Background: The porcine sapelovirus (PSV) is a small, non-enveloped, single-stranded, positive-sense, RNA virus of the family Picornaviridae. The PSV infections in pigs have been found associated with diarrhoea, polioencephalomyelitis, pneumonia and reproductive disorders with a high morbidity rate. Despite of its economical importance very few studies are available on the pathology of PSV. The present study was conducted with the aim to investigate the PSV infection and associated pathology in Indian pigs.&nbsp; 
 
Methods: Tissue samples along with intestinal content were collected from a total of 78 necropsied cases for histopathological examination and molecular investigation during April 2019 to August 2020. The amplification of 5&#39; UTR region of PSV was carried out via RT-PCR and confirmed by sequencing. The Genetic characterization of Indian isolate of the PSV was done on the basis of viral 5&#39; UTR gene.&nbsp; 
 
Result: A total of eight out of 78 cases were found positive for the PSV. Catarrhal and haemorrhagic enteritis, thickening and clouding of brain meninges along with congestion of brain and pneumonia was observed as common gross lesions. Microscopic lesions included perivascular cuffing, focal gliosis, neuronophagia, congestion of meningeal and cerebral vessels, interstitial pneumonia, inflammatory changes in the intestinal mucosa and sloughing of villi. The genetic characterization revealed maximum identity of 96.89% with PSV-1 strain PSV-46-V (LC508233) and PSV-1 strain PSV-26-B (LC508232) of Zambia. This study reported the pathological and molecular investigation of PSV from Indian pigs. Further explorative surveillance along with experimental studies in suitable animal model and cell lines are highly warranted for better understanding of PSV pathology in Indian pigs.

Pathology and Molecular Characterization of Porcine Sapelovirus in Indian Pigs

Background: A total of 20 tissue samples from horn, eye, skin and vulva in bovines suspected of squamous cell carcinoma (SCC) on the basis of gross pathology were collected from Durg, Dhamtari and Rajnandgaon districts of Chhattisgarh and processed in the Department of Veterinary Pathology, College of Veterinary Science and A.H., DSVCKV, Anjora, Durg (C.G.). 
&nbsp; 
Methods: Investigation was conducted during January to June 2022, we explicate the pathological and immunohistochemical examination including scoring and grading of squamous cell carcinoma in bovines. Histopathological examination as per standard HandE method of staining and immunohistochemistry was performed through Benchmark automated staining system. 
&nbsp; 
Result: Twelve cases were diagnosed as SCC on the basis of histopathological and eleven on the basis of immunohistochemical analysis. Grossly neoplasms of horn revealed large, cauliflower like unilateral irregular masses at the base of horn and they were friable and soft in consistency. Small, unilateral growths observed in eye varied from soft to hard in consistency. Histopathological findings in SCC of horn revealed keratinization of horn epithelium forming concentric keratin pearls. Distinctive keratin pearls with more keratin deposition towards the center, numerous mitotic figures with anaplasia and neovascularization were evident in OSCC. Immunohistochemistry revealed strong immunohistochemical expression of Pan-Cytokeratin (Pan-CK), p53 and EGFR and negative&nbsp; to p16. Immunohistochemical scoring (IHS) was done in Pan-CK, EGFR and p53, ranged from 1-4. Well differentiated SCC of horn revealed strong immunoexpression against EGFR, varied from mild to intense. SCC of horn exhibited strong immunoexpression for Pan-CK, p53 and EGFR whereas, immunoexpression in OSCC specifically p53 was less as compared to SCC of horn. Grading and differentiation of SCC (horn and eye) was also done on basis of histopathological and immunohistochemical scoring.

Pathology and Diagnosis of Squamous Cell Carcinoma in Bovines

<div>Background: Trichomonas gallinae is a flagellated protozoa, infects the upper digestive system of pigeon and causing a condition called canker, characterized by development of granulomatous lesions in the buccal cavity, crop, proventriculus, gizzard and liver of affected birds causing mortality upto 90% in squabs. Systemic research on prevalence and pathology of T. gallinae in pigeons are scant in India. The present work was carried out to document the prevalence and pathology of T. gallinae in pigeons of Assam. 
 
Methods: During the one- year study period, throat swab samples were collected from households, market places and temple premises in 4 districts of Assam. A total of 324 birds were examined by wet mount and Giemsa staining methods for detection of T. &nbsp;gallinae. Naturally died carcasses were studied for gross and histopathological alteration in different organs. 
 
