Agricultural Science Digest

  • Chief EditorArvind kumar

  • Print ISSN 0253-150X

  • Online ISSN 0976-0547

  • NAAS Rating 5.52

  • SJR 0.156

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Agricultural Science Digest, volume 40 issue 1 (march 2020) : 84-88

Platelet Rich Plasma in the Treatment of Superficial Digital Flexor Tendon Core Lesion in Donkeys: Histopathological Assessment

Mohamed Mostafa, Ahmed Khalil, Adel Al-Akraa
1Department of Veterinary Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. 
Cite article:- Mostafa Mohamed, Khalil Ahmed, Al-Akraa Adel (2020). Platelet Rich Plasma in the Treatment of Superficial Digital Flexor Tendon Core Lesion in Donkeys: Histopathological Assessment. Agricultural Science Digest. 40(1): 84-88. doi: 10.18805/ag.D-198.
The aim of this study was to assess the effect of platelet rich plasma (PRP) on the healing of created core lesions in the superficial digital flexor tendon (SDFT) in donkeys. Serial histopathological specimens were taken from normal and at 5th, 15th, 30th, 60th and 90th days post PRP treatment. Histopathological findings showed early rapid healing process with early angiogenesis, increased fibroblastic condensation, the nuclei appeared more elongated, flattened arranged in the peripheral collagen fibers. The collagen fibers changed from randomly arranged to semi-parallel and finally highly reoriented parallel closely packed collagen fibers at 90th days of PRP treatment. Compared to saline control displayed few scattered fibroblasts and angiogenesis. As well as less regularly arranged collagen fibers. Histopathological evaluation 90 days after three successive PRP treatment proved a more rapid tendon tissue reconstruction and maturation similar to the healthy tendons. 
  1. ALzola A.R., Easter C., Rigs C.M., Gardner D.S., Freeman S.L. (2018). Ultrasonographic-Based predictive factors influencing successful return to racing after superficial digital flexor tendon injuries in flat racehorses: A retrospective cohort study in 469 Thoroughbred racehorses in Hong Kong. Equine Veterinary Journal. 50: 602-608.
  2. Arguelles D., Carmona J., Climent F., Munoz E. and Prades M., (2008). Autologous platelet concentrates as a treatment for musculoskeletal lesions in five horses. Veterinary Record. 162: 208-211.
  3. Bosch G. (2009). Towards a staged evidence-based approach for the treatment of tendon injuries in the horse. PhD thesis, Faculty of Veterinary Medicine. Utrecht University.
  4. Bosch G., van Schie H., de Groot M., Cadby J., van de Lest C., Barneveld A., van Weeren R. (2010). Effects of platelets rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendon: A placebo-controlled experimental study. Journal of Orthopedic Research. 28: 211-217. 
  5. Bosch G., van Weeren R., Barneveld A., van Schie H. (2011). Computerised analysis of standardi sedultra sonographic images to monitor the repair of surgically created core lesions in equine Superficial digital flexor Tendon following treatment with intratendinous Platelets Rich Plasma or placebo. The Veterinary Journal.187: 92–98.
  6. Brandão J.S., Alvarenga M.L., Pfeifer J.P.H., dos Santos V.H., Fonseca Alves C.E., et al. (2018). Allogeneic mesenchymal stem cell transplantation in healthy equine superficial digital flexor tendon: A study of the local inflammatory response. Research in Veterinary Science. 118: 423- 430.
  7. Carmona J.U., Gómezn W.A., López C. (2018). Could Platelet    Rich Plasma be a clinical treatment for horses with laminitis? Journal of Equine Veterinary Science. 61: 46–57.
  8. Dyson S. (2004). Medical management of SDF tendonitis: a comparative study in 219 horses (1992-2000). Equine Veterinary Journal. 36: 415-419.
