Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Long-term Feeding Effects of Calcium-bentonite Clay in Aflatoxin B1 Contaminated Diet on Egg Production, Egg Quality and Immune Status of Laying Hens

E. Khamphong, C. Bunchasak, K. Poungpong, S. Prasongsook, T. Poeikhampha
Background: This experiment was conducted to determine the effect of synthetic aflatoxin B1 and calcium bentonite clay as a mycotoxin binder in the laying hen diet on performance, egg quality and immune response to vaccination.
Methods: A total of 144 Lohmann brown classic hens (22 weeks old) were divided into 4 groups of 6 replications with 6 hens each. This experimental design was completely randomized design and there were 4 experimental diets: (1) Basal diet (Positive control), (2) Basal diet +0.25% calcium bentonite clay, (3) Basal diet+200 ppb aflatoxin B1 (negative control) and (4) Basal diet+200 ppb aflatoxin B1 and +0.25% calcium bentonite clay. All hens were vaccinated with Newcastle (ND) and Infectious bronchitis (IB) vaccines during 24 weeks feeding trial.
Result: At the end of the 24 weeks of feeding trial, dietary treatments did not influence the egg production, egg mass, feed conversion ratio (FCR) and IB titer of hens; however, aflatoxin B1 significantly (P<0.05) deceased final body weight and deceased the eggshell color score (P<0.05). Moreover, the ND titer was reduced by aflatoxin B1 at 8th and 12th weeks of trial (P=0.05 and <0.05; respectively). Thus, supplementation of 0.25% calcium bentonite clay in feed improved body weight, eggshell color and ND titer of laying hens similar to those fed the positive control diet.

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