Efficacy of bioagents and botanicals on seed mycoflora and seed quality in mungbean [Vigna radiata (L.) Wiczek]

DOI: 10.18805/asd.v35i1.9306    | Article Id: D-4185 | Page : 30-34
Citation :- Efficacy of bioagents and botanicals on seed mycoflora and seed qualityin mungbean [Vigna radiata (L.) Wiczek] .Agricultural Science Digest.2016.(36):30-34

S.B. Gawade, S.R. Zanjare, A.V. Suryawanshi  and V.R. Shelar*  

Address :

Seed Technology Research Unit (NSP), M.P.K.V., Rahuri- 413 722, (M.S) India.

Submitted Date : 17-11-2014
Accepted Date : 5-01-2016


Seeds of mungbean  (Cv. Vaibhav) was treated with bioagents and botanicals  viz; talc powder formulations of Pseudomonas fluorescens (0.6%), Trichoderma viride  (0.6%), Pseudomonas fluorescens + Trichoderma viride (0.6%) each, garlic extract (1%), ginger extract (1%) and Thiram + Carbendazim (0.2% each) as a recommended fungicidal check. The results indicated that, among the bioagents the treatment of  Pseudomonas fluorescens + Trichoderma viride (0.6% each) to the seeds of mungbean  was effective in reducing seed mycoflora  viz; Fusarium oxysporum, Alternaria alternata, Macrophomina phaseolena, Aspergillus flavus, Aspergillus niger and Fusarium moniliforme  by 88.5, 85.1, 64.9, 81.1, 75.0 and 100  per cent, respectively over untreated control. The seed germination, seedling vigour index and field emergence of mungbean  was increased by 21.81, 34.03 and 15.98 per cent, respectively with this treatment over untreated control. The seed germination, seedling vigour index and field emergence in untreated control was 71.83 per cent, 2261.52 and 67.83 per cent, respectively.   


Botanicals Pseudomonas fluorescens Seed mycoflora Trichoderma viride.


  1. Abdul Baki, A. A. and Anderson J. P. (1973). Vigour determination in soybean seed by multiple criteria. Crop Science., 13 : 630-633.
  2. Abdul Latif M., Abu Kaoser M. Sateh, M. Ashik Iqbal Khan, Habibur Rahman and Anwar Hussain M. (2006). Efficacy of some plant extract in controlling seed borne fungal infections of mustard. Bangladesh J. Microbial., 23 : 168-170. 
  3. Anonymous (1999). International rules for seed testing. Seed Sci. and Technol., 4 : 1-180.
  4. Bambode, R. S. and Sukla V. N. (1973). Antifungal properties of certain plants extracts against some fungi. PKV., Res. J., 2: 1-8.
  5. Bansal, R. K. and Sobti A. K. (1990). An economic remedy for the control of two species of Aspergillus on groundnut. Indian Phytopath., 43 : 451-452. 
  6. Barnett, H. L. and Barry B. H. (1972). Illustrated Genera of Imperfect fungi, Burgess Publ. Comp., Minneapolus, Minnesota.
  7. Damale, P. G. and Raut S. A. (2011). Efficacy of chemicals and bioagents on Mungbean seed germination, vigour index and association of fungi in storage. National Seed Congress on “ Quality Seed for Prosperity “ held at Pune during Jan. 29-31 : pp 252. 
  8. Fakir, G. A. (1983). Pulse disease and its control. Rept. Plant Path. Bangaladesh Agric. Univ. Mymensingh pp 17.
  9. Gohil V. P. and Valla D. G. (1996). Effect of extract of some medicinal plants on growth of Fusarium moniliforme. Indian J. Mycol. Pl. Pathol., 26 : 110.
  10. Gurjar, K. L., Singh S. D and Rawal P. (2004). Management of seed borne pathogens of okra with bio-agents. Pl. Dis. Res., 19: 44-46.
  11. Kar, A. K. and Sahu K. C. (2008). Effect of some biological agents on Macrophomina phaseolina causing seed rot, seedling blight and leaf blight of mungbean National symposium on Plant disease scenario in organic Agriculture for eco-friendly sustainability. Jan. 10-12 : 59.
  12. Khare, M. N. and Chaubey R. (1978). Significance, methods of detection and control of five species of Fusarium associated with mung seeds. Nat. Acad. Sci. India. 1.
  13. Kulshreshtha, D. O. (1988). Seed borne infection of Fusariella hughesii in mung. Curr. Sci., 37: 384-386. 
  14. Manoranjithan, S. K. and Prakasan V. (1999). Biological control of damping off disease of tomato. Sout India Hort., 47: 302-303.
  15. Neergaard, P. (1977). Seed Pathology. Vol. I. published by Halsted Press Book, Div. of John Viley and Sons, New York.
  16. Patil S. B., Memane S. A. and Konde S. K. (1990). Occurrence of seed borne fungi of green gram. J. Maharashtra Agri. Univ., 15:44-45.
  17. Prajapati, R. K., Kartha, K. K. Joshi R. K. and Nema K. G. (2003). A. Rhizoctonia disease of mung (Phaseolus aureus) Proxb. JNKVV Res. J., 3 : 40-43.
  18. Raut, J. G. and Ahire S. P. (1988). Seed borne fungi of green gram in Vidharbha and their control. PKV. Res. J., 12: 136-138.
  19. Saxena, R. M. (1986). Antagonism among seed mycoflora associated green gram and black gram. Indian J. Pl. Path., 4: 193-194.
  20. Singh, O. P. (1992). Fungicidal seed treatment for control of seed borne fungi and improving germination percentage in different soybean cultivars. Seed Tech. News : 22: 35-36.
  21. Upadhyay, J. P., Lal, H. C. and Ojha K. L. (2000). Biological control of seed borne pathogens in pulse crops. Proc. National Symp. on “Management of Biotic and Abiotic Stresses in Pulse Crops” held at Kanpur during 26-28th June : 21-26. 

Global Footprints