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Current IssueEditorial BoardOnline First ArticlesArchive

Full Research Article

Successful Artificial Insemination using Sexed and Non Sexed Semen on Limousin Crossbred Cow

A
A.P.A. Yekti
J
J. Riyanto
C
C. Hanim
R
R. Prafitri
G
G. Ciptadi
S
S. Rahayu
T
T. Susilawati

ABSTRACT

Background: Beef cattle fattening business requires male cattle because they grow faster so that by using sexed semen (Y sperm) it is expected to get a male calf. The purpose of this study was to determine the success of artificial insemination using non-sexed and sexed frozen semen using percoll density gradient centrifugation (PDGC) method. 
Methods: A total of 120 female Limousin Crossbred were used in this study. The cows were raised on smallholder farms aged between 3-9 years with a body condition score (BCS) between 3-7 (score 1-9). The treatment was T0: 60 cows were inseminated using non-sexed frozen semen while T1: 60 cows were inseminated using sexed Y frozen semen. The insemination was performed with double doses at the 2nd and 8th hours after oestrus and deep insemination. The parameter measured were NRR-1, NRR-2, conception rate and pregnancy rates. Determination of pregnancy was performed using rectal palpation and ultrasonography (USG). 
Result: The results showed that NRR-1 and NRR-2 at T0 were 47 (78.33%) and 42 (70%), while at T1 were 37 (61.67%) and 35 (58.33%), respectively. While conception rate at T0 and T1 was 22 (36%) and 18 (30%), pregnancy rate at T0 and T1 was 31 (56%) and 19 (31.67%) and silent heat at T0 and T1 was 24 (40%) and 26 (43.3%).

REFERENCES


  1. Bajaj, N.K. and Sharma, N. (2011). Endocine Caises of Early Embryonic Death: An Overview. Current Research in Dairy Sciences. 3: 1-24. ISSN 1994-5434/DOI:10.3923/ crds.2011.1.24. 

  2. Boukari, F.Z.A., Alkoiret, I.T., Toleba, S.S., Ahissou, A., Toure, F.Z., Yacoubou, A.M., Bonou, G.A., Dotche, I.O., Akpaki, V., Karim, I.Y.A. (2018). Reproductive Performance of the Borgou Cow Inseminated on Natural or Induced Estrus with Semen from Gir and Girolando at the Okpara Breeding Farm. Veterinary World. 11: 693-699.

  3. Hafez, E.S.E. and Hafez, B. (2000). Reproductive Cycles. In: Reproduction in Farm Animals. Edited by B. Hafez and E.S.E. Hafez 7th ed. Lippincott. Williams and Wilkins. USA: 55-67.

  4. Hayakawa, H. (2012). Sperm Sexing In The Cattle Industry. Journal of Mammalian Ova Research. 29: 119-123. 

  5. Inchaisri, C., Jorritsma, R., Vernooij, J.C.M., Vos, P.L.A.M., Weijden, G.C.V.D., Hogeveen, H. (2011). Cow effects and estimation of success first and following inseminations in dutch dairy cows. Reproduction in Domestic Animals. 46: 1043-1049.

  6. Jaenudeen and Hafez. (2000). Pregnancy Diagnosis. In: Reproduction in Farm Animals. Edited by B. Hafez and E.S.E. Hafez 7th ed. Lippincott. Williams and Wilkins. USA: 395-404.

  7. Kusumawati, E.D., Isnaini, N., Rahayu, S., Yekti, A.P.A., Luthfi, M., Affandhy, L., Pamungkas, D., Kuswati, K., Ridhowi, A., Sudarwati, H., Susilawati, T. (2017). The Quality of Sexed Semen on Filial Ongole Bull Using Percoll Density Gradient. Asian Jr. of Microbiol. Biotech. Env. Sc. 19: 189-199.

