<div>Background: Ticks and tick-borne diseases (TBDs) are considered to be the most serious health problems for animals and humans. This issue prompts veterinarians and researchers to adopt better preventive and control strategies. Therefore, this study aims to develop hybridoma vaccine against Hylomma dromedarii infesting Camelus dromedaries.&nbsp; 
 
Methods: Myeloma cell culture was administered to the immunized mouse in order to produce hybridomas cells. These cells were then emulsified with Montanide ISA-51 adjuvant. Two doses of the prepared anti-tick vaccine were administered intradermally to camels with an interval of five weeks. The humoral response of experimental animals was measured by indirect enzyme-linked immunosorbent assay (ELISA).&nbsp; 
 
Result:&nbsp;There was a significant (p&lt;0.05) decrease in immune responses in tick counts and molting capacity. Overall, 85% efficacy of the developed vaccine was recorded. It is concluded that hybridoma based vaccine is effective in controlling H. dromedarii infestation against camels.</div>

An Evaluation of Hybridoma based Anti-tick Vaccine against H. dromedarii Tick Infesting Camels

<div style="text-align: justify;">Most of the common viral diseases can be diagnosed by serological assays and these assays mostly depend on the purity and quality of antibody used. &nbsp;Such specific antibodies can be generated by hybridoma technology. Alternatively, the virus-specific antibodies can be purified from polyclonal serum by immunoaffinity purification technique. Based on this immune affinity purification technique we have purified group-specific antibody against Bluetongue virus (BTV) using recombinant protein VP7 of BTV bound to polystyrene wells. Elution buffer consisting of 100 mM Glycine-HCl, pH 3.0 was found optimum for dissociation of the antibody from recombinant antigen and also to maintain the integrity of antigen. The reactivity of eluted antibody was tested in enzyme-linked immunosorbent assay (ELISA). The purified antibody will be useful in other serological assays like ELISA, fluorescent assay, and agar gel immunodiffusion (AGID) for Bluetongue disease diagnosis.</div>

An Evaluation of Hybridoma based Anti-tick Vaccine against H. dromedarii Tick Infesting Camels

Immunoaffinity purification of bluetongue virus group specific antibody using recombinant protein adsorbed to polystyrene wells