<div>Background: Frizzled (FZD) family works with the ligand Wnt protein to mediate downstream signals in the Wnt signaling pathway, while playing a superior role in the body. Therefore, this study was carried out to detect the expression patterns of FZD4 at the gene and protein levels. 
 
Methods: 10 developing and postnatal geese dorsal skin of Anser anser (n=5) and Anser cygnoides (n=5). Quantitative real-time polymerase chain reaction (qPCR) and western blotting were used to detect relative expression levels of FZD4. Feather follicles membrane localization was examined using immunohistochemistry. Student&rsquo;s t-test and one-way ANOVA were used for statistical analysis. 
 
Result: The results revealed that the FZD4 was expressed differently at mRNA and protein levels at six stages. Furthermore, the positive expression sites of FZD4 protein were gradually transferred from dermal papilla; pulp; follicle wall and feather sheath. These results suggest that FZD4 is expressed in the growth and development of feather follicles for Anser anser and Anser cygnoides.&nbsp;</div>

​​Expression of Frizzled-4 Gene on Developing and Postnatal Anser anser and Anser cygnoides Feather Follicles

<div>Background: Since both fetal and postnatal development is determined by early embryonic development and the molecular mechanism and organogenesis process of early mouse embryos have been thoroughly studied. However, the specific development process of early embryos in Mongolian sheep remains poorly understood.&nbsp; 
 
Methods: The 16-day and 25-day embryo samples of Inner Mongolia Hulunbuir short-tailed sheep were evaluated by transcriptome sequencing technique and the genes with significant differences were screened and the gene function was enriched by gene ontology (GO) database and Kyoto Encyclopedia of Gene and Genome (KEGG) database. 
 
Result: A total of 4348 differentially expressed genes (DEGs) were analyzed by sequencing and especially significant DEGs included the genes such as COL6A2, COL4A4, LAMA4, ITGA8, FN1 involved in ECM-receptor interaction, focal adhesion, PI3K-Akt signaling and protein digestion and absorption pathways; YAP1, FZD4, TGFB1, WNT2, BMP5, BMP2, WNT11 involved in Hippo signaling pathway. In pathway analysis, those pathways mainly related to cell interaction and organogenesis were highlighted at the E16 stage. They are critical for transcriptional control in early embryonic development, especially during organ building. The genes that make organs and body structures more complex and detailed and those related to the development of brain, liver and immune system are enriched for the E25 stage. Therefore, this study provides important supplementary and exploratory reference data for the further research on early embryonic development in sheep.</div>

​​Expression of Frizzled-4 Gene on Developing and Postnatal Anser anser and Anser cygnoides Feather Follicles

The Transcriptional Landscape of the Early Embryonic Developmental Biology in Hulunbuir Short-Tailed Sheep

Expression of Frizzled-4 Gene on Developing and Postnatal Anser anser and Anser cygnoides Feather Follicles