Background: Treatment failure of multiple cases becomes a source of attention toward mycosis infections at Uttarakhand hills. Antifungals found almost ineffective against dermatophytes in several cases and their pathogenic genes (i.e SQLE), enhance drug failure due several kinds of genetic modifications. Due to a biogeography of this area prevalence of humidity is favorable climatic conditions for dermatophytes and consistent use of antifungals as well as OTC medicines became a cause of recalcitrance of pathogen. 
 
Methods: Monsoon is a favorable climate of dermatophyte in which they grow vigorously, so during this seasons (July-Oct 2024) almost 550 samples (all 5 locations) of dead stratum corneum from margins of tinea rashes were collected , heat fixed with 10% KOH(potassium hydroxide) + DMSO (di-methyl sulfonic acid). Presence of filaments confirmed under trinocular and sent for culture at PDA (potato dextrose agar). Fungal species identified under trinocular and confirmation was done through ITS1 and 4 regions (Internal transcribe spacer). Disc diffusion along with 90 well microdilution CLSI M61, ed.2 (Clinical and standard laboratory institute) was performed to analyse AFST (Antifungal susceptibility test). Finally SQLE and MEP1-5 was multiplied ,sequenced and biofilm was also analyzed of higher MIC (minimum inhibitory conc) of dermatophytes. 
 
Result: MIC of Trichophyton, Epidermophyton and Microsporum (5-25 &micro;g/ml) for terbinafine, for Itraconazole (0.09-1.5 &micro;g/ml) and for Fluconazole (0.03-0.5&micro;l/ml) found much more higher than previously reported data. Point mutations previously reported were observed in SQLE (squalene epoxidase). MEP-3 (metalloprotease)was prominently found in all the genera followed by MEP-5 and MEP-4 was in least amount in most of the genus isolated from this area. Biofilms deplete by increasing conc (0.2-1.0) of SS14 in microtiter wells; found more effective with SS14 ,R-70, when studied under trinocular and spectroscope.

Multilocus Phylogenetic Study Defining Resistance by Biofilms, Metalloprotease and SQLE of Dermatophytes Isolated from Various Altitudes of Uttarakhand