Background: The safety of laboratory mice plays an important role in the success of laboratory experiments for correct and accurate results. Parasites are one of the most common diseases that affect most organisms. The worm Aspiculuris tetraptera is a common intestinal parasite of Mus musculus and rats, it is spread around the world. Aspiculuris tetraptera infection remains a problem for modern research groups; They should be excluded and monitored in rat populations due to their effects on animal health. 
 
Methods: The present study was conducted to assess the anthelmintic activity of Laurus nobilis extract in mice naturally infected with Aspiculuris tetraptera. Used was Thirty-six adult male and female (C57BL/6) mice, naturally infected with Aspiculuris tetraptera, were divided into six groups, each comprising six mice: Group 1, was the negative control (infected, untreated) and Groups 2, 3, 4 and 5 were treated with 50, 100, 200 and 400 &micro;g/ml of L. nobilis for 5 days respectively. Group 6 was treated with 10 mg/mL Albendazole for 3 days as a positive control. Different six treatments were used to test Aspiculuris tetraptera worms in vitro, Containing 4 concentrations (50, 100, 200 and 400 mg/ml) of L. nobilis extract. Ten actively moving adult worms were then placed in each petri dish at room temperature. A saline solution and 10 mg/ml of Albendazole were prepared and used as negative and positive controls. After treatment, observations were made by recording the death time for worms at 20, 40, 80, 120 and 180 minutes. Worms are considered dead if they do not move for 30 seconds after touching their body parts using a surgical needle and the petri dish is shaken. 
 
Result: The analysis of phytochemicals by FT-IR for alcoholic extracts of L. nobilis extracts revealed the presence of 15% effective chemical ingredients responsible for killing worms&rsquo; activity. In vitro, worms died in 20, 40, 80, 120 and 180 minutes. Attained 96% and 100% after 180 minutes at the highest concentrations (200 and 400 mg/ml). At the same time, the untreated group lasted for long hours. The effects of the plant extract (Laurus nobilis) on Aspiculuris tetraptera worms were studied in vivo at therapeutic doses of 50, 100, 200 and 400 mg/mL. and the concentration of 400 ml/kg showed the most lethal effects for worms, infected mice that did not receive drugs were compared with the highest concentration of the extract and the reference treatment. The attention showed significant differences P&le;0.05. The results showed that the mortality rate of worms taken from the intestines of the treated mice that were slaughtered three and six sdays after giving the treatment reached 96% and 100% in the concentration of 400 ml/kg of the extract and 89% and 97%for the mice that treated in 10 ml/kg of met Albendazole respectively. This research showed that herbal remedies could lead to new parasitic disease drugs, and their derivatives can be used for medication production and bioactivity improvement.

The Assessment of the Anthelmintic Activity of Laurus nobilis Extract in Mice Naturally Infected with Aspiculuris tetraptera

Background: This study envisaged the development of functional beverages from the extracts of tropical spices using pineapple juice as a carrier and determination of the functional properties and the shelf life studies of the developed beverages. A functional beverage can be defined as &ldquo;any non-alcoholic drink that provides additional health benefits due to the inclusion of any bioactive component from a plant, animal, marine or microorganism source. Crude extracts (essential oils) were extracted using a microwave assisted extraction from dried Ginger, T. tetraptera and M. myristica and subsequently evaluated. Quantitative phytochemicals and antioxidant activities were determined in the crude extracts. 
 
Methods: Crude extracts were extracted using a microwave assisted extraction from dried Ginger, T. tetraptera and M. myristica and subsequently stored for further processing. Standard protocols were adopted for the processing of different extracts as well as in the beverage formulations. The physicochemical properties and the shelf life studies of the formulated beverage samples were determined using standard analytical procedures. 
 
Result: The physicochemical properties revealed that the TTA values ranged from 1.92 to 3.28 and were recorded by the control and AGM, respectively. The pH values ranged from 3.66 to 4.83 and were recorded by the control and sample AGM. The viscosity values also ranged from 69.45 to 90.41 and values were ascribed to the control and sample AGT. The control had the lowest TSS value while ATY gave the highest mean score of 4.60 for the TSS; the total sugars (brix) is highest for the control (7.17&deg;B) and least for sample ATM with a mean score of 2.94&deg;B. The shelf life studies of the formulated beverages revealed that the product containing no preservatives (citric acid), can stay on a shelf at room temperature for about four weeks and more than eight weeks in refrigeration condition.

Physicochemical and Shelf Life Studies of&nbsp; Functional Beverages Developed from Crude Extracts from Tropical Spices (Zingiber officinale, Monodora myristica and Tetrapluera tetraptera)

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The Assessment of the Anthelmintic Activity of Laurus nobilis Extract in Mice Naturally Infected with Aspiculuris tetraptera

Physicochemical and Shelf Life Studies of Functional Beverages Developed from Crude Extracts from Tropical Spices (Zingiber officinale, Monodora myristica and Tetrapluera tetraptera)