Relationship between In vivo, In vitro Parameters and Chemical Composition to Predict the Nutritive Value of Some Legume Forages

DOI: 10.18805/ag.D-252    | Article Id: D-252 | Page : 295-300
Citation :- Relationship between In vivo, In vitro Parameters and Chemical Composition to Predict the Nutritive Value of Some Legume Forages.Agricultural Science Digest.2021.(41):295-300
A. Aïssa, C. Ben Mustapha, M.R. Alvir, M. Hadj Ayed, I.E.A. Znaïdi, N. Moujahed, K. Kraiem aissaasma20147@gmail.com
Address : Département de Ressources Animales, Agroalimentaires et Développement Rural, Institut Supérieur Agronomique de Chott-Mariem, BP 47, 4042 Chott-Mariem. Université de Sousse, Tunisie.
Submitted Date : 30-03-2020
Accepted Date : 7-11-2020


Background: Many in vivo and laboratory methods have been used to evaluate ruminant feeds. The objective of this experiment was to determine feed intake, digestibility, kinetics of gas production, in vitro organic matter digestibility (OMDv) and metabolizable energy (ME) of Trifolium alexandrinum (T. alexandrinum) and Medicago sativa (M. sativa) at different maturity stages through in vivo and in vitro methods to elaborate predictive equations from chemical constituents. 
Methods: In vivo assay was carried out with two groups of five rams male kipped in metabolism cage. Samples of forage, refusal and feces were collected and processed for chemical analysis. In vitro gaz production technique was performed on forage samples.
Result: The bud and early bloom stage of M. sativa recorded the highest digestibility values of the chemical component (P<0.05). The OMDv of M. sativa was 60.65 and 68.26% (P<0.01) for early bloom stage and bud stage, respectively. Crude protein digestibility (CPD) was positively correlated with crude protein rate (R2=0.83, P<0.05). The gas production from the insoluble fraction “b” fraction showed a positive correlation with acid detergent fiber (ADF) (R2=0.999, P<0.001). However, cumulative gas production at 24h and 48 h of incubation were negatively correlated (P<0.05) with the ADF rate (R2= -0.98 and -0.97, respectively). 


Chemical composition Digestibility Green forages Gas production Maturity stage Prediction equations


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