U.K.

Submitted Date : 22-02-2016
Accepted Date : 5-10-2016


Secondary metabolite contents of W. somnifera varied remarkably between seasons and genotypes under ex vitro condition. In vitro studies provide an optimum culture condition for steady and quality production of bioactive chemicals throughout the year without involvement of environmental stresses. Mass production of micro-shoots and plantlets by exploring the organogenic totipotency of shoot tip explants (ex vitro and in vitro grown) considering two elite genotypes (Poshita and Jawahar 22) of W. somnifera, and assessment of their capability in production and accumulation of bioactive metabolites (total alkaloid and withanolides amount were quantified; withanolide A and withaferin A contents were estimated by High Performance Liquid Chromatography-HPLC).


Bioactive chemicals Culture condition Ex vitro In vitro Mass production Micro-shoots Secondary metabolite W. somnifera.


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