The present study was conducted to test the efficacy of potential isolates of bioagents against bacterial leaf blight of rice applied as three time sequences. Application of bioagents formulation, 7 days before pathogen inoculation were found significantly effective in reducing disease severity over check and chemical treatment. Reduction in disease severity (63.19, 51.31 and 38.23%) was noticed with T. harzianum at all three time of application (7 days before, simultaneously and 7 days after pathogen inoculation, respectively). Application of bioagents 7 days before and simultaneously with pathogen inoculation was found more effective than chemical treatment in increasing grain yield. PBA-3 exhibited maximum increase (64.85%) in grain yield when applied 7 days before pathogen inoculation. Maximum increase (29.26%) in 1000 grain weight was showed by PBA-2, when applied 7 days before pathogen inoculation.