Background: Sperata seenghala Sykes, 1839 locally known as guchi aor is an economically important catfish inhabit in the Gomati River of Tripura, India and it is noticed that significantly reducing its population in its natural habitat for the last few decades. Therefore, proper identification of suitable stock is urgent need for artificial breeding purposes. 
 
Methods: In this study, the morphometric variation of five S. seenghala populations of Gomati river were assessed with multivariate analyses, including principal component analysis (PCA) and cluster analysis (CA) to distinguish different populations. 
 
Result: Of the total 26 transformed truss measurements, 5 exhibited significant (p&lt;0.05) differences among populations for females and no such significant difference was observed for males. PCA performed on truss data accounted for a total variation of 91.01% and 98.08% by the first two principal components (PC1 and PC2) for male and female populations respectively. The UPGMA dendrogram showed that Sonamura population has the most morphologically different populations in comparison to other populations. The data support the existence of morphological variation across different study location. However, if these findings are supported by further molecular markers, this would be a strong indication of different stocks of this species in the Gomati river of Tripura. The results obtained may contribute to effective conservation and management practices of the studied fish S. seenghala in its natural habitat. 
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Morphometric Variations among Isolated Populations of Catfish Sperata seenghala Sykes, 1839 in Gomati River of Tripura, India: A Truss Network based Analysis