Result:&nbsp;In the present study prevalence of T. gallinae was found 26.85% and squabs were highly susceptible with 56.25 percent. &nbsp;Sex wise, comparatively higher prevalence was observed in females (33.54%) than in the males (20.48%). Seasonality study revealed highest prevalence during winter. Gross pathological alteration characterized by yellowish white caseo-necrotic haemorrhagic lesion in the upper digestive tract beginning with the oral cavity and lesions also found in liver and lungs. Histopathological alteration also recorded in different organs. In the liver sinusoidal congestion and kupffer cell hyperplasia were observed. Infiltration of polymorphonuclear cells in the thickened inter alveolar space of lungs also recorded. Further study is required to identify the prevalent strains of T. gallinae on the basis of pathogenicity in this geographic region and immunology of T. gallinae in pigeons and other hosts remain to be explored.</div>

<div>Prevalence and Pathology of Trichomonas gallinae in Domestic Pigeon (Columba livia domestica) of Assam, India</div>

Background: The porcine teschovirus (PTV) is common swine pathogen which causes a wide range of illnesses ranging from asymptomatic infection to acute fatal encephalomyelitis, diarrhoea and pneumonia. Despite of its economical importance very limited studies are available on the pathology of PTV. The present study was conducted with the objective to investigate the PTV infection and associated pathology in piglets. 
 
Methods: A total of 78 piglets below 3 months of age were necropsied and representative tissue samples along with intestinal content were collected for histopathological examination and molecular investigation during April 2019 to November 2020. The 5&#39;&nbsp; UTR region of PTV was amplified via RT-PCR and further confirmed by sequencing. Thereafter, genetic characterization of the Indian PTV isolate was done on the basis of 5&#39; UTR gene.&nbsp; 
 
Result: A total of seven out of 78 cases were found positive for the PTV. On necropsy the intestinal wall was thinned and distended with yellowish coloured diarrhoeic content. Congestion of serosal and mucosal vessels along with severe meningeal congestion was observed. Microscopic lesions included congestion of mucosal vessels and destruction of villous structure of jejunum along with perivascular cuffing, focal gliosis, neuronophagia, congestion of meningeal and cerebral vessels and interstitial pneumonia. The phylogenetic analysis revealed that the isolate PTV/India/IVRI-381/2020 (MW022462) and PTV/India/IVRI-1093/2020 (MW022463) were clustered with PTV-13 strain wild boar/WB2C-TV/2011/HUN (JQ429405) and PTV-2 strain Vir 6711-12/83 (AF296107) of Hungary and Germany respectively. This study reported the genotypic and pathological investigation of PTV from Indian piglets. Further explorative surveillance along with studies in suitable cell lines and animal model will pave the way for better understanding of PTV among Indian pigs.

Genetic Characterization and Pathology of Porcine Teschovirus (PTV) in India

<div>Background: A slaughter house study helps in the assessment of the disease status of herds and contain valuable informations about the incidence and epidemiology of animal diseases. Abattoir study also plays a significant role to know about the extent of exposure of the public to certain zoonotic diseases and helps in estimation of the financial losses due to condemnation of affected organs.&nbsp;Even in India, there is a lack of data from a proper slaughterhouse study of cows in Mizoram to determine the prevalence of pathological lesions affecting the organs and corpses. 
 
Methods: Total of 90 samples of each organ i.e. liver, lungs, kidneys, heart, spleen and 35 intestine samples were collected during the period from March 2019 to March 2020 for the present study from the Govt. slaughterhouse, Aizawl, Mizoram. Representative tissue samples were collected in 10% neutral buffered formalin (NBF) for further histopathological analysis. Fixed tissue samples processed through paraffin embedding technique and sections were cut at a 4-5 m thickness, stained with H and E and special staining like Masson&rsquo;s trichrome, Luna-Ishak, Von Kossa and Perl&rsquo;s.&nbsp; 
 
Result: Among all the organs studied, liver (65.55%) showed the highest prevalence of pathological lesions followed by intestine (42.86%), lungs (38.88), kidneys (35.55%), heart (31.11%) and spleen (20%). Circulatory disturbances were the most common pathological lesion encountered in all the organs. The major pathological condition encountered in liver was hepatitis and fibrosis. Interstitial pneumonia was more prevalent in the lungs than bronchopneumonia. Glomerulonephritis was more common in the kidney than tubulo-interstitial nephritis. In the heart, Sarcocyst was more prevalent than myocarditis.</div>

