Agricultural Science Digest

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Agricultural Science Digest, volume 43 issue 1 (february 2023) : 81-86

Arbuscular Mycorrhizal Fungi Symbiosis in Durum Wheat (Triticum durum Desf.) under No-tillage and Tillage Practices in a Semiarid Region

Mahieddine Sebbane1,*, Sonia Labidi2, Miloud Hafsi1
1Laboratory for the Improvement and Development of Plant and Animal Production (LADPVA), Ferhat Abbas Setif University, Setif 19000, Algeria.
2Laboratory of Horticultural Sciences, National Agronomic Institute of Tunisia, University of Carthage, LR13AGR01, 1082 Tunis, Mahrajène, Tunisia.
Cite article:- Sebbane Mahieddine, Labidi Sonia, Hafsi Miloud (2023). Arbuscular Mycorrhizal Fungi Symbiosis in Durum Wheat (Triticum durum Desf.) under No-tillage and Tillage Practices in a Semiarid Region . Agricultural Science Digest. 43(1): 81-86. doi: 10.18805/ag.D-339.
Background: Arbuscular mycorrhizal fungi (AMF) are root symbionts that improve host plant growth and resilience against biotic and abiotic stresses allowing a sustain plant production particularly under harsh conditions.

Methods: The objective of this study is to compare the effect of conventional tillage and no-tillage practices on AMF symbiosis with durum wheat (Triticum durum Desf) through the evaluation of root colonization and AMF spore density in the soil of three different sites in a semi-arid region in Algeria. Two sites were conducted under rain-fed conditions and one was irrigated.

Result: Mycorrhizal root colonization varied according to the site and the tillage practice, while spore density differed between sites. Spore density was higher in sites under rain-fed conditions suggesting that water deficit stimulate sporulation, while root colonization seems to be limited by drought. No-tillage improved root colonization rates by 54.3% compared to conventional tillage system and this improvement, particularly the arbuscular percentage, was higher under drought conditions. In addition, root colonization rates showed a positive correlation with the organic matter content and pH in the soil and a negative correlation with the available phosphorus in the soil. These results indicate that no-tillage enhance the establishment of AMF symbiosis with durum wheat under semi-arid conditions.
Durum wheat (Triticum durum Desf.) presents the first field crop grown in Algeria. Mostly cultivated under rain-fed conditions, its production is often restricted by rainfall scarcity and irregularity that characterizes the Mediterranean climate. Arbuscular mycorrhizal fungi (AMF), thanks to their benefits, can help to overcome these constraints. AMF are root symbionts that promote the absorption of water and nutrients of the host plant in exchange of carbonic substances, which can affect positively plant performance (Smith and Smith, 2011). AMF have been recognized for improving plant growth and resilience against biotic and abiotic stresses, as they also improve soil health and structure (Thirkell et al., 2017), which gives them a promising role in improving and sustaining crop production, especially under stress conditions.

Conventionally, durum wheat cultivation is carried out on plowed fields because tillage improves soil structure, minimizes weed infestation, homogenizes soils and fertilizers, and thus improves productivity. However, tillage could affect the stability and the fertility of soil, which threatens the sustainability of production. For this reason, no-tillage has been suggested as part of conservation agriculture to sustain crop production (Chen et al., 2009). Promoted by the ecological services provided, the adoption of no-till is extending worldwide (Marandola et al., 2019). While in Algeria, its adoption is still low, but an increase of technical support and the awareness of farmers can increase its extension (Rouabhi et al., 2018a).

Besides AMF and host plant traits, environmental factors including agricultural practices such as tillage system, can influence AMF symbiosis (Yang et al., 2014) and thus, the benefits that could be provided by these fungi. Therefore, information on the effect of different agricultural practices on AMF in different contexts is essential to optimize their role in sustainable production of crops. In this study, AMF symbiosis in durum wheat conducted with conventional tillage and with no-tillage was assessed through the evaluation of root colonization and spore density in three sites located in a semi-arid region.
Study area and sampling

The study was carried out in Setif (Algeria) on three sites: Remada, Station and Benifouda (Table 1) during the season 2016/2017. At each site, two durum wheat plots (10 m × 50 m) were selected, one next to another: one under traditional tillage system (T) consisting of deep plough (40 cm) followed by secondary tillage with disk harrow (15 cm). The second plot was conducted under no-tillage (NT) practice or direct seeding. The region of the study is characterized by a semi-arid climate with fresh winter and an average annual rainfall of 400 mm (Rouabhi et al., 2018b). At the heading stage, three samples of roots and rhizospheric soil (0-20 cm) were collected from each field. The samples were taken 15 m apart along the diagonal.

