Low calorie carrot halwa was prepared by replacing sugar in recipe with saccharine at four different levels and the product was also subjected to physical and sensory qualities during storage. Stability of saccharine was also studied during storage. It was found that carrot halwa prepared with replacement of sugar up to 10% with saccharine scored highest in sweetness perception and at par in other sensory properties i.e. colour and appearance, body and texture and overall acceptability with control. Storage studies at 10 &ordm;C revealed that saccharine sweetened carrot halwa (stored in polystyrene thermoformed cups), resembled the control in retaining the sensory profile, but showed increased acidity and microbial load. HPLC analysis showed no degradation of saccharine during entire period of storage in all samples.&nbsp;

Stability of saccharine in low calorie carrot halwa

Present research was conduct to build up an efficient protocol for various concentrations of 2, 4-D for &rdquo;Callus formation and somatic embryogenesis in sugarcane (saccharin spp L.) Using various concentrations of 2, 4-D and RAPD analysis of regenerated plants in the Laboratory of Biotechnology Nuclear Institute of Agriculture Tandojam during the year 2013- 2014. Three sugarcane varieties Bl4, NIA-2010, NIA-2011 with various concentrations of 2, 4-D (0.5.1.0, 2.0, 3.0 and 4.0 mg l-1). While 3.0 mg l-1 Kin+IAA+IBA were used for callus proliferation and shoot formations. Four different concentration of IBA were used for rooting purpose 0.5, 1.0, 2.0 and 3.0 mgl-1+20 g Sugar. Embryonic callus was obtained by culturing young apical merited. Eight-month field grown three sugarcane clones via; BL4, NIA-2010 and NIA-2 011 we use for tissue culture somatic embryonic callus studies. The apical meristematic region was used for callus formation and somatic embryogenesis induction on 0.5, 1.0, 2.0, 3.0 and 4.0 mg l-1.2, 4-D actively growing callus was subcultured on kin.IAA, IBA, 3.0 mg l-1. Maximum callus proliferation and a number of plantlets shoot length and regeneration growth was observed in those plants that call taken from 1.0 and 2.0 mg l-1.2, 4-D Maximum chlorophyll mutation frequency was recorded in NIA-2010 and BL4 grown on 1.0 mg l-1 2, 4-D. The maximum number of roots was observed in BL4 when 1.0 mg l-1IBA+ 20% sugar was applied, variability obtained through callus culture and also confirmed through random amplified polymorphic DNA (RAPD) techniques.

Somatic embryogenesis and callus formation in sugarcane (Saccharum SPP L.) using different concentration of 2, 4-D and RAPD analysis of plants regenerates