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Current IssueEditorial BoardOnline First Articles
Agricultural Science Digest
Print ISSN: 0253-150X
Online ISSN: 0976-0547
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Chief Editor:
Arvind kumar
Rani Lakshmi Bai Central Agricultural Uni., Jhansi, U.P., INDIA
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Research Article

Agricultural Science Digest, volume 41 special issue (april 2021) : 186-190

Isolation and Identification of the Fungi Causing Tomato Fruit Rot Disease in the Vicinity of Tandojam, Sindh

S. Nizamani, A.A. Khaskheli, A.M. Jiskani, S.A. Khaskheli, A.J. Khaskheli, G.B. Poussio, H. Jamro, M.I. Khaskheli
1Department of Plant Protection, Sindh Agriculture University, Tandojam-70051, Pakistan.
Cite article:- Nizamani S., Khaskheli A.A., Jiskani A.M., Khaskheli S.A., Khaskheli A.J., Poussio G.B., Jamro H., Khaskheli M.I. (2020). Isolation and Identification of the Fungi Causing Tomato Fruit Rot Disease in the Vicinity of Tandojam, Sindh. Agricultural Science Digest. 41(2021): 186-190. doi: 10.18805/ag.D-269.

ABSTRACT

Background: The post-harvest tomato fruit rot disease is common threat to the tomato fruit, causing huge economic loss as revealed by (GOP, 2018). The present study was conducted for isolation and identification of causative agent of tomato fruit rot in order to formulate the proper management stretegies. 
Methods: Study was conducted in three phases. Phase one included collection of tomato fruit samples from vicinity of Tandojam. In phase two pathogens were isolated from the samples at laboratory, while in the phase three pathogens were identified using standard procedures. 
Result: The experimental results indicated Alternaria solani as the main cause of post-harvest tomato fruit rot. The symptoms observed were presence of brown to black rot lesions on tomato fruits with distinct rings ranging from small pin-heads to whole surface of fruit. A total of six different fungi viz., Alternaria alternata, Aspergillus niger, Alternaria solani, Geotrichum candidum, Fusarium oxysporum and Rhizopus stolonifer were found to be associated with post harvest tomato rot. Significantly higher infection was recorded for A. solani (53.667%) followed by A. niger (16.333%) and G. candidum (13.00%). The lowest infection percentage was observed for F. oxysporum (2.333%), followed by A. alternata (4.00%) and R. stolonifer (9.00%). A. solani produced aerial mycelium with yellowish to reddish diffusible pigments. A. niger cultures were typically black and colonies were initially whitish to yellow and later became brown to black in colour. G. candidum produced white and nonaerial colonies. F. oxysporum produced circular, aerial mycelium initially white, later changed to light pink. R. stolonifer produced whitish to grey fuzzy colonies. 

