<p style="text-align:justify">The objective of the present work was to standardize and evaluate indirect plate and dot- enzyme linked immunosorbent assay (ELISA) using purified Paramphistomum epiclitum homologous antigens in the small ruminants. Electrophoretic separation of somatic antigen (PeSAg) in reducing condition on 15% polyacrylamide gel resolved into 16 proteins of the molecular weight ranging from 14 -100 kDa. Two step ethanolic precipitation of supernatant of in-vitro culture of the fluke yielded P. epiclitum excretory-secretory antigen (PeESAg) of molecular weight 28 kDa. The animals (Goats=123; Sheep=91) were broadly kept into post-mortem and faecal examined groups. At many occasion the PeSAg found to cross reacts with other helminths parasites thus minimizing the specificity of the tests and antigens. There was no any direct correlation between the parasites load and ELISA reactivity pattern. The noted prevalence rate after combining the results of post-mortem examination and PeESAg based ELISA (plate and paper/ dot) was 30.08% (37/123) in goats and 28.57% (26/91) in sheep. While using PeESAg, the calculated overall sensitivity% was 92.86 (goats)/ 100 (sheep) in both plate and dot-ELISA, specificity% was 91.58 (goats)/ 91.55 (sheep) in plate ELISA while 88.42 (goats)/ 92.96 (sheep) in dot-ELISA, positive predictive value% was 76.47 (goats)/ 76.92 (sheep) in plate ELISA while 70.27 (goats)/ 80 (sheep) in dot-ELISA and negative predictive value% was 97.75 (goats)/ 100 (sheep) in plate ELISA while 97.67 (goats)/ 100 (sheep) in dot-ELISA, these values were optimum for the field sera sample so the tests and PeESAg can be recommended for the detection P. epiclitum infection in the small ruminants.&nbsp;</p>


<p style="text-align: justify;">Immunodiagnostic potency of homologous antigens for natural Paramphistomum epiclitum infection in small ruminants in plate and paper enzyme linked immunosorbent assay</p>


<div>A total of 1169 dung samples from domestic ruminants were examined to record the prevalence of paramphistomosis infection. In cattle, the prevalence of infection by direct smear (DS) and sedimentation method (SD) was 17.43% and 31.19%, respectively. In sheep, the prevalence rates were recorded higher than cattle (26.09% and 33.18%). In goats, the prevalence of paramphistomosis was 20.66% and 30.52%. Out of 109 cattle carcasses, 47 were found with flukes in rumen, reticulum and bile duct during slaughterhouse examination (SH). In sheep and goat, the prevalence rates were 42.15% and 40.85%, respectively by SH. Age-wise the highest prevalence was recorded in cattle of 2-4 years (50.0%) by SH. In goats, the prevalence of infection was lower in the age group of &lt;1 year, when compared to their counterparts in sheep. In contrast, the higher prevalence was noticed in &gt;1-2 years old goats (63.27%) than sheep of the same age group (36.85%). Sex-wise, statistically no significant difference was observed between male and female animals. Morphologically identified, Cotylophoron cotylophorum, Paramphistomum cervi, Gastrothylax crumenifer, Fischoederius elongatus and Gigantocotyle spp. In conclusion, an overall prevalence of 24.29%, 32.51% and 42.0% of paramphistomosis infection was recorded in cattle, sheep and goats by DS, SD and SH examinations, respectively.&nbsp;</div>


Immunodiagnostic potency of homologous antigens for natural Paramphistomum epiclitum infection in small ruminants in plate and paper enzyme linked immunosorbent assay

Prevalence of Paramphistomosis in Domestic Ruminants in Chittoor District of Andhra Pradesh, India