Background: Renal ischemia reperfusion injury (IRI) can be frequently observed in mandatory clinical situations such as organ transplantation, as well as in undesirable clinical scenarios such as embolic, thrombotic causes and hypotensive shock. This damage is an important pathological process that can occur in a wide clinical spectrum, such as acute renal failure and permanent renal failure. The effects of Quercetin and Resveratrol, substances with high antioxidant capacity that may have a positive effect on this pathological process, on renal IRI were examined by looking at Raftlin and 8-iso Prostaglandin F2alpha (8-iso-PGF2a) levels. 
 
Methods: Forty-two male Wistar-albino rats were divided into six groups (Control group; n=7, I/R group; n=7, Sham group; n=7, Quercetin group; n=7, Resveratrol group; n=7, Quercetin and resveratrol group; n=7). Quercetin (100 mg/kg) and resveratrol (100 mg/kg) was administered intragastricly in the treatment group. Only sham group was administered with 0.9% serum physiologic (NaCI). Raftlin and 8-iso-PGF2a values were measured by ELISA method. In the histopathological examination, the groups were evaluated in terms of hemorrhage, myocardial edema, myocytolysis and polymorphonuclear leukocyte infiltration. 
 
Result: It was observed that both quercetin and resveratrol molecules decreased Raftlin and 8-iso-PGF2a values. The group in which the two molecules were given together had the lowest values and it was determined that this value approached the control group value. Quercetin and resveratrol are thought to have a protective effect on renal IRI.

Effect of Quercetin and Resveratrol on Raftlin, 8-iso Prostaglandin F2alpha Levels in Renal Ischemia-reperfusion Injury

<div>Background: The incidence and complications of bladder disorders due to ischemia-reperfusion (I/R) injury constitute an important public health problem. The harmful effects of the disease could be reduced or completely eliminated by using natural antioxidant agents. In this study, the effects of preconditioning with Resveratrol&nbsp; and B17 vitamin, on bladder I/R damage was evaluated by the levels of 8-iso-prostaglandin F2&alpha; (8-iso-PGF2&alpha;) and raftlin-1 (RFTN-1) biomarkers.&nbsp; 
 
Methods: A total of 42 Wistar Albino male rats were used in the study which were divided in 6 groups (Group 1: Control, Group 2: Sham, Group 3: I/R, Group 4: Treatment 1 (RSV), Group 5: Treatment 2 (B17), Group 6: Combined treatment (RSV+B17). The effect of RSV and B17 valuated as the therapeutic group. Clamping the penile urethra of the rats, diuresis was forced and overdistension was achieved. After being kept in overdistension for 30 minutes, the rat bladder was emptied with a 3F catheter. The reperfused bladder was kept in this way for 30 minutes and the rats were sacrificed. 8-iso-PGF2&alpha; and RFTN-1 levels were measured in bladder tissue by ELISA. 
 
Result: In the I/R rat bladder tissue, we detected severe tissue injuries (p&lt;0.05). As compared to the control group; It was observed that 8-iso-PGF2&alpha; and RFTN-1 values increased statistically significantly in sham, I/R and treatment 2 groups (p&lt;0.05). 8-iso-PGF2&alpha; and RFTN-1 values were statistically significantly decreased in the therapeutic groups (Treatment 1, Treatment 2, Combined treatment compared to the I/R and sham group (p&lt;0.05). A statistically significant decrease was observed in the treatment 1 group compared to the treatment 2 group in terms of 8-iso-PGF2&alpha; and RFTN-1 values (p&lt;0.05).</div>

Protective Effect of Resveratrol and Vitamin B17 on 8-iso-Prostaglandin F2&alpha; and Raftlin-1 Levels in an Experimental Acute Urinary Retention in Rat