Background: The rising concerns over antibiotic resistance and the growing demand for antibiotic-free poultry products have driven interest in natural alternatives like postbiotics. These bioactive compounds support gut health, enhance immunity and improve meat quality without compromising production efficiency. By reducing antibiotic dependence, postbiotics contribute to one health initiatives, minimizing antibiotic residues in the food chain and mitigating environmental contamination. This approach promotes sustainable poultry production, enhances food safety and aligns with global efforts to combat antimicrobial resistance. In this context, the present study evaluates the impact of postbiotic supplementation on chicken meat quality parameters. 
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Methods: Postbiotic metabolites were extracted from Lactobacillus acidophilus using MRS broth culture and centrifugation, followed by the collection of cell-free supernatants. These postbiotics were analysed for their physiochemical properties and then mixed with feed daily during the feeding trial. A total of 480, Day old CARI-Bro Dhanraja chicks were randomly assigned to six separate groups i.e. T1-Basal diet (BD) supplemented with 0.2% (v/w) MRS Broth or uninoculated media; T2-BD+Antibiotic (CTC@335 mg/kg); T3-BD+ Probiotic; T4-BD+Post biotics @ 0.2% (v/w); T5-BD+Post biotics @ 0.4% (v/w) and T6-BD+Post biotics @ 0.6% (v/w). 
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Result: The study demonstrated that post biotic supplementation significantly (P&lt;0.001) improved meat quality indicators in broiler chickens, reducing cooking loss, drip loss and shear force, leading to more tender and juicier meat. Carotenoid deposition significantly improved (p&lt;0.001), enhancing meat appearance. Sensory evaluation showed no significant differences in appearance and colour, but T4 consistently received the highest scores for flavour, odor, juiciness and overall acceptability. The results suggest that postbiotics derived from Lactobacillus acidophilus at the dose of 0.2% can enhance meat tenderness, moisture retention and carotenoid deposition, potentially improving the overall quality of broiler meat can be used as a substitute for antibiotics in diets of broiler chickens. 
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Impact of Lactobacillus acidophilus-Derived Post Biotic Metabolites on Performance and Meat Quality in Broiler Chickens