AGROBACTERIUM RHIZOGENE MEDIATED TRANSFORMATION OF NIGER [GUIZOTIA ABYSSINICA (L.F.) CASS.]

Article Id: ARCC925 | Page : 38 - 39
Citation :- AGROBACTERIUM RHIZOGENE MEDIATED TRANSFORMATION OF NIGER [GUIZOTIA ABYSSINICA (L.F.) CASS.].Agricultural Science Digest.2011.(31):38 - 39
B.T. Dangat and A.R. Patil
Address : Department of Botany, Rajaram College, Kolhapur - 416 004, India.

Abstract

A protocol was developed for Agrobacterium rhizogene mediated transformation of Niger (Guizotia abyssinica (L.f.) Cass.). Co-cultivation of  cotyledons  from one week old plantlets with Agrobacterium rhizogene strain  ATCC15834  induces negatively geotropic hairy roots directly from explants without intermediate stage i.e. callus on MS medium,  supplemented with 1 mg/l of  6-Benzyl amino purine (BAP). Transformation was confirmed on the basis of formation of negatively geotropic hairy roots compared with control.

Keywords

Agrobacterium rhizogene Transformation Niger.

References

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