Background: Microsatellites are short repeat motifs consisting 1-6 base pair (bp) and have a high degree of length polymorphism and are useful to study the genetic diversity. In Chicken, no micro satellite markers were demarcated to different chromosomes, viz., 29, 34, 35, 36, 37, 38 and 39 in the genome. In the present study, we carried out chromosome-wise identification of SSRs that will be of great use in diversity and gene mapping studies in chicken. 
 
Methods: The perl script of MISA tool was used to screen the polymorphic microsatellites with different thresholds level. 
 
Result: We identified a total of 397877 SSRs from the latest chicken genome assembly (GRCg7b), of which mononucleotides, dinucleotides, trinucleotides, tetranucleotides, pentanucleotides and hexanucleotides composed of 307453 (77.27%), 40991 (10.30%), 23059 (5.79%), 16723 (4.20%), 8127 (2.04%) and 1524 (0.38%), respectively.

Complete Chromosome Wise Identification of SSRs in the Two Published Chicken Genome Assemblies

In-silico development of protein models in fenugreek (Trigonella foenum-graecum) has opened up new vistas using the modern computational tools. The identification of protein in fenugreek having homology with the protein domain of humans and E-coli shows that the database available on Apiaceae family are very low. The estimated molecular weight of identified for fenugreek AM-1 protein was 11372.8 and was predicted as basic. A channel in fenugreek transmembrane protein has been identified through which a ligand (an ion or a small molecule) might pass. The present finding may be a valuable addition to the proteomic information available on fenugreek. Further validation can be performed using wet lab experiments.

Complete Chromosome Wise Identification of SSRs in the Two Published Chicken Genome Assemblies

Secondary and tertiary structure prediction of fenugreek (Trigonella foenum-graecum) protein