Wheat samples infected by leaf rust of wheat (Puccinia recondita f. sp. tritici) were collected/received from wheat growing areas of Karnataka and Maharashtra during 2000–01. The samples were analysed on set of differentials for monitoring the flora of the fungus. Pathotype 104-2 (21R55) was noticed as the most predominant in the area. Pathotype 77-5 (121R63-1) was noticed as second most predominant among virulence population. The pathotype group 104 was occurred at maximum frequency i. e. 40.4 per cent. Near isogenic lines viz., Lr 9, Lr 19 and Lr 24 were not matched by any of the isolate whereas, Lr 2c, Lr 3 and Lr 14a were completely defeated by the prevalent pathotypes. Near isogenic lines Lr 13 and Lr 23 proved to be next best effective genes after intact genes Lr 9, Lr 19 and Lr 24 in the area.