Pathological Studies on Specimens Collected from Slaughtered Bovines in Aizawl District of Mizoram

<div>Background: Odisha experiencing sporadic outbreaks of Peste des petits ruminants (PPR) throughout the year. There is a scarcity of available literature on PPR in Odisha till today. This is the first ever detail investigative approach in the state undertaken with an objective to corelate the epidemiological risk factors, haemato-biochemical and pathological changes in natural field outbreaks occurring in eight different districts.&nbsp;&nbsp; 
 
Methods: Fourteen field outbreaks of PPR were evaluated clinically as well as epidemiologically and confirmed through polymerase chain reaction (PCR). Blood, serum, faecal and tissue samples were collected to observe haemato-biochemical and pathomorphological changes to asses disease severity. 
 
Result: Present study concluded an overall mortality rate of 46.81%. Chi-square analysis revealed significant highest prevalence among 7-12 months (46.13%) age, Ganjam breed (45.51%) and females (80.49%). Frequent migration among the border areas along with poor management and helminthic infection was major precipitating factor. There was polycythemia along with neutrophilia and lymphopenia. Significant increase in alanine transaminase (ALT), aspartate aminotransferase (AST), K+ and Ca2+&nbsp;along with creatinine, urea and blood urea nitrogen (BUN) BUN was observed in affected flocks. Antero-ventral consolidation of lungs, syncytia and presence of both eosinophilic intranuclear and intracytoplasmic inclusion bodies were major pathological changes.&nbsp;</div>

Epidemiology, Haemato-biochemical and Pathological Changes Related to Field Outbreaks of PPR in Small Ruminants in Odisha

<div>Background: Infectious laryngotracheitis (ILT) is an economically important viral respiratory disease in poultry. Recently, re-emergence of Infectious laryngotracheitis virus (ILTV) has been reported in several countries including India. The current study aimed to evaluate the poultry flocks of Tamil Nadu with circulating GaHV-1 and to elucidate the origin of the virus involved in the outbreak. 
 
Methods: In this study, a molecular based survey on the overall occurrence of natural cases of Infectious laryngo-tracheitis in poultry flocks from Tamil Nadu, India were performed. Pathological findings in respiratory and secondary lymphoid organs like caecal tonsils and harderian gland was carried out. The PCR technique targeting Infected Cell Protein-4 (ICP4) gene along with molecular characterization was performed. 
 
Result: The overall prevalence rate in the outbreak was 42.86% with highest incidence in layer flocks (62.85%) than the broiler flocks (22.85%). The highest susceptible age groups were between 20-30 weeks old. Tracheal pathology revealed epithelial sloughing, syncytial cell formation, eosinophilic intranuclear inclusion bodies and heterophilic exudation microscopically. Partial genome sequencing and phylogenetic analysis of ICP4 gene revealed high genetic homology between field isolates and the virulent strains from Turkey, Germany, China and Brazil. In the present study, along with pathological findings, a rapid and sensitive PCR assay was used for detection of ILT virus specific ICP4 gene in commercial poultry farms in the region.</div>

Pathological Diagnosis and Genomic Characterization of ICP4 Gene of lnfectious Laryngotracheitis Virus (ILTV) Isolates in Clinically Infected Chicken in Tamil Nadu, India

<div>Background: This study elucidated the molecular detection and pathological alterations in broiler chickens naturally infected with field circulating NDV strains along with their phylogenomic dynamics. 
 
Methods: Morbid tissue samples of diseased/dead chickens were collected from 100 poultry flocks presented to poultry disease diagnostic laboratories from September 2018 to August 2019. Samples were subjected to molecular detection of NDV along with phylogenetic analysis and subsequent gross and histopathological examination. 
 
Result: Based on RT-PCR results, the positivity of NDV was 04/100 (4%). Genetic analysis of the NDV Fusion (F) gene revealed 98.92% and 98.74% similarity with Iranian and Pakistani isolates, respectively. The evolutionary tree showed that present study isolates were placed in a clade belongs to genotype Vll sub-genotype i and l. Necropsy examinations revealed the petechial haemorrhages associated with multifocal necrosis in gastrointestinal and respiratory organs. Besides these pathological findings, amino acid sequence of F gene revealed that study isolates are having pathogenic potential similar to the velogenic strains of NDV. Based on all essential analyses, the present study concluded that the evolution and distribution of the Newcastle disease virus of various genotypes VIIi and VIIl in Pakistan are having significant pathogenic potential. Therefore, it emphasizes developing ND vaccine from indigenous strains for better protection of commercial poultry in Pakistan.</div>

