Background: The aim of current investigation was to analyze synergistic effect of natural penetration enhancers in combination with a synthetic drug in an animal model to enhance transdermal drug delivery. 
 
Methods: Initially, while carrying out the work in 2021-23 trial formulation batches were prepared with different penetration enhancers and then these were undergone through optimization study to optimize various parameters. A 32 full factorial design was utilized for study of optimization. An appropriate animal model was selected to analyze anti-inflammatory and analgesic effect of product. Statistical analysis was doneby utilizing one-way ANOVA, post hoc Tukey-Kramer test utilizing GraphPad Prism 9. Value of p&lt;0.05 wasdeemed considerable. 
 
Result: It was concluded from the study that reduction in paw licking activity was approximately found to be 69.52, 72.32 and 74.10 % with Emulgel 1, Emulgel 2 and Emulgel 3, compared with group of control (p&lt;0.05 vs control). These findings showed that the analgesic effect of all three formulations was promising. Emulgel 3 i.e., Sapindus mukorossi showed better anti-inflammatory activity in comparison to Allium sativum and Zingiber officinale.

Investigating the Synergistic Potential of Combination of Natural Penetration Enhancers and a Synthetic Drug in Animal Model for Improved Transdermal Delivery

Background: Drug aceclofenac is commonly used as an analgesics both topically and orally. Though it has diverse side effects related to oral delivery. Aim of recent work is to design and optimize a transdermal gel loaded with penetration enhancer from various natural sources so as to promote the delivery via skin. 
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Methods: Carbopol was used as a gelling agent. Other ingredients used include essential oil from Allium sativum, Zingiber officinale, Triethanolamine, tween 80 etc. For animal studies Albino Wistar rats were employed in which analgesic as well as anti-inflammatory activity of Aceclofenac were evaluated. 
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Result: All the prepared Emulgel were good in appearance and viscous in nature. The pH of the Emulgel from both essential oils ranged between 5.00-7.00. The spreading coefficient ranged between 19.00 - 28.00 gm.cm/sec for FA1-FA9 and between 17-26.4 gm.cm/sec for FZ1-FZ9. All the formulations showed optimum viscosity. The drug release percent of Emulgel FA1-FA9 ranged between 75.0-89.0% and 80-90 for FZ1-FZ9. In in-vivo studies comparable analgesic and anti-inflammatory activity were found. As a concluding statement we can say that essential oil from both Allium sativum and Zingiber officinale can act as a potential candidate for enhancement of drug delivery through skin.

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Investigating the Synergistic Potential of Combination of Natural Penetration Enhancers and a Synthetic Drug in Animal Model for Improved Transdermal Delivery

Phytochemical and Pharmacological Screening of Natural Oils to Study Their Synergistic Activity with Aceclofenac