Table 1: Location, altitude, wheat cultivar and the cultivation system (Rainfed/ Irrigated) of the studied sites.


The soil texture was determined with the wet sieving method using the USDA texture triangle. The pH and the electrical conductivity (EC) were measured on a soil suspension (soil/water: 1/5) with a pH meter and conduct-meter. Available phosphorus (P) was determined according to the Olsen extraction method (Olsen et al., 1954). The organic carbon as the content of organic matter (OM) were measured  according to the method of Anne (1945) and the total nitrogen (N) was determined according to Kjedahl Method (Bremner et al. 1982).

To evaluate root colonization, cleaned roots were soaked in a solution of KOH (10%) then stained with a solution of trypan blue (0.05%) according to the method described by Phillips and Hayman (1970). Then, following the method of McGonigle et al., (1990), total root colonization rate as well as arbuscular and vesicular colonization rates were measured with microscopic observation (x400) of 60 root fragments of 1 cm length from each sample.

AMF spores were extracted from the soil following the wet sieving method (Gerdemann and Nicolson, 1963) and counted under a binocular loupe (x40).

Data analysis

Using XLStat 2019, the significance effect of Tillage system, Site and their interaction (Tillage system*Site) on measured parameters was tested by a two-way variance analysis, the comparison between the means of different treatments was done using LSD test and the correlations between AMF root colonization rates and soil characteristics were calculated.
Field climatic conditions and soil characteristics

The agricultural campaign (2016/2017) was characterized by a severe and long period of drought during the spring season: March, April, May (Fig 1). This drought coincided with the full growth phase of the studied species: durum wheat, when the need for water is high and could therefore affect the nutritional status of the plant and by consequence the association with AMF.

Fig 1: Ombrothermic diagram of the agricultural season 2016/2017 in Setif (Algeria).

Except for the no-tilled (NT) plot at the Station site, which had a silty texture, the soil in all of the studied plots was clay-silty. EC ranged from 2.23 (ds/m) to 3.42 (ds/m), indicating a slight salinity of the studied soils. The pH ranged from 7.14 to 7.43 indicating a neutral soil in Benifouda and Station and slightly alkaline soil in Remada (Horneck et al., 2011). The OM content, which ranged between 2.1 and 3.14%, was slightly higher in the NT plots compared to the tilled ones, especially at Remada and Station sites.

The available P amounts (ppm) were low in Remada (8.48) and average in Benifouda (15.74) for both treatments, whereas at the Station site, they were average in the no-tilled plot (15.56) and high in the tilled plot (27.75) (Horneck et al., 2011). Concerning total N content, the levels recorded in Benifouda (0.23%) were higher than those in Station (0.15%) and Remada (0.16%) sites (Table 2).

Table 2: Physico-chemical characteristics of the studied soils in the different fields.

AMF root colonization and Spore density

Even if it is not certain that AMF root colonization (RC) is translated into a better performance of the host plant (Thirkell et al., 2017), high RC rates have often been correlated with increased yields of wheat and other field crops (Zhang et al., 2019). This suggests that RC could be used to estimate the potential benefits from this symbiosis.

The analysis of variance results (Table 3) showed a significant effect of the site and the tillage system on total colonization rate (TCR), arbuscular colonization rate (ACR) and vesicular colonization rate (VCR), while the combined effect (Site*Tillage) was significant only on TCR and ACR. The mean colonization rates were 31.6, 27.9 and 10.2% for TCR, ACR and VCR, successively. For both Tilled (T) and no-tilled (NT) plots, the TCR recorded in the site “Station” was higher than TCR in “Benifouda” by 59% and TCR in “Remada” by 74%, while the TCR recorded under NT system over all sites was 54% higher than TCR under T system.

Table 3: Means of total colonization rate (TCR), arbuscular colonization rate (ACR), vesicular colonization rate (VCR) and spore density (SD spores/g soil) in each plot and the significance effect of site, tillage system and their interaction site* tillage.

Root colonization (RC) depends on the density and affinity of AMF and the host plant, as well as the environmental variables such as soil characteristics and agricultural practices (Gafur, 2014). In our study, RC was positively correlated with organic matter (OM) and pH, and negatively correlated with the available phosphorus amounts (P) in the soil (Table 4). The relation between AMF and OM seems to be synergetic. AMF contribute to the increase of OM in the soil by the AMF biomass generated from the carbon translocated from the host plant, which can reach 20% of the assimilated carbon (Graham, 2000), the microbiome biomass often associated with AMF (Turrini et al., 2018) and the increase of root biomass as a result of promoting plant growth. On the other hand, organic amendments (compost or manure) stimulate AMF development (Buto et al., 2016). In contrast to OM, the available P in the soil had a negative correlation with RC. Since it is the main element enhanced by AMF, P excess in soil-root interface or in plant organs can be detected by the host plant, which reacts by inhibiting RC and/or its progress (Ferrol et al., 2019). While the positive correlation of pH with RC could be explained by the decrease in P availability due to a rise in pH (Hopkins and Ellsworth, 2005).