Molecular Characterization and Pathology of Virulent Newcastle Disease Virus Isolated from Broiler Chickens in District Lahore, Pakistan

<div>Background: Postweaning multisystemic wasting syndrome (PMWS) is caused by porcine circovirus type 2 (PCV2). It is a globally emerging clinical condition which affects nursing piglets and growing pigs. PCV2 requires various cofactors for fully development of PMWS. The present report describes the pathology of PMWS associated with classical swine fever virus (CSFV), porcine parvo virus (PPV) and multidrug resistant enteric Escherichia coli infections in an organized swine farm, Rajasthan. In India, this is the first report on pathology of PMWS associated with all the three viruses and multidrug resistant enteric colibacillosis. 
 
Methods: Detailed necropsy was conducted on pig carcass submitted to Post-mortem Facility, Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar in the year 2017 from an organized swine farm, Rajasthan, India. Heart blood and diarrhoeic intestinal contents were collected aseptically and stored separately at 4&deg;C for bacteriological examination and antibiotic sensitivity test. Tissue samples were collected in sterile container and stored at -40&deg;C for DNA and RNA extraction and polymerase chain reaction (PCR) and also collected in 10% neutral buffered saline for histopathological processing. 
 
Result: The organized farm was having history of 33% (200/600) of mortality among 2 months to 6 months old piglets. Gross lesions were wasting, lymphadenopathy, non-collapsed lungs, hydropericardium, hepatomegaly, icterus, enteritis and pale kidneys, button shaped ulcers in the caecum and colon etc. Histopathologically, lymphoid depletion, reticuloendothelial cell proliferation in lymphoid tissue, fibrin thrombi in spleen, severe hepatitis, interstitial pneumonia, interstitial nephritis, severe necrosis in large intestine, meningitis, perivascular cuffing (PVC), gliosis, neuronal degeneration in cerebrum etc were observed. Molecular diagnosis and bacteriological examination confirmed presence of PCV2, PPV, CSFV and multidrug resistant Escherichia coli.</div>

Pathology of PMWS Associated with Classical Swine Fever Virus, Porcine Parvo Virus and Multidrug Resistant Enteric Escherichia coli Infections in an Organized Swine Farm, Rajasthan, India

<div>Background: Pyometra is a pathological state of the compromised uterus of grown-up to old, diestrum bitches. Pyometra is easy to identify but can be more challenging when there is no vaginal discharge. In the present study, an attempt was made to find out the pathogen involvement and clinico-haemato-biochemical alteration in bitches affected with Pyometra. \ 
 
Methods: Twelve bitches with a history of inappetence, polydipsia, dirty vaginal discharge, occasional vomition irrespective of breeds and age groups were clinically examined and diagnosed as pyometra. The presumptive clinical diagnosis was based on case history, clinical signs, vaginal cytology, abdominal ultrasonography or radiography, gross examination of a pus-filled uterus after the ovariohysterectomy. 
 
Result: Vaginal examination revealed brown chocolate malodorous vaginal discharge with discolouration of the perivulval tissue. Abdominal ultrasound showed hyperplasia with echogenic material in the uterine wall along with distended uterine horns containing a large volume of cellular contents. Radiographic imaging revealed cranio-dorsal displacement of the small intestine and a twisted uniform tubular opacity with a distended uterus. The vaginal cytology revealed a large number of degenerated neutrophils, intermediate and parabasal cells. Escherichia coli were the major pathogen isolated from the pyometra uteri of bitches. The surface of the endometrium was columnar ciliated epithelium, with hyperplasia of subepithelial tissue and thick muscularis. The haemato-biochemical evaluation in pyometra infected bitches revealed decreased haemoglobin (Hb), packed cell volume (PCV), total erythrocytic count (TEC) and lymphocyte count indicating the microcytic hypochromic type of anaemia. Leucocytosis with a predominant absolute neutrophilia and lymphocytosis were consistently found in canine pyometra. There was a significantly elevated profile of blood urea nitrogen, creatinine, transaminases and alkaline phosphatase as well as total proteins observed in pyometric female dogs as compared to healthy ones which may have diagnostic and prognostic importance, which also indicated damage of vital organ caused by bacterial endotoxins. The haemato-biochemical indices correlating with imaging techniques, vaginal cytology and histopathological examination can serve as good prognostic markers in bitches with pyometra.</div>