Production of indole-3-acetic acid by berseem (Trifolium alexandrinum L.) rhizobia isolated from Haryana, India

DOI: 10.5958/0976-0547.2015.00053.1    | Article Id: D-4177 | Page : 229-232
Citation :- Production of indole-3-acetic acid by berseem (Trifolium alexandrinum L.) rhizobia isolated from Haryana, India.Agricultural Science Digest.2015.(35):229-232
Varsha Garg, Kamlesh Kukreja, Rajesh Gera and Ankit Singla varshagarg9@gmail.com
Address : Department of Microbiology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, Hisar-125 004, Haryana, India.


A total of 40 Rhizobium strains were isolated from the root nodules of Berseem (Trifolium alexandrinum L.) crop which was collected from 17 different villages of three districts (Hisar, Sirsa and Karnal) of Haryana state. Morphologically, biochemically and finally by plant infectivity test, they were recognized as rhizobia. All the isolated strains were tested for the production of indole-3-acetic acid (a phytohormone having important physiological implications). IAA production was checked quantitatively in YEM (yeast extract mannitol) medium supplemented with 0.1 mgml-1 L-tryptophan. All the 40 Rhizobium strains were found to be IAA producers after 72 h; however their production amount varied significantly. Rhizobium strain BH14 was found to elaborate maximum amount of IAA (176 µgml-1) followed by BH13 (147.6 µgml-1) and BH45 (147.4 µgml-1). All the three superior IAA producers belonged to district Hisar. These results confirm the potential of some strains of R. leguminosarum bv trifolii as biofertilizers.


Berseem (Trifolium alexandrinum L.) Indole-3-acetic acid (IAA) Phytohormone Rhizobium.


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