REFERENCES

  1. Abdel-Kader, M., El-Mougy, N. and Lashin, S. (2011). Evaluation of grapefruit coating with chemical preservatives as control measure against postharvest decay. Phytopathologia. 59: 25-38.
  2. Adekalu, O.A., Olatunde, I.G. Echendu, B.M. Adepoju, T.C. and Fajemisin O.O. (2009). Antimicrobial and preservative activities of Allium sativum and Eugenia aromatica on fresh tomato puree. African Journal of Agricultural Research. 4: 139-140. 
  3. Awan, S.M., Hussain, A., Abbas T. and Karim, R. (2012). Assessment of production practices of small scale farm holders of tomato in Bagrote Valley, CKNP region of Gilgit-Baltistan, Pakistan. Slovenica. 99: 191-199. 
  4. Ayala-Zavala, J.F. and Gonzlez-Aguilar, G.A. (2010). Optimizing the use of garlic oil as antimicrobial agent on fresh-cut tomato through a controlled release system. Journal of Food Science. 75: 398-405.
  5. Barnett, H.L. and Hunter, B. (1972). Illustrated genra of imperfect fungi. 3rd ed. Burgress Publishing Company Minneapolis. 
  6. Bashir, U., Mushtaq S. and Akhtar, N. (2014). First report of Alternaria metachromatica from Pakistan causing leaf spot of tomato. Pakistan Journal of Agriculture Science. 51: 305-308.
  7. Britt, B.F. and Kristin, R. (2011). Tomato consumption and health: Emerging benefits. Amarican Journal of Life Style Medicine. 81: 2-191. 
  8. Charchar, A.U., Gonzaga, J.M., Giordano, V., Boiteuy, L.de. and Reis, L.S. (2003). Reaction of tomato cultivars to infection by a mixed population of M. incognita race and M. javanica in the field. Nematol. Brasileira. 27: 49-54.
  9. Chohan, S., Perveen, R., Mirza, A. and Mahmood, A.R. (2016). Fungi colonizing different parts of tomato plant (Lycopersicon lycopersicum L.). Journal of Phytopathology. 28: 25-33.
  10. GOP. (2018). Agriculture statistics of Pakistan. Govt of Pakistan, Area, production of fruit, vegetables and Condiments. Ministry of Food and Agriculture (Economic wing). 2010-11.
  11. Gomez, K. and Gomez, A.A. (1984). Statistical Procedure for the Agriculture Research. Wily and Sone.
  12. Hassan, H.A.H. (1996). Alternaria mycotoxins in Black rot lesion of tomato fruits: Conditions and regulations of their productions. Acta Immunolgica Hungarica. 43:125-133.
  13. Kirk, P.M., Cannon, P.F., Minter, D.W. and Stalpers, J.A. (2008). Dictionary of the Fungi. 10th ed., Wallingford, CABI, 22.
  14. Mbuk, E.M., Bassey, N.E., Udoh, E.S. and Udoh, E.J. (2011). Factors influencing postharvest loss of tomato in Uyo Urban market in Uyo Nigeria. Journal of Agriculture Food Environment. 7: 40-46.
  15. Mujib, U.R., Naushad, K. and Inayatullah, J. (2007). Post-harvest losses of tomato crop. Sarhad Journal of Agriculture. 23: 1279-1284. 
  16. Nasir, M.U., Sarfraz, H. and Saqib, J. (2015). Tomato processing, lycopene and health benefits: A review. Science Letters. 3: 1-5.
  17. Nelson, P.E., Toussoun, T.A. and Marasas, W.F.O. (1983). Fusarium species. An illustrated manual of identification. The State Univ. Press, University Park, Pennslyvania.
  18. Nowicki, M., Nowakowska, M., Niezgoda, A. and Kozik, E.U. (2012). Alternaria black spot of crucifers: symptoms, importance of disease and perspectives of resistance breeding vegetable crops. Research Bulletin. 76: 5-19.
  19. Obetta, S.E, Nwakonobi, T.U. and Adikwu, O.A. (2011). Microbial effects on selected stored fruits and vegetables under Ambient Makurdi, Benue State, Nigeria. Research Journal of Applied Sciences, Engineering and Technology. 3: 393-398.
  20. Pandey, A.K., Kumar, P., Singh, P., Tripathi, N.N. and Bajpai, V.K. (2017). Essential Oils: Sources of Antimicrobials and Food Preservatives. Frontiers in Microbiology. 7: 2161-2168.
  21. Sajad, A.M. and Jamaluddin Abid, H.Q. (2017). Fungi associated with the spoilage of post harvest tomato fruits in different markets of Jabalpur, Madhya-Pradesh, India. International Journal of Current Research Review. 9: 12-16.
  22. Thirupathi, V., Sasikala, S. and Kennedy, J.Z. (2006). Preservation of fruits and vegetables by wax coating. Sci. Tech. Entrepreneur. 22: 19-24. 
  23. Wani, A.H. (2011). An overview of the fungal rot of tomato. Mycopathology. 9: 33-38.
  24. Wee, R.Y. (2017). The economies of Southeast Asian nations. Worldatlas.
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