Table 4: Correlations between root colonization rates, spore density and soil characteristics.

Besides soil characteristics, agricultural practices also influence arbuscular mycorrhizal colonization. In general, intensive farming practices have a negative effect on AMF, in contrast to conservation agriculture practices (Säle et al. 2015). Tillage is important in the intensive crop system with many benefits. However, it can alter the physico-chemical and biological properties of the soil, including AMF (Siddiqui and Pichtel, 2008). Our findings confirmed the negative impact of tillage on AMF, where the no-tillage improved RC by 54% in durum wheat roots. These results are in accordance with the findings of Taibi et al., (2020) in similar edaphic and climatic conditions. Tillage affects RC in different ways, as turning over the soil, it buries propagules and/or exposes them to environmental stresses, which will dilute and destroy propagules, and consequently reduce the inoculum potential of the soil. By burying propagules below the depth of early seedling root growth, it delays colonization (Kabir, 2005). Kabir (2005) also speculated that in NT system, the new formed roots grow following the channels left by the old colonized roots that contains more propagules. The decrease of OM by tillage (Oehl et al. 2004), could also affect AMF development.

Root colonization of durum wheat in the irrigated site was higher than those in sites under rain-fed conditions. This suggests that water deficit may affect RC as it was reported by Mathur et al. (2018). Augé (2001), based on a literature review has concluded that long soil drought decreases RC. Drought can reduce the primary colonization by limiting spore germination (Wu and Zou, 2017), as it can affect the secondary colonization by inhibiting extra radical hyphal length (Neumann et al., 2009). In addition, RC could be affected by the carbon supply decrease, because of drought stress on the host plant growth.

Besides their dependence on the AMF species (Smith and Read, 1997), the storage character of the vesicles suggests that VCR indicates the amount of carbon transferred to AMF by the host plant (Busby et al. 2012). This can explain the high VCR at the irrigated site, where plants had a better nutritional status than sites exposed to drought.

Moreover, the improvement of RC by no-tillage varied between sites, particularly the arbuscular form (ACR) that ensures nutrients exchange (Hause and Fester, 2005). This suggests that NT effect depend also on other factors. In the irrigated site (Station), NT improved ACR by 4.76% while in Benifouda and Remada, the improvements were 75.41 and 154.95%, respectively. These results indicate that the NT effect was more important under drought conditions. The increase of water storage in the soil under no-tillage condition (Huang et al., 2012) could reduce drought impact on RC. In addition, the interesting effect of NT in Remada could be the result of the combined outcome of drought, the tillage practice and the bare fallow (preceding wheat culture), where the removal of host plants reduced arbuscular mycorrhizal establishment (Schipanski et al., 2014). Bowles et al., (2017) concluded from a meta analysis study that continuous ground cover by crops was as important as the decreasing soil disturbance for AMF.

Spore density (SD), ranged from 3.97 spores/g of soil in the plot under T system in “Benifouda” to 8.2 spores/g soil under T system in “Remada”. Unlike root colonization, tillage effect on SD was not significant despite the decrease by 6,76% (Table 3). NT usually increases spores abundance in soil (Lehman et al., 2019), which is important particularly for annual crops (Yang et al., 2014). On the other hand, the site effect was significant on SD (Table 3) with higher density in sites under-fed conditions. This could be explained by the induction of sporulation when soil moisture decreased, which is a specific characteristic allowing AMF adaption to arid conditions (Jacobson, 1997).
AMF root colonization in durum wheat varied according to site and tillage system, while spore density varied according to site. Drought seems to stimulate sporulation but reduces root colonization. No-tillage improved root colonization rates by 54% compared to conventional tillage and this improvement was higher under drought, particularly the arbuscular form. Moreover, root colonization rates showed a positive correlation with the organic matter content and pH in the soil and a negative correlation with the available phosphorus.

The positive association of no-tillage and AMF symbiosis and thus their positive effects, encourages their combination in a sustainable approach to produce durum wheat under semi-arid conditions.