  9. Geburek F., Gaus M., van Schie H.T., Rohn K., Stadler P.M. (2016). Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies - a randomized prospective controlled clinical trial. BMC Veterinary Research. 12(1): 816–826.
  10. Geburek F., Roggel F., van Schie H.T.M., Beineke A., Estrada R., Weber K., Hellige M., Rohn K., et al. (2017). Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions withadipose- derived mesenchymal stromalcells: a controlled experimental trial. Stem Cell Research and Therapy. 8: 129-140.
  11. Kaneps A.J. (2016). Therapies for equine soft tissue injuries. Proceedings American Association. Equine Practice. Congress. Orlando USA, 62 (3–7) December: 241–244.
  12. Kwirant L.A.A., De La Corte F.D., Cantarelli C., Cargnelutti J.F., Martins M., Cabral M.W., Maciel N., Rubin M.IB. (2019). Cooling and Cryopreservation of Equine Platelet-Rich Plasma with Dimethyl Sulfoxide and Trehalose. Journal of Equine Veterinary Science. 72: 112-116.
  13. Maia, M.V. de Souza, J.I. Ribeiro Júnior, A.C. de Oliveira, G.E.S. Alves, L. dos Anjos Benjamin, Y.F.R.S. Silva, B.M. Zandim, Jd.C.L. Moreira (2018). Platelet-rich plasma in the treatment of induced tendinopathy in horses: histologic evaluation. Journal of Equine Veterinary Science. 29(8): 618–626.
  14. Hall L, Clarke K and Trim A (2001): Veterinary anesthesia 3rd ed. W.B Saunders, 90-250.
  15. Mazzocca A.D., McCarthy M.B., Intravia J., Beitzel K., Apostolakos J., Cote M.P., et al. (2013). An in vitro evaluation of the anti-inflammatory effects of platelet-rich plasma, ketorolac and Methyl prednisolone. Arthroscopy. 29(4): 675–83.
  16. Miranda A.L., Blanco B.S., Lopes P.L., Raphael M., Palhares V.M.S. (2018). Influence of anti-coagulants on platelet and leukocyte concentration from platelet-rich plasma derived from blood of horses and mules. Journal of Equine Veterinary Science. 63: 46-50.
  17. Molloy T., Wang Y., Murrell G. (2003).The roles of growth factors in tendon and ligament healing. Sports Medicine. 33(5): 381–894.
  18. Mostafa M.B., Al-Akraa A.M., Khalil A.H. (2015a). Clinical assessment of superficial digital flexor tendon (SDFT) core lesion treated with Platelet Rich Plasma (PRP) in donkeys (Equus asinus). International Journal of Advanced Research. 3(10): 371-381.
  19. Mostafa M.B., Al-Akraa A.M., Khalil A.H. (2015b). Ultrasonographic assessment of superficial flexor tendon (SDFT) core lesion treated with Platelet Rich Plasma (PRP) in donkeys. Benha Veterinary Medical Journal. 5: 274-282.
  20. Schramme M., Hunter S., Campbell N., Blikslager A. Smith R. (2010). A surgical tendonitis model in horses: technique, clinical, ultrasonographic and histological characterization. Veterinary and Comparative Orthopeadic Traumatology. 23: 231-239. 
  21. Textor J.A., Tablin F. (2013). Intra-articular use of a platelet-rich product in normal horses: clinical signs and cytologic responses. Veterinary Surgery. 42(5): 499–510.
  22. Tsukiyama K., Acorda J., Yamada H. (1996). Evaluation of SDF tendinitis in racing horses through grey-scale histogram analysis of tendon ultrasonograms. Veterinary Radiology and Ultrasound. 37(18): 46-50. 
  23. Zhang J., Middleton K., Fu F., Hee-Jeong, Wang J. (2013). Do HGF Mediates the anti-inflammatory effects of Platelets Rich Plasma on injured tendons. PLoS ONE. 8(6): e67303. doi:10.1371/journal.pone.0067303 8 (6): 232-240.
  24. Zuffova K., Krisova S., Zert Z. (2013). Platelet rich plasma treatment of superficial digital flexor tendon lesions in racing thoroughbreds. Veterinarni Medicine. 58(4): 230-239. 

Editorial Board

View all (0)