Experiment was conducted to screen different varieties and advanced lines against early blight of tomato caused by Alternaria solani. Twenty one advanced lines and five varieties were screened under field conditions. Early blight was found to be the major disease of tomato under agro-climatic conditions of Konkan region of Maharashtra State. None of the varieties and advanced lines showed resistant reaction. Five advanced lines were moderately resistant. While one variety and four advanced lines were moderately susceptible and thirteen advanced lines and four varieties were found to be susceptible to early blight of tomato.