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BIOSYNTHESIS PATHWAYS OF IAA PRODUCTION IN ACINETOBACTER HAEMOLYTICUS

Article Id: ARCC643 | Page : 214-218
Citation :- BIOSYNTHESIS PATHWAYS OF IAA PRODUCTION IN ACINETOBACTER HAEMOLYTICUS.Agricultural Science Digest.2012.(32):214-218
S.D. Bhawsar*, S.D. Patil and B.A. Chopade dr.sonalibhawsar@indiatimes.com
Address : Department of Microbiology University of Pune,Pune-411 007, India

Abstract

Acinetobacter haemolyticus, A19 produced IAA in presence of different wheat root exudates and also in absence of standard precursor tryptophan in production medium. IAA production was increased with concentration of L-tryptophan from 50-200µg/ml in growth medium. However similar results were not obtained when indole, a structural analog of IAA was used as a precursor. HPLC analysis showed that using tryptophan and indole as precursors, IAA was synthesized by A19 by two possible routes via tryptamine and indole-3 acetonitrile as intermediates.

Keywords

Acinetobacter Root exudates Tryptophan Indole HPLC analysis.

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