<div>Background: Chicken serum-mediated proliferation regulates chGRP78 to prevent apoptosis in chicken cells via chGRP78-mediated anti-apoptosis.&nbsp;However, the precise molecular mechanisms underlying the chGRP78-mediated protection against apoptosis remain undefined.&nbsp;In an earlier&nbsp;study, we have shown&nbsp;that chGRP78 is critical for chicken embryo fibroblast (CEF) and DF-1 cell proliferation. 
 
Methods: In this experiment, we highlight AKT1 as a key target of GRP78 during apoptosis. We used&nbsp;2D gel-based proteomics and bioinformatics prediction analysis&nbsp;for our studies.&nbsp; 
 
Result: Here,&nbsp;we detected chGRP78 binding sites in AKT1-rgulated proteins. chGRP78 promoted AKT1 activation and chGRP78 silencing decreased AKT1 levels. Taken together, we suggest that the AKT1-mediated signaling pathway plays a critical role in GRP78-stimulated fibroblast survival and anti-apoptosis.&nbsp;Our&nbsp;findings have important implications for the maintenance of chicken fibroblast cells via the inhibition of apoptosis.</div>

chGRP78 is Required for the Activation and Phosphorylation of AKT1 at Serine 478 in Chicken Sells