<div>Background: It is not clear whether there are other motifs on the BMPIR gene in plateau-type Tibetan sheep and whether there is a strong association between the mutant locus and reproductive performance.&nbsp; 
 
Methods: Single-nucleotide polymorphisms (SNPs) of the BMPRIB gene in the Tibetan sheep populations from the Qinghai pastoral area in China were detected by Sanger sequencing and performed correlation analysis with litter size.&nbsp; 
 
Result: Four SNPs (g.597 G&gt;A, g.746 A&gt;G, g.864 C&gt;T and g.1113 C&gt;A) of BMPRIB gene were firstly screened in Tibetan sheep. In loci g.746, relevance analysis discovered that genotypes GG and AG had 0.70 (P&lt;0.05) and 0.79 (P&lt;0.05) litter sizes more than the genotype AA in Tibetan sheep, respectively. In loci g.597, Tibetan sheep with genotypes AA and GA had 0.21 (P&lt;0.05) and 0.31 (P&lt;0.05) litter sizes more than the genotype GG, respectively. The results showed that g.746 A&gt;G and g. 597 G&gt;A were regarded as assisted-markers in molecular. These findings provide ideas and insights into the reproductive performance of Tibetan sheep in China.</div>

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Single-nucleotide Polymorphism Scanning of Bone Morphogenetic Protein Receptor Gene and its Correlation with the Prolificacy of Plateau Tibetan Sheep

Background: This study aims to unravel the genetic intricacies regulating the reproductive prolificacy of Tibetan sheep, an indigenous breed prominently found in the Qinghai-Tibet Plateau.&nbsp; 
 
Methods: We employed meticulous sequencing and analytical strategies to discern the differential expressions of long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in the ovarian tissues of ewes with varying lambing numbers.&nbsp; 
 
Result: In our study, we identified 817 genes with differential expression in twin-bearing ewes compared to those bearing single lambs. This group comprised 495 upregulated and 322 downregulated genes, featuring key candidates like COL1A1, COL1A2, BMPRIB, GDF9, BMP15 and TGFB2. Furthermore, we discovered a notable difference in the expression of long non-coding RNAs (lncRNAs) in ewes birthing twins as opposed to singles, with 210 differentially expressed lncRNAs identified, including 91 upregulated and 119 downregulated. Key genes and lncRNAs associated with reproductive efficiency were identified, contributing valuable insights into the underlying molecular mechanisms and offering a robust foundation for enhancing breeding strategies and overall livestock productivity.

Single-nucleotide Polymorphism Scanning of Bone Morphogenetic Protein Receptor Gene and its Correlation with the Prolificacy of Plateau Tibetan Sheep

Elucidating the Genetic Regulation of Reproductive Prolificacy in Tibetan Sheep Through Ovarian Tissue Sequencing