<div><strong>Background: </strong>Halari donkey is a distinct breed of India that comes from the community of migrants from the regions of Halar, Bharwad and Rabaris of Gujarat. They have a brief history of travel and migration from arid and semi-arid regions of India. The population of Halari donkeys has fallen drastically in past and is further declining; therefore, these donkeys may be recognized as a threatened breed in near future.<br />
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<strong>Methods: </strong>The genetic diversity of this breed was determined using the D-loop gene of 542 bp size belonging to mitochondrial DNA and compared with the complete mitochondrial genome of <em>Equus asinus</em> (NC_001788.1). In the present study, a total of 8 haplotypes were identified with haplotype diversity of 0.8152 and nucleotide diversity of 0.1281 indicating that the population has high genetic diversity. The Median-joining network tree and neighbour-joining trees revealed close clustering of the Halari donkeys.<br />
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<strong>Result: </strong>The high nucleotide and haplotype diversity suggest Halari donkeys as a unique population and proper management and conservation is the need of the hour.</div>


<p>Mitochondrial DNA Variation and Genetic Relationships in Indian Halari Donkey Breed using D-Loop Region</p>


<div style="text-align: justify;">Over the last decade, research has been conducted towards the uses and properties of donkey milk that has distinct chemical composition and consequently particularly nutritional properties. It is interesting to determine antioxidant potential of indigenous Halari and French Poitu donkey milk by using the total antioxidant capacity and ferric reducing antioxidant power assay. These methods are reliable, fast and with sound methodological infrastructures. The objective of the present research was to investigate the applicability of the trolox based total antioxidant capacity (TAC) and ferric reducing ability of plasma (FRAP) assay for identification of antioxidant potential of Indian Halari donkey milk and French Poitu donkey milk. The donkey milk is quite popular for its anti-aging properties in Western and European countries as cosmetic and nutraceutical preparations. However, in Indian context no such study is conducted on Halari donkey milk. The Indian Halari donkeys are untapped resource for their dairy potential. Our results showed that the whole milk of Halari donkey is at par with French Poitu donkey milk in terms of their antioxidative potential.</div>

<div style="text-align: justify;">Estimation of Antioxidant Potential of Indigenous Halari and French Poitu Donkey Milk by using the Total Antioxidant Capacity and Ferric Reducing Antioxidant Power Essay</div>