Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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  • Impact Factor 0.5 (2023)

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​Chip Detection of Rabbit Peripheral Blood Mononuclear Cells and 293T Cells Induced by Chicken Egg White Extract Shows Significant Differences in the Signaling Pathways that Regulate the Pluripotency of Stem Cells

Guang-ping Ruan, Xiang Yao, Ping Mo, Kai Wang, Jie He, Zai-ling Yang, Rong-qing Pang, Xiang-qing Zhu, Xing-hua Pan
Background: Stem cell protein chips can detect stem cell-associated proteins expressed in cells. 
Methods: We isolated and cultured rabbit peripheral blood mononuclear cells (R-PBMCs) in two bottles, one containing medium and one containing medium with 40% chicken egg white extract. Similarly, 293T cells were also divided into two vials: one vial contained medium and one vial contained medium with 40% chicken egg white extract. After 3 days of culture, proteins were extracted from the cells and used for the stem cell protein chip.
Result: Protein chip detection showed that 10 stem cell-associated proteins were increased in the rabbit peripheral blood mononuclear cells induced by chicken egg white extract. There were also 4 stem cell-associated proteins increased in the 293T cells induced by chicken egg white extract. The results of KEGG enrichment analysis indicated that peripheral blood mononuclear cells of rabbits were involved in the regulation of stem cell pluripotency before and after induction. Moreover, 293T cells were involved in the regulation of stem cell pluripotency before and after induction.

  1. Abeyrathne, E.D., Lee, H.Y. and Ahn, D.U. (2014). Separation of ovotransferrin and ovomucoid from chicken egg white. Poult Sci. 93: 1010-1017.

  2. Bhatt, J., Amarpal, Bhat, A.R., Singh, A.P., Pawde, A.M., Bhat, I.A., Sharma, G.T. (2021). Studies on the Efficacy of Single and Twice Application of Mesenchymal Stem Cells in Full Thickness Cutaneous Wound Healing. Indian Journal of Animal Research. 1330-1336.

  3. Bujun Mei, R.L. (2021). Identification of lncRNAs differentially expressed during natural and induced estrus in sheep. Indian Journal of Animal Research. 55: 1421-1429.

  4. Bupebayeva, L.K., Bartosz, M., Jan, M., Stanislaw, M. (2021). the effect of non-enzymatic glycosylation on the Physicochemical properties and immunoreactivity on Chicken egg ovomucoid. Indian Journal of Animal Research. 55: 1286-1292.

  5. Chang, L., Liu, Z., Zhao, Y., Li, Y., Qin, J. (2021). Establishment and preliminary application of multiplex fluorescent quantitative PCR for simultaneous detection of BVDV, BRV and BCV. Indian Journal of Animal Research. 55: 1141-1149.

  6. Fang, J., Ma, M.H., Qiu, N., Wu, X. and Jin, Y.G. (2012). Analysis of the low-molecular weight protein profile of egg-white and its changes during early chicken embryological development. Z Naturforsch C. 67: 208-214.

  7. Hansis, Barreto, C.G., Maltry, N. and Niehrs, C. (2004). Nuclear reprogramming of human somatic cells by xenopus egg extract requires BRG1. Curr Biol. 14: 1475-1480.

  8. Kodama, D., Nishimiya, D., Nishijima, K., Okino, Y., Inayoshi, Y., Kojima, Y., Ono, K., Motono, M., Miyake, K., Kawabe, Y., Kyogoku, K., Yamashita, T., Kamihira, M. and Iijima, S. (2012). Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system. J. Biosci Bioeng. 113: 146-153.

  9. Monika, M., Rokadae, J.J., Narayan, R., Verma, M.R., Panda, S., Saharia, J. (2021). Genetic evaluationof egg production and egg quality attributes in Japanese quails through partial periods. Journal of Animal Research. 55: 1393-1400.

  10. Miyamoto, K., Furusawa, T., Ohnuki, M., Goel, S., Tokunaga, T., Minami, N,. Yamada, M., Ohsumi, K. and Imai, H. (2007). Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts. Mol Reprod Dev. 74: 1268-1277.

  11. Miyamoto, K., Tsukiyama, T., Yang, Y., Li, N., Minami, N., Yamada, M. and Imai, H. (2009). Cell-free extracts from mammalian oocytes partially induce nuclear reprogramming in somatic cells. Biol Reprod. 80: 935-943.

  12. Mizutani, A., Tsunashima, H., Nishijima, K., Sasamoto, T., Yamada, Y., Kojima, Y., Motono, M., Kojima, J., Inayoshi, Y., Miyake, K., Park, E.Y. and Iijima, S. (2012). Genetic modification of a chicken expression system for the galactosylation of therapeutic proteins produced in egg white. Transgenic Res. 21: 63-75.

  13. Qiu, N., Liu, W., Ma, M., Zhao, L. and Li, Y. (2013). Differences between fertilized and unfertilized chicken egg white proteins revealed by 2-dimensional gel electrophoresis-based proteomic analysis. Poult Sci. 92: 782-786.

  14. Ruan, G.P., Wang, J.X., Yao, X., Pang, R.Q., Cai, X.M., He, J., Zhao, J. and Pan, X.H. (2014). Chicken egg extracts promote increased expression of pluripotent gene OCT4 and NANOG in 293T cells. Chinese Journal of Cell Biology. 36: 190-194.

  15. Ruan, G.P., Yao, X., Shu, J., Liu, J.F., Pang, R.Q. and Pan, X.H. (2017). Chicken egg-white extracts promote OCT4 and NANOG expression and telomeres growth in 293T cells. Cell Mol Biol (Noisy-le-grand). 63: 59-65.

  16. Takahashi, K.,Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K. and Yamanaka, S. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131: 861-872.

  17. Teshigawara, R., Cho, J., Kameda, M. and Tada, T. (2017). Mechanism of human somatic reprogramming to iPS cell. Lab Invest. 97: 1152-1157.

  18. Wang, L., Huang, D., Huang, C., Yin, Y., Vali, K., Zhang, M. and Tang, Y. (2017). Enhanced human somatic cell reprogramming efficiency by fusion of the MYC transactivation domain and OCT4. Stem Cell Res. 25: 88-97.

  19. Yang, Y., Barendregt, A., Kamerling, J.P. and Heck, A.J. (2013). Analyzing protein micro-heterogeneity in chicken ovalbumin by high-resolution native mass spectrometry exposes qualitatively and semi-quantitatively 59 proteoforms. Anal Chem. 85: 12037-12045.

  20. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L, Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, II and Thomson, J.A. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science. 318: 1917-1920.

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