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EXUDATION AND BROWNING IN TISSUE CULTURE OF POMEGRANATE

Article Id: ARCC4794 | Page : 29 - 31
Citation :- EXUDATION AND BROWNING IN TISSUE CULTURE OF POMEGRANATE.Agricultural Science Digest.2003.(23):29 - 31
Ashutosh A. Murkute, Shanti Patil and Mayakumari
Address : Tissue Culture Laboratory, Department of Agricultural Botany, College of Agriculture, Nagpur- 440 001, India

Abstract

The browning of cultures is a major obstacle in establishment of explants in perennial fruit crops, which subsequently makes the in vitro techniques more cemplicated. Pomegranate Punica granatum L. has the same problem in establishment of in vitro cultures due to high phenolic contents. Several treatments were tried including use of adsorbents and antioxidants with different explants viz., leaf segment, cotyledon, nodal segment and shoot tip. The activated charcoal (adsorbent) and ascorbic acid (antioxidant) could not alter the extent of browning at various concentrations. The subculturing of explants thrice, at an interval of 24 hrs. controlled browning in all explants used, except cotyledon. The cotyledon explant was deprived of any browning secretion.

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