  8. Promthep, K., Satitmanwiwat, S., Kitiyanan, N., Tantiwattanakul, P., Jirajaoenrat, K., Sitthigripong, R., Singhapol, C. (2016). Practical use of percoll density gradient centrifugation on sperm sex determination in commercial dairy farm in thailand. Indian Journal of Animal Research. 50: 310-313.

  9. Scolari, S.C., Clark, S.G., Knox, R.V., Tamassia, M.A. (2011). Vulvar Skin Temperature Changes Significantly During Estrus in Swine as Determined by Digital Infrared Thermography. Journal of Swine Health and Production. 19: 151-155.

  10. Susilawati, T., Kusumawati, E.D., Isnaini, N., Yekti, A.P.A., Sudarwati, H., Ridhowi, A. (2017). Effect of sexing process using percoll density gradient centrifugation and frozen on motility and demage to spermatozoa membrane of filial ongole. Advances in Health Sciences Research. 5: 227-231. 

  11. Susilawati, T., Rahayu, S., Udrayana, S., Sudarwati, H. (2014). Effect of different centrifugation on simmental bull sperm quality and membrane status after sexing, cooling and freezing process. American-Eurasian Journal of Sustainable Agriculture. 8: 28-34.

  12. Van De Gucht, T., Saeys, W., Van Nuffel, A., Pluym, L., Piccart, K., Lauwers, L., Vangeyte, J. and Van Weyenberg, S. (2017). Farmers’ preferences for automatic lameness-detection systems in dairy cattle. Journal of Dairy Science. 100: 5746-5757.

  13. Verma, K.K., Prasad, S., Kumaresan, A., Mohanty, T.K., Layek, S.S., Patbandha, T.K., Chand, S. (2014). Characterization of physico-chemical properties of cervical mucus in relation to parity and conception rate in murrah buffaloes. Veterinary World. 7: 467-471.

  14. William, S. and Swecker, Jr. (2014). Interaction of Nutrition and Reproduction in the Beef Cow Bovine Reproduction Ed by R.M. Hopper, India: Wiley Blackwell: 276-282. ISBN: 978-1-118-47083.
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Indian Journal of Animal Research
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    Full Research Article

    Successful Artificial Insemination using Sexed and Non Sexed Semen on Limousin Crossbred Cow

    A
    A.P.A. Yekti
    J
    J. Riyanto
    C
    C. Hanim
    R
    R. Prafitri
    G
    G. Ciptadi
    S
    S. Rahayu
    T
    T. Susilawati

    ABSTRACT

    Background: Beef cattle fattening business requires male cattle because they grow faster so that by using sexed semen (Y sperm) it is expected to get a male calf. The purpose of this study was to determine the success of artificial insemination using non-sexed and sexed frozen semen using percoll density gradient centrifugation (PDGC) method. 
    Methods: A total of 120 female Limousin Crossbred were used in this study. The cows were raised on smallholder farms aged between 3-9 years with a body condition score (BCS) between 3-7 (score 1-9). The treatment was T0: 60 cows were inseminated using non-sexed frozen semen while T1: 60 cows were inseminated using sexed Y frozen semen. The insemination was performed with double doses at the 2nd and 8th hours after oestrus and deep insemination. The parameter measured were NRR-1, NRR-2, conception rate and pregnancy rates. Determination of pregnancy was performed using rectal palpation and ultrasonography (USG). 
    Result: The results showed that NRR-1 and NRR-2 at T0 were 47 (78.33%) and 42 (70%), while at T1 were 37 (61.67%) and 35 (58.33%), respectively. While conception rate at T0 and T1 was 22 (36%) and 18 (30%), pregnancy rate at T0 and T1 was 31 (56%) and 19 (31.67%) and silent heat at T0 and T1 was 24 (40%) and 26 (43.3%).

    REFERENCES


    1. Bajaj, N.K. and Sharma, N. (2011). Endocine Caises of Early Embryonic Death: An Overview. Current Research in Dairy Sciences. 3: 1-24. ISSN 1994-5434/DOI:10.3923/ crds.2011.1.24. 

