Background: Eri silkworm rearing, crucial to Assam&rsquo;s economy, faces severe productivity threats from disease and crop loss. These challenges impact farmers&rsquo; livelihoods, highlighting the urgent need for innovative solutions to boost disease resistance and crop yield, ensuring the industry&rsquo;s sustainability and profitability.&nbsp; 
 
Methods: The study investigated the detection of Lactobacillus species in the midgut of the Eri silkworm (Samia ricini, Donovan) collected from Borduar, Assam. The research involved identifying Ligilactobacillus salivarius in the midgut of 5th instar larvae of the Borduar ecorace of Eri silkworm. The larvae, reared separately on castor and kesseru host plants, were utilized for isolating Lactobacillus on MRS agar. Molecular identification of these isolates was achieved through 16S rRNA sequence analysis. 
 
Result:&nbsp;Ligilactobacillus salivarius, a species under the genus Lactobacillus, was identified for the first time in the midgut of Eri silkworm larvae. This bacterium is widely known as a potential probiotic in higher animals, particularly poultry, indicating its possible benefits for silkworm health. The discovery of Ligilactobacillus salivarius in silkworms paves the way for further research aimed at developing novel probiotics tailored specifically for the sericulture industry, potentially improving silkworm health and boosting productivity. Implementing these probiotics presents a promising solution to the challenges facing sericulture in Assam, offering enhanced disease resistance and crop yield.

Detection of Probiotic Bacteria Ligilactobacillus salivarius in the Midgut of Eri Silkworm (Samia ricini, donovan) Collected from Borduar, Assam