<div>Sarcocystis is one of the most prevalent parasites of the livestock and the infections in the intermediate hosts are mainly asymptomatic. The main aim of this study was to develop a sensitive antemortem serodiagnostic test Dot-ELISA for bovine sarcocystosis. The Dot-ELISA was optimized by using 1ng of particulate bradyzoite antigen (1.5% formalin fixed) per dot,1:32 dilution of serum and 1:100 dilution of Rabbit anti bovine IgG HRPO conjugate. The efficacy of this test was compared with Indirect Fluorescent Antibody Technique (IFAT) by using 45 known positive and 42 known negative sera. The sensitivity and specificity of Dot-ELISA was found to be 86.66% and 88.88% respectively and for IFAT the sensitivity was 83.33% and specificity was 100%. The standardized Dot-ELISA was used to screen 232 bovine sera (151 cattle, 81 buffaloes) collected from areas in and around Hyderabad, India which showed an overall prevalence of 82.75%. &nbsp;Around 143 out of 151 cattle and 49 out of 81 buffaloes were found positive indicating 94.7% and 60.49% of prevalence respectively. Dot-ELISA was found to be quite sensitive and inexpensive test to screen the animals at slaughter house or herd level.</div>


Serodiagnosis of Bovine Sarcocystosis by Dot-ELISA