  1. Anne, P. (1945). Sur le dosage rapide du carbone organique des sols. Annales Agronomiques. 2(1): 161-172.

  2. Augé, R.M. (2001). Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza. 11(1): 3-42.

  3. Bowles, T.M., Jackson, L.E., Loeher, M. and Cavagnaro, T.R. (2017). Ecological intensification and arbuscular mycorrhizas: A meta-analysis of tillage and cover crop effects. Journal of Applied Ecology. 54(6): 1785-93. 

  4. Bremner, J.M. and Mulvaney, C.S. (1982). Total Nitrogen. In: Methods of Soil Analysis. Part 2. [A.L. Page, R.H. Miller and D.R. Keeney (Eds)]. American Society of Agronomy. Madison, WI USA. 595-624.

  5. Busby, R.R., Paschke, M.W., Stromberger, M.E. and Gebhart, D.L. (2012). Seasonal variation in arbuscular mycorrhizal fungi root colonization of cheatgrass (Bromus tectorum): An invasive winter annual. Journal of Ecosystem and Ecography. S8: 001. doi:10.4172/2157-7625.S8-001.

  6. Buto, T., Suzuki, K., Kaidzu, T., Narisawa, T., Turgay, O.C., Ortas, I., Harada, N. and Nonaka, M. (2016). Arbuscular mycorrhizal fungal community of wheat under long-term mineral and organic amendments in semi-arid Mediterranean Turkey. Arid Land Research and Management. 30(4): 479-489.

  7. Chen, H., Hou, R., Gong, Y., Li, H., Fan, M. and Kuzyakov, Y. (2009). Effects of 11 years of conservation tillage on soil organic matter fractions in wheat monoculture in Loess Plateau of China. Soil and Tillage Research. 106(1): 85-94.

  8. Ferrol, N., Azcón-Aguilar, C. and Pérez-Tienda, J. (2019). Arbuscular mycorrhizas as key players in sustainable plant phosphorus acquisition: An overview on the mechanisms involved. Plant Science. 280: 441-447.

  9. Gafur, S. (2014). Contribution of Dynamics of Root Colonisation by Arbuscular Mycorrhizal Communities to Ecosystem Function. In: Mycorrhizal Fungi: Use in Sustainable Agriculture and Land Restoration. Springer, Berlin, Heidelberg. 33-43.

  10. Gerdemann, J.W. and Nicolson, T.H. (1963). Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Transactions of the British Mycological Society. 46: 235-244.

  11. Graham, J.H. (2000). Assessing costs of Arbuscular Mycorrhizal Symbiosis in Agroecosystems. In: Current Advances in Mycorrhizae Research. In: Current Advances in Mycorrhizae Research. [Podila G.K., Douds D.D. (eds)]. St Paul, MN, APS Press. USA: 127-140. 

  12. Hause, B. and Fester, T. (2005). Molecular and cell biology of arbuscular mycorrhizal symbiosis. Planta. 221(2): 184- 196.

  13. Hopkins, B. and Ellsworth, J. (2005). Phosphorus Availability with Alkaline/calcareous Soil. Western Nutrient Management Conference. 6: 88-93. 

  14. Horneck, D.A., Sullivan, D.M., Owen, J.S. and Hart, J.M. (2011). Soil Test Interpretation Guide. Oregon State University, Extension Service.

  15. Huang, G.B., Chai, Q., Feng, F.X. and Yu, A.Z. (2012). Effects of different tillage systems on soil properties, root growth, grain yield, and water use efficiency. .Journal of Integrative Agriculture. 11(8): 1286-1296

  16. Jacobson, K.M. (1997). Moisture and substrate stability determine VA-mycorrhizal fungal community distribution and structure in an arid grassland. Journal of Arid Environments. 35(1): 59-75.

  17. Kabir, Z. (2005). Tillage or no-tillage: Impact on mycorrhizae. Canadian Journal of Plant Science. 85(1): 23-29.

  18. Lehman, R.M., Osborne, S.L., Taheri, W.I., Buyer, J.S. and Chim, B.K. (2019). Comparative measurements of arbuscular mycorrhizal fungal responses to agricultural management practices. Mycorrhiza. 29(3): 227-235.

  19. Marandola, D., Belliggiano, A., Romagnoli, L. and Ievoli, C. (2019). The spread of no-till in conservation agriculture systems in Italy: Indications for rural development policy-making. Agricultural and Food Economics. 7(1): 1-22.

  20. Mathur, S., Tomar, R.S. and Jajoo, A. (2019). Arbuscular mycorrhizal fungi (AMF) protects photosynthetic apparatus of wheat under drought stress. Photosynthesis Research. 139(1): 227-238.