    2. Boukari, F.Z.A., Alkoiret, I.T., Toleba, S.S., Ahissou, A., Toure, F.Z., Yacoubou, A.M., Bonou, G.A., Dotche, I.O., Akpaki, V., Karim, I.Y.A. (2018). Reproductive Performance of the Borgou Cow Inseminated on Natural or Induced Estrus with Semen from Gir and Girolando at the Okpara Breeding Farm. Veterinary World. 11: 693-699.

    3. Hafez, E.S.E. and Hafez, B. (2000). Reproductive Cycles. In: Reproduction in Farm Animals. Edited by B. Hafez and E.S.E. Hafez 7th ed. Lippincott. Williams and Wilkins. USA: 55-67.

    4. Hayakawa, H. (2012). Sperm Sexing In The Cattle Industry. Journal of Mammalian Ova Research. 29: 119-123. 

    5. Inchaisri, C., Jorritsma, R., Vernooij, J.C.M., Vos, P.L.A.M., Weijden, G.C.V.D., Hogeveen, H. (2011). Cow effects and estimation of success first and following inseminations in dutch dairy cows. Reproduction in Domestic Animals. 46: 1043-1049.

    6. Jaenudeen and Hafez. (2000). Pregnancy Diagnosis. In: Reproduction in Farm Animals. Edited by B. Hafez and E.S.E. Hafez 7th ed. Lippincott. Williams and Wilkins. USA: 395-404.

    7. Kusumawati, E.D., Isnaini, N., Rahayu, S., Yekti, A.P.A., Luthfi, M., Affandhy, L., Pamungkas, D., Kuswati, K., Ridhowi, A., Sudarwati, H., Susilawati, T. (2017). The Quality of Sexed Semen on Filial Ongole Bull Using Percoll Density Gradient. Asian Jr. of Microbiol. Biotech. Env. Sc. 19: 189-199.

    8. Promthep, K., Satitmanwiwat, S., Kitiyanan, N., Tantiwattanakul, P., Jirajaoenrat, K., Sitthigripong, R., Singhapol, C. (2016). Practical use of percoll density gradient centrifugation on sperm sex determination in commercial dairy farm in thailand. Indian Journal of Animal Research. 50: 310-313.

    9. Scolari, S.C., Clark, S.G., Knox, R.V., Tamassia, M.A. (2011). Vulvar Skin Temperature Changes Significantly During Estrus in Swine as Determined by Digital Infrared Thermography. Journal of Swine Health and Production. 19: 151-155.

    10. Susilawati, T., Kusumawati, E.D., Isnaini, N., Yekti, A.P.A., Sudarwati, H., Ridhowi, A. (2017). Effect of sexing process using percoll density gradient centrifugation and frozen on motility and demage to spermatozoa membrane of filial ongole. Advances in Health Sciences Research. 5: 227-231. 

    11. Susilawati, T., Rahayu, S., Udrayana, S., Sudarwati, H. (2014). Effect of different centrifugation on simmental bull sperm quality and membrane status after sexing, cooling and freezing process. American-Eurasian Journal of Sustainable Agriculture. 8: 28-34.

    12. Van De Gucht, T., Saeys, W., Van Nuffel, A., Pluym, L., Piccart, K., Lauwers, L., Vangeyte, J. and Van Weyenberg, S. (2017). Farmers’ preferences for automatic lameness-detection systems in dairy cattle. Journal of Dairy Science. 100: 5746-5757.

    13. Verma, K.K., Prasad, S., Kumaresan, A., Mohanty, T.K., Layek, S.S., Patbandha, T.K., Chand, S. (2014). Characterization of physico-chemical properties of cervical mucus in relation to parity and conception rate in murrah buffaloes. Veterinary World. 7: 467-471.

    14. William, S. and Swecker, Jr. (2014). Interaction of Nutrition and Reproduction in the Beef Cow Bovine Reproduction Ed by R.M. Hopper, India: Wiley Blackwell: 276-282. ISBN: 978-1-118-47083.
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