A field experiment was conducted during summer seasons of 1998 and 1999 to study the response of three root rot resistant cultures of sesame viz., ORM 7, ORM 14 and ORM 17 to three spacing (30x10, 30x20 and 30x30 cm) and three NPK levels (100, 125 and 150 per cent of the recommended dose). The pooled results revealed that among the three cultures, the culture ORM 17 recorded higher number of branches, crop drymatter production and number of capsules/plant and seed yield (691 kg/ha). A favourable increase in the yield parameters was observed with a spacing of 30x30 cm. However a spacing of 30x10 cm significantly recorded a higher seed yield of 622 kg/ha. Increasing NPK levels upto 150 per cent resulted markedly higher yield attributes and a seed yield of 630 kg/ha. Among the different combination of varieties, spacing and fertilizers, the culture ORM 17 grown under a spacing of 30 x 10 cm with 150% NPK recorded the highest seed yield of 844 kg/ha.