  21. McGonigle, T.P., Miller, M.H., Evans, D.G., Fairchild, G.L. and Swan, J.A. (1990). A new method which gives an objective measure of colonization of roots by vesicular arbuscular mycorrhizal fungi. New Phytologist. 115: 495-501.

  22. Neumann, E., Schmid, B., Römheld, V. and George, E. (2009). Extraradical development and contribution to plant performance of an arbuscular mycorrhizal symbiosis exposed to complete or partial root zone drying. Mycorrhiza. 20(1): 13-230.

  23. Oehl, F., Sieverding, E., Mader, P., Dubois, D., Ineichen, K., Boller, T. and Wiemken, A. (2004). Impact of long-term conventional and organic farming on the diversity of arbuscular mycorrhizal fungi. Oecologia. 138: 574-583.

  24. Olsen, S.R. (1954). Estimation of Available Phosphorus in Soils by Extraction with Sodium Bicarbonate. (No. 939). US Department of Agriculture.

  25. Phillips, J.M. and Hayman, D.S. (1970). Improved procedures for clearing roots and staining parasitic and vesicular- arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society. 55(1): 158-161.

  26. Rouabhi, A., Laouar, A., Mekhlouf, A. and Dhehibi, B. (2018a). What are the factors affecting no-till adoption in the farming system of Setif province in Algeria? Turkish Journal of Agriculture - Food Science and Technology. 6(6): 636-641. 

  27. Rouabhi, A, Kebiche, M. and M Hafsi. (2018b). Climate Change during the last century in Sétif Province. Agriculture. 8(2): 61-75.

  28. Säle, V., Aguilera, P., Laczko, E., Mäder, P., Berner, A., Zihlmann, U. and Oehl, F. (2015). Impact of conservation tillage and organic farming on the diversity of arbuscular mycorrhizal fungi. Soil Biology and Biochemistry. 84: 38-52.

  29. Schipanski, M.E., Barbercheck, M., Douglas, M.R., Finney, D.M., Haider, K., Kaye, J.P. and White, C. (2014). A framework for evaluating ecosystem services provided by cover crops in agroecosystems. Agricultural Systems. 125: 12-22.

  30. Siddiqui, Z.A. and Pichtel, J. (2008). Mycorrhizae: An Overview. In Mycorrhizae: Sustainable Agriculture and Forestry. Springer, Dordrecht. 1-35.

  31. Smith, SE. and Smith, FA. (2011). Roles of Arbuscular mycorrhizas in plant nutrition and growth: New paradigms from cellular to ecosystem scales. Annual Review of Plant Biology. 62: 227-250.

  32. Smith, S. and Read, D. (1997). Mycorrhizal symbiosis (Academic, San Diego). SmithMycorrhizal Symbiosis. 1997.

  33. Taibi, H.H.Y., Smail-Saadoun, N., Labidi, S., Abdellaoui, K., Makhlouf, M., Laouar, A., Benouaret, C., Rezki-Sekhi, L., Boukais, A.B. and Sahraoui, A.L.H. (2020). The influence of no-till farming on durum wheat mycorrhization in a semi-arid region: A long-term field experiment. Journal of Agricultural Science. 12(4). doi: 10.5539/jas.v12n4p77.

  34. Thirkell, T.J., Charters, M.D., Elliott, A.J., Sait, S.M. and Field, K.J. (2017). Are mycorrhizal fungi our sustainable saviours? Considerations for achieving food security. Journal of Ecology. 105(4): 921-29.

  35. Turrini, A., Avio, L., Giovannetti, M. and Agnolucci, M. (2018). Functional complementarity of arbuscular mycorrhizal fungi and associated microbiota: The challenge of translational. Frontiers in Plant Science. 9: 1407.

  36. Wu, Q.S. and Zou, Y.N. (2017). Arbuscular Mycorrhizal Fungi and Tolerance of Drought Stress in Plants. In Arbuscular Mycorrhizas and StressTolerance of Plants. Springer, Singapore. 25-41. 

  37. Yang, C., Ellouze, W., Navarro-Borrell, A., Esmaeili Taheri, A., Klabi, R., Dai, M., Kabir, Z. and Hamel, C. (2014). Management of the arbuscular mycorrhizal symbiosis in sustainable crop production. Mycorrhizal fungi: Use in sustainable agriculture and land restoration. Soil Biology. 41: 89-113.

  38. Zhang, S., Lehmann, A., Zheng, W., You, Z. and Rillig, M.C. (2019). Arbuscular mycorrhizal fungi increase grain yields: A meta analysis. New Phytologist. 222(1): 543-555.

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