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Current IssueEditorial BoardOnline First ArticlesArchive

volume 34 issue 4 (december 2014) : 273 - 276,   Doi: 10.5958/0976-0547.2014.01018.0

EXTRACTION TECHNIQUES OF PLANT-PARASITIC NEMATODES : A PRACTICAL COMPARISON

H
Homan Regmi*
A
Ajit Kumar Ngangbam1
B
Bijayalakshmi Devi Nongmaithem2
1Biotechnology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur, Nepal
Cite article:- Regmi* Homan, Ngangbam1 Kumar Ajit, Nongmaithem2 Devi Bijayalakshmi (2025). EXTRACTION TECHNIQUES OF PLANT-PARASITIC NEMATODES : A PRACTICAL COMPARISON. Agricultural Science Digest. 34(4): 273 - 276. doi: 10.5958/0976-0547.2014.01018.0.

ABSTRACT

The efficiency of various nematode extraction methods differs with the time period, equipments used, soil type, moisture content of the soil, different nematode genera with different life stages, and basically upon the performance of the individual (s) who is carrying out the extraction. The present study was conducted in the Institute for Agriculture and Fisheries Research (ILVO), Gent University, Belgium to study the efficiency of different nematode extraction methods and to extract nematodes from soil, root and soil with root samples. From the present study, it was observed that the Seinhorst method is better for extracting both lighter and heavier cysts nematodes even though it needs more expensive equipments and much expert to run as compared to stirring method. Automotive Zonal Centrifugation (AZC) method was found more efficient and rapid technique than Cobb’s method in extracting mobile and non mobile nematodes from soil. Baermann funnel is suitable for the extraction of the mobile nematodes and found not to be suitable to explore for quantitative research.

REFERENCES

  1. Barker, K. R. and Campbell, C.L. (1981). Sampling nematode populations. In: Zuckerman, B.M., Rohde, R.A. (Eds.), Plant Parasitic Nematodes, vol. 3. Academic Press, New York. 451–474.
  2. McSorley, R. and Parrado, J.L. (1987). Nematode losses during centrifugal extraction from two soil types. Nematropica 17, 147–161.
  3. McSorley, R  and Frederick, J.F. (2004). Effect of extraction method on perceived composition of the soil nematode community. Applied Soil Ecology 27: 55-63.
  4. McSorley, R and Walter, D.E, (2008). Comparison of soil extraction methods for nematodes and microarthropods. African Journal of Microbiology Research 2: 167-170.
  5. Van Bezooijen J. (2006). Methods and Techniques for Nematology Manual. 118 pp. The Netherlands.
  6. Viglierchio, D.R. and  Schmitt, R.V. (1983). On the methodology of nematode extraction from field samples: Baermann funnel modifications. J Nematol. 15(3): 450–454.
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    volume 34 issue 4 (december 2014) : 273 - 276,   Doi: 10.5958/0976-0547.2014.01018.0

    EXTRACTION TECHNIQUES OF PLANT-PARASITIC NEMATODES : A PRACTICAL COMPARISON

    H
    Homan Regmi*
    A
    Ajit Kumar Ngangbam1
    B
    Bijayalakshmi Devi Nongmaithem2
    1Biotechnology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur, Nepal
    Cite article:- Regmi* Homan, Ngangbam1 Kumar Ajit, Nongmaithem2 Devi Bijayalakshmi (2025). EXTRACTION TECHNIQUES OF PLANT-PARASITIC NEMATODES : A PRACTICAL COMPARISON. Agricultural Science Digest. 34(4): 273 - 276. doi: 10.5958/0976-0547.2014.01018.0.

    ABSTRACT

    The efficiency of various nematode extraction methods differs with the time period, equipments used, soil type, moisture content of the soil, different nematode genera with different life stages, and basically upon the performance of the individual (s) who is carrying out the extraction. The present study was conducted in the Institute for Agriculture and Fisheries Research (ILVO), Gent University, Belgium to study the efficiency of different nematode extraction methods and to extract nematodes from soil, root and soil with root samples. From the present study, it was observed that the Seinhorst method is better for extracting both lighter and heavier cysts nematodes even though it needs more expensive equipments and much expert to run as compared to stirring method. Automotive Zonal Centrifugation (AZC) method was found more efficient and rapid technique than Cobb’s method in extracting mobile and non mobile nematodes from soil. Baermann funnel is suitable for the extraction of the mobile nematodes and found not to be suitable to explore for quantitative research.

    REFERENCES

    1. Barker, K. R. and Campbell, C.L. (1981). Sampling nematode populations. In: Zuckerman, B.M., Rohde, R.A. (Eds.), Plant Parasitic Nematodes, vol. 3. Academic Press, New York. 451–474.
    2. McSorley, R. and Parrado, J.L. (1987). Nematode losses during centrifugal extraction from two soil types. Nematropica 17, 147–161.
    3. McSorley, R  and Frederick, J.F. (2004). Effect of extraction method on perceived composition of the soil nematode community. Applied Soil Ecology 27: 55-63.
    4. McSorley, R and Walter, D.E, (2008). Comparison of soil extraction methods for nematodes and microarthropods. African Journal of Microbiology Research 2: 167-170.
    5. Van Bezooijen J. (2006). Methods and Techniques for Nematology Manual. 118 pp. The Netherlands.
    6. Viglierchio, D.R. and  Schmitt, R.V. (1983). On the methodology of nematode extraction from field samples: Baermann funnel modifications. J Nematol. 15(3): 450–454.
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